Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105533 |
| Name | oriT_SA20031783|unnamed1  |
| Organism | Salmonella enterica subsp. enterica serovar Saintpaul strain SA20031783 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022492 (97753..97834 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_SA20031783|unnamed1
GGGACAGGATATGTTTTGTTGAACCGCCTGCACGCAGTCGGCTTCAAAAAACGTCCTGGTCAGGGCGAAGCCCCGACACCCC
GGGACAGGATATGTTTTGTTGAACCGCCTGCACGCAGTCGGCTTCAAAAAACGTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3774 | GenBank | WP_093980962 |
| Name | Relaxase_CHC80_RS24690_SA20031783|unnamed1 |
UniProt ID | _ |
| Length | 663 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 663 a.a. Molecular weight: 75876.19 Da Isoelectric Point: 6.0931
>WP_093980962.1 TraI/MobA(P) family conjugative relaxase [Salmonella enterica]
MIPIVPPKRRDGKSSFLTLVSYLTLREDVRLAEPVSPDAPVMRMSRSKEAIFDRLVDYIDRSQDAVTQTP
VKEFADGRSQVLSGNVACETNCFSLDTASAEMNMVAAQNTRCVDPVYHFILSWPAQDNPVDTDIFDCARY
AIQALGMKDHQFVTAIHRDTDHVHCHVAVNRINPISYRAANLYNDIDTLHKVCRFLELKYDYTPDNGAWV
NENGAVVRSKNDVKSIPRKARQLEYHSDKESLYSYAVNECRERIGEILGSGEASWETLHAELIRAGLEMR
EKGEGLAIYSRHDESVTPIKASTLHPDLTRQCLEKWLGDFEPSPEVINNFDEDGVYLGSNYPQEFVYDAR
AHVRDQGARSERRLARAVAREDLDARYKAYKNAWVRPKMPDTDSGIQFKALSNRYAWKKEQVRYVFDDPL
IRKLIYRTLELERQKEFEMLRDKVKAEKTQFYSRPENRRLSYWQWVERQAAAQDQAAISRLRGRAYKLKQ
KQRTPGLSENAIVCAVADDIATVNIDGFESRVNRDGVIQYLQDGHVVIQDSGERIEIADPYREDGIHISD
AMLIAEEKSGEKLVFSGDGRFVNSACDVVQWFNEGGEKPLPLTDPQQRVMAGYDKPETHADRPVMTHRIV
DEETYLASRQVARTGDESETDELKPGKNTHRLR
MIPIVPPKRRDGKSSFLTLVSYLTLREDVRLAEPVSPDAPVMRMSRSKEAIFDRLVDYIDRSQDAVTQTP
VKEFADGRSQVLSGNVACETNCFSLDTASAEMNMVAAQNTRCVDPVYHFILSWPAQDNPVDTDIFDCARY
AIQALGMKDHQFVTAIHRDTDHVHCHVAVNRINPISYRAANLYNDIDTLHKVCRFLELKYDYTPDNGAWV
NENGAVVRSKNDVKSIPRKARQLEYHSDKESLYSYAVNECRERIGEILGSGEASWETLHAELIRAGLEMR
EKGEGLAIYSRHDESVTPIKASTLHPDLTRQCLEKWLGDFEPSPEVINNFDEDGVYLGSNYPQEFVYDAR
AHVRDQGARSERRLARAVAREDLDARYKAYKNAWVRPKMPDTDSGIQFKALSNRYAWKKEQVRYVFDDPL
IRKLIYRTLELERQKEFEMLRDKVKAEKTQFYSRPENRRLSYWQWVERQAAAQDQAAISRLRGRAYKLKQ
KQRTPGLSENAIVCAVADDIATVNIDGFESRVNRDGVIQYLQDGHVVIQDSGERIEIADPYREDGIHISD
AMLIAEEKSGEKLVFSGDGRFVNSACDVVQWFNEGGEKPLPLTDPQQRVMAGYDKPETHADRPVMTHRIV
DEETYLASRQVARTGDESETDELKPGKNTHRLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3767 | GenBank | WP_093980961 |
| Name | trbC_CHC80_RS24665_SA20031783|unnamed1 |
UniProt ID | _ |
| Length | 731 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 731 a.a. Molecular weight: 83011.52 Da Isoelectric Point: 7.4995
>WP_093980961.1 F-type conjugative transfer protein TrbC [Salmonella enterica]
MTTGNTQPVDKDRVRQITRSTWMDDYLLAPATVQAGLVVILVLTFLRTWILIPGVFATAIWLLTFFGQLF
RMPLRMPKDIGGIDMTTERENALEFKGPMGLFRFTRRRLTWDKSAGIMCLGHARRRFLGRELWLTRDDCL
RHMQLLATTGSGKTEALLSLQLNSLCMGRGMMFSDGKAETRLAYAIWSLMRRYGQEDNYYVLNFLNGGRD
RFDELLGNDRTRPQSNTINFFGDTTATFIIQLMESLLPQVGSNEAGWQDKAKPMLYGLVYALYYKCRKDN
IRLSQSIIQKHLPLKEMADLYIEAKQNGWHDEAVNALEAYLSNLAGFRMELVSKSSEWDQGVYDQHGFLT
QQFSRMLAMFNDVYGHIFSSDAGDIDISDVLHNDRTLVVLIPALELSKSESANLGKLYISAKRMVIARDL
GYQLEGKSRDVLTSKKFWSPFPFPDVHDELGSYFAPGMDDLAAQMRSLGIMLVISAQDIQRFVAQFKGEY
QTVNANTLVKWFMAMQDEKDTFELATATAGKDYFAELGEMKRTPGTVSSSYEEANTTYIREKNRITLDEL
KDLNPGEGFISFKSALVPSSAIYIPDDEKISSKLSLKINRFIDTGTPTEADLVAQNPHLKRLLPPSELEV
SMLLQRTDEIIVGSETHTLPPLMDPILARLMKVALDLDNRCDISYTPTQRGILLFEAARDVLKKRGKKWF
TVKTQPNPLRVSDAMAHRLARKSTAFMPQEQ
MTTGNTQPVDKDRVRQITRSTWMDDYLLAPATVQAGLVVILVLTFLRTWILIPGVFATAIWLLTFFGQLF
RMPLRMPKDIGGIDMTTERENALEFKGPMGLFRFTRRRLTWDKSAGIMCLGHARRRFLGRELWLTRDDCL
RHMQLLATTGSGKTEALLSLQLNSLCMGRGMMFSDGKAETRLAYAIWSLMRRYGQEDNYYVLNFLNGGRD
RFDELLGNDRTRPQSNTINFFGDTTATFIIQLMESLLPQVGSNEAGWQDKAKPMLYGLVYALYYKCRKDN
IRLSQSIIQKHLPLKEMADLYIEAKQNGWHDEAVNALEAYLSNLAGFRMELVSKSSEWDQGVYDQHGFLT
QQFSRMLAMFNDVYGHIFSSDAGDIDISDVLHNDRTLVVLIPALELSKSESANLGKLYISAKRMVIARDL
GYQLEGKSRDVLTSKKFWSPFPFPDVHDELGSYFAPGMDDLAAQMRSLGIMLVISAQDIQRFVAQFKGEY
QTVNANTLVKWFMAMQDEKDTFELATATAGKDYFAELGEMKRTPGTVSSSYEEANTTYIREKNRITLDEL
KDLNPGEGFISFKSALVPSSAIYIPDDEKISSKLSLKINRFIDTGTPTEADLVAQNPHLKRLLPPSELEV
SMLLQRTDEIIVGSETHTLPPLMDPILARLMKVALDLDNRCDISYTPTQRGILLFEAARDVLKKRGKKWF
TVKTQPNPLRVSDAMAHRLARKSTAFMPQEQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52371..89751
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CHC80_RS24440 (CHC80_24520) | 47468..48460 | + | 993 | WP_093980925 | toxin co-regulated pilus biosynthesis Q family protein | - |
| CHC80_RS24445 (CHC80_24525) | 48460..48891 | + | 432 | WP_093980926 | type IV pilus biogenesis protein PilM | - |
| CHC80_RS24450 (CHC80_24530) | 48906..50528 | + | 1623 | WP_093980927 | PilN family type IVB pilus formation outer membrane protein | - |
| CHC80_RS24455 (CHC80_24535) | 50533..51816 | + | 1284 | WP_093980928 | type 4b pilus protein PilO2 | - |
| CHC80_RS24460 (CHC80_24540) | 51959..52339 | + | 381 | WP_276208980 | type IV pilus biogenesis protein PilP | - |
| CHC80_RS24465 (CHC80_24545) | 52371..53870 | + | 1500 | WP_093980930 | ATPase, T2SS/T4P/T4SS family | virB11 |
| CHC80_RS24470 (CHC80_24550) | 53863..54978 | + | 1116 | WP_162983483 | type II secretion system F family protein | - |
| CHC80_RS24475 (CHC80_24555) | 55005..55598 | + | 594 | WP_093980932 | type 4 pilus major pilin | - |
| CHC80_RS24480 (CHC80_24560) | 55612..56082 | + | 471 | WP_047596209 | lytic transglycosylase domain-containing protein | virB1 |
| CHC80_RS24485 (CHC80_24565) | 56082..56693 | + | 612 | WP_093980933 | prepilin peptidase | - |
| CHC80_RS24490 (CHC80_24570) | 56792..58105 | + | 1314 | WP_093980934 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| CHC80_RS25345 (CHC80_24585) | 59321..59578 | - | 258 | Protein_61 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| CHC80_RS24505 (CHC80_24590) | 59663..60817 | + | 1155 | WP_093980935 | site-specific integrase | - |
| CHC80_RS24510 (CHC80_24595) | 61195..61650 | + | 456 | WP_093980936 | DotD/TraH family lipoprotein | - |
| CHC80_RS24515 (CHC80_24600) | 61647..62468 | + | 822 | WP_093980937 | type IV secretory system conjugative DNA transfer family protein | traI |
| CHC80_RS24520 (CHC80_24605) | 62465..63634 | + | 1170 | WP_093980938 | plasmid transfer ATPase TraJ | virB11 |
| CHC80_RS24525 (CHC80_24610) | 63624..63890 | + | 267 | WP_023208014 | IcmT/TraK family protein | traK |
| CHC80_RS24530 (CHC80_24615) | 63902..67339 | + | 3438 | WP_093980939 | DUF5710 domain-containing protein | - |
| CHC80_RS24535 (CHC80_24620) | 67355..67825 | + | 471 | WP_093980940 | hypothetical protein | traL |
| CHC80_RS24540 (CHC80_24625) | 67889..68566 | + | 678 | WP_093980941 | DotI/IcmL/TraM family protein | traM |
| CHC80_RS24545 (CHC80_24630) | 68563..69582 | + | 1020 | WP_093980942 | DotH/IcmK family type IV secretion protein | traN |
| CHC80_RS24550 (CHC80_24635) | 69582..70781 | + | 1200 | WP_093980943 | conjugal transfer protein TraO | traO |
| CHC80_RS24555 (CHC80_24640) | 70781..71530 | + | 750 | WP_093980944 | hypothetical protein | traP |
| CHC80_RS24560 (CHC80_24645) | 71540..72070 | + | 531 | WP_023208021 | conjugal transfer protein TraQ | traQ |
| CHC80_RS24565 (CHC80_24650) | 72086..72484 | + | 399 | WP_042313644 | DUF6750 family protein | traR |
| CHC80_RS24570 (CHC80_24655) | 72517..73092 | - | 576 | WP_137429010 | hypothetical protein | - |
| CHC80_RS24580 (CHC80_24665) | 73751..74017 | + | 267 | WP_093980946 | hypothetical protein | - |
| CHC80_RS24585 (CHC80_24670) | 74028..74612 | - | 585 | WP_093980947 | YkgJ family cysteine cluster protein | - |
| CHC80_RS24590 (CHC80_24675) | 74719..75327 | + | 609 | WP_093980948 | hypothetical protein | traT |
| CHC80_RS24595 (CHC80_24680) | 75324..78386 | + | 3063 | WP_093980949 | conjugal transfer protein | traU |
| CHC80_RS24600 (CHC80_24685) | 78492..79625 | + | 1134 | WP_240456750 | conjugal transfer protein TraW | traW |
| CHC80_RS24605 (CHC80_24690) | 79622..80140 | + | 519 | WP_093980951 | conjugal transfer protein TraX | - |
| CHC80_RS24610 (CHC80_24695) | 80192..82327 | + | 2136 | WP_093980952 | DotA/TraY family protein | traY |
| CHC80_RS24615 (CHC80_24700) | 82341..82892 | + | 552 | WP_093980953 | hypothetical protein | - |
| CHC80_RS24620 (CHC80_24705) | 83055..83765 | + | 711 | WP_307721961 | DotA/TraY family protein | - |
| CHC80_RS24625 (CHC80_24710) | 83774..84268 | + | 495 | WP_137429008 | hypothetical protein | - |
| CHC80_RS24630 (CHC80_24715) | 84310..84855 | + | 546 | WP_093980955 | hypothetical protein | - |
| CHC80_RS24635 (CHC80_24720) | 84940..85263 | - | 324 | WP_001125908 | CcdB family protein | - |
| CHC80_RS24640 (CHC80_24725) | 85263..85511 | - | 249 | WP_093980956 | type II toxin-antitoxin system CcdA family antitoxin | - |
| CHC80_RS25410 | 86189..86284 | + | 96 | WP_021542841 | DinQ-like type I toxin DqlB | - |
| CHC80_RS25415 | 87114..87209 | + | 96 | WP_000609145 | DinQ-like type I toxin DqlB | - |
| CHC80_RS24650 (CHC80_24735) | 87353..88669 | + | 1317 | WP_240456751 | conjugal transfer protein TrbA | trbA |
| CHC80_RS24655 (CHC80_24740) | 88723..89751 | + | 1029 | WP_093980959 | thioredoxin fold domain-containing protein | trbB |
| CHC80_RS24660 (CHC80_24745) | 89784..90914 | - | 1131 | WP_121526166 | RNA-guided endonuclease TnpB family protein | - |
| CHC80_RS24665 (CHC80_24750) | 91023..93218 | + | 2196 | WP_093980961 | F-type conjugative transfer protein TrbC | - |
| CHC80_RS24670 (CHC80_24755) | 93248..93793 | + | 546 | WP_079826998 | phospholipase D family protein | - |
| CHC80_RS24675 (CHC80_24760) | 93852..94334 | + | 483 | Protein_96 | transposase | - |
Host bacterium
| ID | 5971 | GenBank | NZ_CP022492 |
| Plasmid name | SA20031783|unnamed1 | Incompatibility group | - |
| Plasmid size | 131583 bp | Coordinate of oriT [Strand] | 97753..97834 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Saintpaul strain SA20031783 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | kexD |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |