Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105559
Name   oriT_pSLU-1913 in_silico
Organism   Salmonella enterica subsp. enterica serovar Montevideo str. USDA-ARS-USMARC-1913
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP025279 (36058..36146 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSLU-1913
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3787 GenBank   WP_001474686
Name   nikB_AW65_RS25850_pSLU-1913 insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   1719 GenBank   WP_001283947
Name   WP_001283947_pSLU-1913 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   3782 GenBank   WP_001289269
Name   trbC_AW65_RS25855_pSLU-1913 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86899.11 Da        Isoelectric Point: 6.7712

>WP_001289269.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTAKTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41524..82332

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW65_RS25855 (AW65_26355) 39240..41531 - 2292 WP_001289269 F-type conjugative transfer protein TrbC -
AW65_RS25860 (AW65_26360) 41524..42594 - 1071 WP_001474688 IncI1-type conjugal transfer protein TrbB trbB
AW65_RS25865 (AW65_26365) 42613..43821 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
AW65_RS25870 (AW65_26370) 44113..44265 + 153 WP_001303307 Hok/Gef family protein -
AW65_RS25875 (AW65_26375) 44337..44588 - 252 WP_001291965 hypothetical protein -
AW65_RS26305 45512..45688 - 177 WP_001054900 hypothetical protein -
AW65_RS25890 (AW65_26390) 46080..46289 + 210 WP_000062603 HEAT repeat domain-containing protein -
AW65_RS25895 (AW65_26395) 46361..47023 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
AW65_RS25900 (AW65_26400) 47094..49262 - 2169 WP_000698368 DotA/TraY family protein traY
AW65_RS25905 (AW65_26405) 49359..49943 - 585 WP_001037985 IncI1-type conjugal transfer protein TraX -
AW65_RS25910 (AW65_26410) 49972..51174 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
AW65_RS25915 (AW65_26415) 51141..51755 - 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
AW65_RS25920 (AW65_26420) 51755..54799 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
AW65_RS25925 (AW65_26425) 54889..55689 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
AW65_RS25930 (AW65_26430) 55673..55861 - 189 WP_001277255 putative conjugal transfer protein TraS -
AW65_RS25935 (AW65_26435) 55925..56329 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
AW65_RS25940 (AW65_26440) 56380..56907 - 528 WP_001055569 conjugal transfer protein TraQ traQ
AW65_RS25945 (AW65_26445) 56907..57611 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
AW65_RS25950 (AW65_26450) 57611..58900 - 1290 WP_001271997 conjugal transfer protein TraO traO
AW65_RS25955 (AW65_26455) 58903..59886 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
AW65_RS25960 (AW65_26460) 59897..60589 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
AW65_RS25965 (AW65_26465) 60586..60933 - 348 WP_001055900 conjugal transfer protein traL
AW65_RS25970 (AW65_26470) 60951..64718 - 3768 WP_001474691 LPD7 domain-containing protein -
AW65_RS25975 (AW65_26475) 64808..65359 - 552 WP_000014584 phospholipase D family protein -
AW65_RS25980 (AW65_26480) 65374..65664 - 291 WP_001299214 hypothetical protein traK
AW65_RS25985 (AW65_26485) 65661..66809 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
AW65_RS25990 (AW65_26490) 66806..67624 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
AW65_RS25995 (AW65_26495) 67621..68079 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
AW65_RS26005 (AW65_26505) 68549..69253 - 705 WP_001067855 IS6-like element IS26 family transposase -
AW65_RS26685 (AW65_26510) 69340..69654 - 315 WP_061342203 helix-turn-helix transcriptional regulator -
AW65_RS26015 (AW65_26515) 69641..70300 - 660 WP_001038045 tetracycline resistance transcriptional repressor TetR(C) -
AW65_RS26025 (AW65_26525) 70393..71571 + 1179 WP_084543822 tetracycline efflux MFS transporter Tet(C) -
AW65_RS26030 (AW65_26530) 71549..72121 - 573 Protein_81 histidine phosphatase family protein -
AW65_RS26035 (AW65_26535) 72181..73383 - 1203 WP_000976353 conjugal transfer protein TraF -
AW65_RS26040 (AW65_26540) 73469..74293 - 825 WP_001238927 conjugal transfer protein TraE traE
AW65_RS26045 (AW65_26545) 74444..75598 - 1155 WP_001139958 site-specific integrase -
AW65_RS26060 (AW65_26560) 76289..76537 + 249 WP_001349157 hypothetical protein -
AW65_RS26065 (AW65_26565) 76534..77826 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
AW65_RS26070 (AW65_26570) 77826..78482 - 657 WP_001193553 prepilin peptidase -
AW65_RS26075 (AW65_26575) 78467..79027 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
AW65_RS26080 (AW65_26580) 79037..79651 - 615 WP_000959785 type 4 pilus major pilin -
AW65_RS26085 (AW65_26585) 79669..80766 - 1098 WP_001208805 type II secretion system F family protein -
AW65_RS26090 (AW65_26590) 80779..82332 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
AW65_RS26095 (AW65_26595) 82343..82795 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
AW65_RS26100 (AW65_26600) 82782..84077 - 1296 WP_000752774 type 4b pilus protein PilO2 -
AW65_RS26105 (AW65_26605) 84070..85752 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
AW65_RS26110 (AW65_26610) 85766..86203 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
AW65_RS26115 (AW65_26615) 86203..87270 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   5997 GenBank   NZ_CP025279
Plasmid name   pSLU-1913 Incompatibility group   IncI1
Plasmid size   92439 bp Coordinate of oriT [Strand]   36058..36146 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Montevideo str. USDA-ARS-USMARC-1913

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -