Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105470
• Name: oriT_AR8416|p3
• Organism: Klebsiella pneumoniae strain AR8416
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP081817 (17267..17366 [-], 100 nt)
• oriT length: 100 nt
• IRs (inverted repeats): 25..32, 35..42 (TAACCTGT..ACAGGTTA)
• Location of nic site: 61..62
• Conserved sequence flanking the nic site: TCCTGCCCGG
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 100 nt

>oriT_AR8416|p3
GAATAAGGGGAGAGTAAAGTTTGATAACCTGTTTACAGGTTAGCTAACTTTACATGTCCTGCCCGGCTTTCGCCGGTGTTTACGCCAAGGAAAGTCTACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_K5O16_RS26500_AR8416|p3

• ID: 3727
• GenBank: WP_048207647
• Name: Relaxase_K5O16_RS26500_AR8416|p3
• UniProt ID: _
• Length: 877 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 877 a.a.
• Molecular weight: 97799.96 Da
• Isoelectric Point: 10.5462

>WP_048207647.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MISRHIPMRNVKKSSFVELAKYLQDPQNKQERVGVIKVTNCHQENILDAARYDIEPTQRRNTRSEGDKTY
HLVISFRPGEKPSQEVLDAAEERICAAMGYADHQRVSAVHYDTDNVHIHIAINKIHPQTHNIHTPYNDYK
TRSAVCLALEKEFGLESDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHQAMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSVPDTKPSRPSPEAIKQRFGSAAHV
KRPGDRPPPMRENRQVNLGDVPVMQINSGKRYEGKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKK
AAREREYIHIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDA
GRERSGNGNATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPN
GERGHSKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR

Protein domains

Predicted by InterproScan.

• Large polyvalent protein-associated domain 7 (LPD7) (471-555)

• Relaxase/Mobilisation nuclease domain (Relaxase) (12-251)

• Domain of unknown function (DUF5710) (DUF5710) (565-606)

Protein structure

No available structure.

T4CP

t4cp2_K5O16_RS26510_AR8416|p3

• ID: 3718
• GenBank: WP_032310210
• Name: t4cp2_K5O16_RS26510_AR8416|p3
• UniProt ID: _
• Length: 648 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 648 a.a.
• Molecular weight: 71711.82 Da
• Isoelectric Point: 8.3842

>WP_032310210.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (119-418)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (446-629)

Protein structure

No available structure.

Host bacterium

• ID: 5908
• GenBank: NZ_CP081817
• Plasmid name: AR8416|p3
• Incompatibility group: IncP1
• Plasmid size: 45511 bp
• Coordinate of oriT [Strand]: 17267..17366 [-]
• Host baterium: Klebsiella pneumoniae strain AR8416

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -