Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105472
Name   oriT_pAR8416_NDM5 in_silico
Organism   Klebsiella pneumoniae strain AR8416
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP081819 (42866..43483 [+], 618 nt)
oriT length   618 nt
IRs (inverted repeats)      589..594, 608..613  (TCCATG..CATGGA)
 592..597, 602..607  (ATGCCC..GGGCAT)
 520..527, 530..537  (CCTCCCGT..ACGGGAGG)
 455..460, 464..469  (ATTCGC..GCGAAT)
 197..202, 208..213  (TTCAAT..ATTGAA)
 85..90, 96..101  (TGCTTT..AAAGCA)
Location of nic site      315..316
Conserved sequence flanking the
  nic site    		  TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 618 nt

>oriT_pAR8416_NDM5
TTCTTTGTTTCCCTGTCAAATACATTGTGGTTTTGATAAAATCATCATTGTCTTTTGACACATTAACATCTTCATTTTTAACCTTGCTTTTCAGTAAAGCATTGGCAAGCAGCTTCATTCCTTTTGCCACGGTTAAACCCGGTTCATCTGGATAAAGTGTTTTAAGACAATCAAGTAGATCATCATGTTGGCTTTCTTCAATCCTGAATTGAACTTTTTTCATAAAACTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAACTACCTCCACACATAAATTGTGCTTCAGCGTCCATCGAAATGCAAAAAATGAAAGGCTATCGTGGCTGTGATCTGGTGCGTCTGCGACCGGAGACAACGGATTCGCGGCTAACTTGGGCGGAAATAAATTCGCTACGCGAATGGCCTGGCAGGGGGCGCGAGCGCTGTTTTACGGAATATACAAAAAAAGCACCTCCCGTAAACGGGAGGGCTTCGGCGATTCAGGAACGGGAATTTTATTCTGCCTCTGGTGTGTCGCCTTCCATGCCCTGCCGGGCATCATGGAGCCAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3729 GenBank   WP_004199101
Name   mobP1_K5O16_RS27200_pAR8416_NDM5 insolico UniProt ID   A0A515HL38
Length   386 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 386 a.a.        Molecular weight: 44388.82 Da        Isoelectric Point: 10.6017

>WP_004199101.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMIDRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMNKKYPNHRFVLGYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYD
VKATHKQQHGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYWGADF
GELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGVKGIDRISSSSKELVLN
SAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(90-240)


  Protein structure


Source ID Structure
AlphaFold DB A0A515HL38


T4CP

ID   3720 GenBank   WP_000053822
Name   t4cp2_K5O16_RS27130_pAR8416_NDM5 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 69204.04 Da        Isoelectric Point: 7.4342

>WP_000053822.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLF
YIDLKEESGAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKPVFSI
GSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYITTKSTGSSKTSGRSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPY
FMDRLMMVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(102-572)

  Protein structure



No available structure.



ID   3721 GenBank   WP_015060057
Name   t4cp2_K5O16_RS27175_pAR8416_NDM5 insolico UniProt ID   _
Length   917 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 917 a.a.        Molecular weight: 104788.72 Da        Isoelectric Point: 6.2666

>WP_015060057.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYALILPGWYAIKRVTKIDERFFDLLYLR
MQIKGNPLANKRFNAVHYAGSSYDAVDISKVDNFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQ
IDGAYFECVDEADLALLTDQLNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSA
AEYFENKLYLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLYEENN
IVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARYFRCIEIKDYFQETDAG
IFDALMYLPVEYVQTSSLTPIDKQSAIKALDDQIDKLEMTDDAAKSLLADLKVGLDMVSSGYISFGKSHQ
TLIVYADSPERLVKDTNIVTTTLEDLGLIVTYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFH
NFFTGKEKGNTWGNNLITLGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFA
AQQFGTPESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNISFMKDL
VRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTETRRHGLKARLNAWVQGGEF
GWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYLIYRVTMLADGRRLLIYMDEFWQWINNEAFR
DFVYNKLKTARKLDMVLVVATQSPDELIKSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKI
KAIDPLSRQFLVVKNPQRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTY
LERANLI

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(272-474)

Type IV secretory pathway, VirB3-like protein (VirB3)

(2-83)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30176..40285

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS27105 25299..26561 - 1263 WP_000517490 ATP-binding protein -
K5O16_RS27110 26644..27138 - 495 WP_001215543 thermonuclease family protein -
K5O16_RS27115 27135..27461 - 327 WP_004208538 hypothetical protein -
K5O16_RS27120 27547..27861 - 315 WP_000754496 TrbM/KikA/MpfK family conjugal transfer protein -
K5O16_RS27125 27949..28341 - 393 WP_000738524 cag pathogenicity island Cag12 family protein -
K5O16_RS27130 28338..30173 - 1836 WP_000053822 type IV secretory system conjugative DNA transfer family protein -
K5O16_RS27135 30176..31210 - 1035 WP_000650512 P-type DNA transfer ATPase VirB11 virB11
K5O16_RS27140 31207..31404 - 198 WP_000629109 DNA-binding protein -
K5O16_RS27145 31406..32620 - 1215 WP_001295060 VirB10/TraB/TrbI family type IV secretion system protein virB10
K5O16_RS27150 32617..33546 - 930 WP_000783386 TrbG/VirB9 family P-type conjugative transfer protein virB9
K5O16_RS27155 33552..34280 - 729 WP_004199085 type IV secretion system protein virB8
K5O16_RS27160 34475..35530 - 1056 WP_000235886 type IV secretion system protein virB6
K5O16_RS27165 35542..35799 - 258 WP_000759841 EexN family lipoprotein -
K5O16_RS27170 35809..36579 - 771 WP_000716326 type IV secretion system protein -
K5O16_RS27175 36589..39342 - 2754 WP_015060057 VirB3 family type IV secretion system protein virb4
K5O16_RS27180 39367..39657 - 291 WP_000916156 TrbC/VirB2 family protein virB2
K5O16_RS27185 39641..40285 - 645 WP_000953538 lytic transglycosylase domain-containing protein virB1
K5O16_RS27190 40331..40567 - 237 WP_001348696 hypothetical protein -
K5O16_RS27195 40518..41027 - 510 WP_000455437 transcription termination/antitermination NusG family protein -
K5O16_RS27200 41380..42540 - 1161 WP_004199101 MobP1 family relaxase -
K5O16_RS27205 42543..43088 - 546 WP_001348698 DNA distortion polypeptide 1 -
K5O16_RS27210 43429..43686 - 258 WP_004199355 hypothetical protein -
K5O16_RS27215 43683..43847 - 165 WP_000004083 hypothetical protein -
K5O16_RS27220 43922..44260 - 339 WP_001099725 hypothetical protein -
K5O16_RS27225 44354..44596 - 243 WP_000008707 hypothetical protein -
K5O16_RS27230 44586..44855 - 270 WP_001006717 hypothetical protein -

Host bacterium

ID   5910 GenBank   NZ_CP081819
Plasmid name   pAR8416_NDM5 Incompatibility group   IncX3
Plasmid size   45253 bp Coordinate of oriT [Strand]   42866..43483 [+]
Host baterium   Klebsiella pneumoniae strain AR8416

Cargo genes

Drug resistance gene   blaNDM-5
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -