Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105509 |
| Name | oriT_AR_0127|unnamed2  |
| Organism | Salmonella enterica subsp. enterica serovar Senftenberg strain AR_0127 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP032193 (66745..66850 [-], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_AR_0127|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3751 | GenBank | WP_015058937 |
| Name | Relaxase_AM374_RS01360_AR_0127|unnamed2 |
UniProt ID | A0A7G3L766 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75223.04 Da Isoelectric Point: 9.9948
>WP_015058937.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETVLAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNLPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETVLAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNLPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L766 |
T4CP
| ID | 3745 | GenBank | WP_011091092 |
| Name | t4cp2_AM374_RS01110_AR_0127|unnamed2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 44531..63949
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM374_RS01245 (AM374_01245) | 40353..41213 | + | 861 | WP_000027057 | broad-spectrum class A beta-lactamase TEM-1 | - |
| AM374_RS01250 (AM374_01250) | 42217..43272 | - | 1056 | WP_011091086 | plasmid replication initiator RepA | - |
| AM374_RS01255 (AM374_01255) | 43570..43800 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| AM374_RS01260 (AM374_01260) | 43875..44528 | - | 654 | WP_011091084 | hypothetical protein | - |
| AM374_RS01265 (AM374_01265) | 44531..46711 | - | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| AM374_RS01270 (AM374_01270) | 46704..47354 | - | 651 | WP_011091083 | hypothetical protein | - |
| AM374_RS01275 (AM374_01275) | 47351..48559 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| AM374_RS01280 (AM374_01280) | 48556..51606 | - | 3051 | WP_011091081 | hypothetical protein | traU |
| AM374_RS01285 (AM374_01285) | 51603..52097 | - | 495 | WP_015243645 | hypothetical protein | - |
| AM374_RS01290 (AM374_01290) | 52143..52532 | - | 390 | WP_011091078 | DUF6750 family protein | traR |
| AM374_RS01295 (AM374_01295) | 52549..53079 | - | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| AM374_RS01300 (AM374_01300) | 53103..53807 | - | 705 | WP_015243644 | conjugal transfer protein TraP | traP |
| AM374_RS01305 (AM374_01305) | 53819..55168 | - | 1350 | WP_015058942 | conjugal transfer protein TraO | traO |
| AM374_RS01310 (AM374_01310) | 55180..56331 | - | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| AM374_RS01315 (AM374_01315) | 56340..57053 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| AM374_RS01320 (AM374_01320) | 57121..57531 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AM374_RS01325 (AM374_01325) | 57560..57715 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AM374_RS01330 (AM374_01330) | 57716..58228 | - | 513 | WP_015058940 | hypothetical protein | traL |
| AM374_RS27305 | 58194..61721 | - | 3528 | WP_015058939 | LPD7 domain-containing protein | - |
| AM374_RS01340 (AM374_01340) | 61746..62006 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AM374_RS01345 (AM374_01345) | 61996..63159 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| AM374_RS01350 (AM374_01350) | 63170..63949 | - | 780 | WP_015058938 | type IV secretory system conjugative DNA transfer family protein | traI |
| AM374_RS01355 (AM374_01355) | 63946..64446 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| AM374_RS01360 (AM374_01360) | 64460..66439 | - | 1980 | WP_015058937 | TraI/MobA(P) family conjugative relaxase | - |
| AM374_RS27310 | 66426..66953 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| AM374_RS01370 (AM374_01370) | 66988..67383 | + | 396 | WP_015058936 | hypothetical protein | - |
| AM374_RS01375 (AM374_01375) | 67884..68420 | - | 537 | WP_015058935 | hypothetical protein | - |
| AM374_RS01380 (AM374_01380) | 68553..68864 | - | 312 | WP_015058934 | hypothetical protein | - |
Host bacterium
| ID | 5947 | GenBank | NZ_CP032193 |
| Plasmid name | AR_0127|unnamed2 | Incompatibility group | IncL/M |
| Plasmid size | 87450 bp | Coordinate of oriT [Strand] | 66745..66850 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Senftenberg strain AR_0127 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |