Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105570
Name   oriT_STLEFF_28|unnamed3 in_silico
Organism   Klebsiella quasipneumoniae strain STLEFF_28
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP058862 (47354..47454 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_STLEFF_28|unnamed3
TATTAATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGTTAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3792 GenBank   WP_058818671
Name   mobF_HZT23_RS27325_STLEFF_28|unnamed3 insolico UniProt ID   A0A6G8F840
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120149.39 Da        Isoelectric Point: 6.5766

>WP_058818671.1 MULTISPECIES: MobF family relaxase [Enterobacteriaceae]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKIVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A0A6G8F840


Auxiliary protein

ID   1721 GenBank   WP_001749975
Name   WP_001749975_STLEFF_28|unnamed3 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   3786 GenBank   WP_000342688
Name   t4cp2_HZT23_RS27330_STLEFF_28|unnamed3 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18893..29218

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT23_RS27165 (HZT23_27035) 13894..14946 + 1053 WP_239613368 alpha/beta fold hydrolase -
HZT23_RS27170 (HZT23_27040) 14888..15229 - 342 Protein_12 AraC family transcriptional regulator -
HZT23_RS27175 (HZT23_27045) 15262..15867 - 606 WP_000509966 recombinase family protein -
HZT23_RS27180 (HZT23_27050) 15962..18859 + 2898 WP_001553819 Tn3-like element Tn5403 family transposase -
HZT23_RS27185 (HZT23_27055) 18893..19888 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
HZT23_RS27190 (HZT23_27060) 19930..21090 - 1161 WP_032334995 type IV secretion system protein VirB10 virB10
HZT23_RS27195 (HZT23_27065) 21090..21974 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
HZT23_RS27200 (HZT23_27070) 21985..22683 - 699 WP_000646594 type IV secretion system protein virB8
HZT23_RS27860 22673..22831 - 159 WP_012561180 hypothetical protein -
HZT23_RS27205 (HZT23_27075) 22902..23942 - 1041 WP_001749958 type IV secretion system protein virB6
HZT23_RS27210 (HZT23_27080) 23958..24185 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
HZT23_RS27215 (HZT23_27085) 24193..24906 - 714 WP_001749960 type IV secretion system protein virB5
HZT23_RS27220 (HZT23_27090) 24924..27524 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HZT23_RS27225 (HZT23_27095) 27524..27841 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
HZT23_RS27230 (HZT23_27100) 27891..28184 - 294 WP_001749962 hypothetical protein virB2
HZT23_RS27235 (HZT23_27105) 28194..28475 - 282 WP_032335136 transcriptional repressor KorA -
HZT23_RS27240 (HZT23_27110) 28484..29218 - 735 WP_196320807 lytic transglycosylase domain-containing protein virB1
HZT23_RS27245 (HZT23_27115) 29261..29632 + 372 WP_206695657 H-NS family nucleoid-associated regulatory protein -
HZT23_RS27250 (HZT23_27120) 29642..29992 + 351 WP_240709519 hypothetical protein -
HZT23_RS27255 (HZT23_27125) 29989..30303 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
HZT23_RS27260 (HZT23_27130) 30417..30650 + 234 WP_001191790 hypothetical protein -
HZT23_RS27265 (HZT23_27135) 30700..31347 + 648 WP_015344958 restriction endonuclease -
HZT23_RS27270 (HZT23_27140) 31352..31558 + 207 WP_001749967 hypothetical protein -
HZT23_RS27275 (HZT23_27145) 31569..31841 - 273 Protein_34 IS1 family transposase -
HZT23_RS27280 (HZT23_27150) 31940..33373 + 1434 WP_001288432 DNA cytosine methyltransferase -
HZT23_RS27285 (HZT23_27155) 33407..34075 - 669 Protein_36 type II site-specific deoxyribonuclease -

Host bacterium

ID   6008 GenBank   NZ_CP058862
Plasmid name   STLEFF_28|unnamed3 Incompatibility group   IncN
Plasmid size   60679 bp Coordinate of oriT [Strand]   47354..47454 [+]
Host baterium   Klebsiella quasipneumoniae strain STLEFF_28

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -