Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105538
• Name: oriT_pSymB
• Organism: Sinorhizobium meliloti 2011
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_020560 (725929..725957 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_SM2011_RS09990_pSymB

• ID: 3777
• GenBank: WP_015456652
• Name: traA_SM2011_RS09990_pSymB
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 169835.58 Da
• Isoelectric Point: 9.5433

>WP_015456652.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIRGAGRSIVSAAAYRHRARMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLRAAIDGR
SVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDGN
PHIHLMTALRPLTEQGFGPKKVPVLGEDGEPLRVITPDRPNGKIVYKLWAGDKETIKAWKIAWAETANRH
LALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIAMYFAPADLARRQEMADRLLAEPGLLLKQL
GNERSTFDERDIAKALHRYVDDPVDFANIRARLMASDELVLLKPQQIDAETGKAKQPAVFTTREMLRLEY
AMAQSAEVLSRRKGFGVSNARAAAAVRSIETADTEKPFRLDLEQVDAVRHVTRDNAIAAVVGLAGAGKST
LLAAARAAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGREQLQRGDVLVIDEAGMVSS
QQMARVLKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAISERIGFAELAGVRRQRDAWARDASRLFARGKV
EEGLDAYAQQGRIVETETRAEIVDRIVADWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLNE
ALRNVMIGEGALTGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTIVSTGDRRG
DTLLSVRLDSGRDVVISQDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADLY
AAKEDFEAKPEWGRKPRVDHAAGVTGELVKEGMAKFRPNDEDADESPYADIRTDDGTVQRLWGVSLPKAL
KDAGVAEGDTITLRKDGVERVKVQVPIVDAQTGEKRFEERQVDRNVWSASQLETAAARQERIERESHRPQ
LFKQLVERLSRSGAKTTTLDFEDEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVAK
LWERASVALGFAIERERRVAYNEERTETLSTGIPSDGKYLVPPTTTFSRSVAEDARLAQLSSQRWKEREA
IVHPVLAKIYRDPDGALAALNALASDAAIEPRKLAEDLGKAPDRLGRLRGSELVVDGRAARDERTAATVA
LSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALSKTAMARLVEIEAVREQGGDDAYRTAFTYA
VEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERREKLTRLFAVIRRFAEEQHL
AERQDRSKIVAGASVELGKGTFAVLPMLAAVTEFKTTVDEEARERALAAPHYAHHRAALVETATRVWRDP
ADAIGKIEDLIVKGFAAERIAAAVTNDPAAYGALRGSDRIMDKLLAVGRERKGALQAVPEAASRIRSLGA
SYASALDAETRSITEERRRMAVAIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPRIAREFAKVSRA
LDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLTILQQAVRVQSSEKIVSERRQRAINQSRGIRM

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1308-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1046-1124)

• AAA domain (AAA_30) (390-577)

• MobA/MobL family (MobA_MobL) (16-248)

Protein structure

No available structure.

T4CP

tcpA_SM2011_RS35135_pSymB

• ID: 3772
• GenBank: WP_015456746
• Name: tcpA_SM2011_RS35135_pSymB
• UniProt ID: _
• Length: 946 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 946 a.a.
• Molecular weight: 101663.39 Da
• Isoelectric Point: 4.5783

>WP_015456746.1 FtsK/SpoIIIE domain-containing protein [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEES
RIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPAI
TESVAVAEEGPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMPL
EVAAVPPTVTEPVKEAARIPAPIAAAPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEPV
TAEPQDIAAEPAAAEAAALPAHQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPPI
GAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEF
EPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLAL
CLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIP
HLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTGE
PLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKA
NFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRPE
YLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVE
RMEKDGLVGPANHVGKREIIYGNRDNAPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (543-761)

• Ftsk gamma domain (FtsK_gamma) (871-930)

• FtsK alpha domain (FtsK_alpha) (434-534)

Protein structure

No available structure.

Host bacterium

• ID: 5976
• GenBank: NC_020560
• Plasmid name: pSymB
• Incompatibility group: -
• Plasmid size: 1683348 bp
• Coordinate of oriT [Strand]: 725929..725957 [+]
• Host baterium: Sinorhizobium meliloti 2011

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7, AcrVIB