Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105503
• Name: oriT_pL21581-2
• Organism: Carnobacterium maltaromaticum strain TMW 2.1581
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP016846 (13818..13866 [+], 49 nt)
• oriT length: 49 nt
• IRs (inverted repeats): 7..14, 19..26 (GCAACATG..CATGTTGC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 49 nt

>oriT_pL21581-2
AAAATCGCAACATGGGGACATGTTGCTCTGCTCGCAAAAGGAAAGTCTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_BFC23_RS16525_pL21581-2

• ID: 3746
• GenBank: WP_119907100
• Name: Relaxase_BFC23_RS16525_pL21581-2
• UniProt ID: _
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 64630.93 Da
• Isoelectric Point: 6.2156

>WP_119907100.1 relaxase/mobilization nuclease domain-containing protein [Carnobacterium maltaromaticum]
MVYTKHFVIHTFDQLHDAEKYITDAMKTIVEKSDASSHLNTLFRYVASDDKTMMKQLVSSHGIIDIEDAY
EEFKYTKLQSAYKKGNSFSFDSKNGRMRKPTLSDLEKNNAVLAHHLIQSFSPDDPLTPEQIHEIGRKTVL
ELTGGTHEFVIATHVDKDHIHNHIVFNSTNMKSGNAFRWQKGTKNVYEQISDKIASKEGAKIIEKSPKNS
HKKYTMYQTESIFKQKIKSRLDFLMDHSSSLDDFLIKAEALELSVDFSKKWSTFRLLDEPQVKNTRGRSL
FKKDPNRYDLEKIVDHINMNTETFTVEDVVSRYEEKVNRKENDFDYQITLESWQISHKTTKGYYLNVDFG
FGNHGQLFIGAYKVDALDNGDYNLFVKRNDYFYFMNNKKADRSRYMTGETLIKQLQLYNGVTPLKKEPVM
STIDELVDAINFLAEHEITDGRQLNILEEKLENAFTEAEAALETLDEKIIELHQVGKLLLEAETNEDKAE
TQDKLKAFVPNAILSEITFEDVQSEISSASKSLELLETKLEQTVKKMNHLHEIQAVTEKNQEKPTQPRL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-283)

Protein structure

No available structure.

T4CP

t4cp2_BFC23_RS16500_pL21581-2

• ID: 3738
• GenBank: WP_119907095
• Name: t4cp2_BFC23_RS16500_pL21581-2
• UniProt ID: _
• Length: 605 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 605 a.a.
• Molecular weight: 69252.28 Da
• Isoelectric Point: 6.1130

>WP_119907095.1 VirD4-like conjugal transfer protein, CD1115 family [Carnobacterium maltaromaticum]
MQPYKKNFKPYLILAIILSMVGFVLGNFLWLLPGYDIYSKLMFLFNKDVSGFVNDSILQIFFTPSLFAFG
VGMLGFFGGLLAYVRNNDRGVYRLGEEYGSARFATTEEIVKFADKEKPENDLIYTRHARMCLFNSRLAFE
FQKNKNAVVIGGPGSGKTFTFIKPNIMQMNASFIVTDPKKLLVHETGKMLKDNGYKIKIFDLATLANSDS
FNVFNYIKTELDVDRVLEAITEGTKKNDQQGGEDFWRQAEALLIRSFIAYLWFDGQENDYTPHLGMIADM
LRLTERKDPKVPSPVEMWFEEQNERIPNNYAYKQFTLFNDLYKSETRMSVLAIAAGRYSIFDHEQVVDMV
REDTMDIESWNEEKTAVFIAIPETSDTYNFLSAIFLATAMEVLRKKADEVLTGERILAIGKKLLHIRFLI
DEFANIGRIPNIDKALATFRSREMSIVIVLQALDQLKTMYKNGWATLVNTCDSFLFLGGDEKETTEYLSK
RAGKQTISLKKTSVSRGRQGSSSESKDKTGRDLLTPDEIGRLKGTDALLFISGQFVYKDKKYTVHEHEQA
HLLSNTPGDANWFTYKRYRNEEEELLDKVRKEDIVDHGVITDEAA

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (139-557)

Protein structure

No available structure.

Host bacterium

• ID: 5941
• GenBank: NZ_CP016846
• Plasmid name: pL21581-2
• Incompatibility group: -
• Plasmid size: 81683 bp
• Coordinate of oriT [Strand]: 13818..13866 [+]
• Host baterium: Carnobacterium maltaromaticum strain TMW 2.1581

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrID1