Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105460
Name   oriT_pEho8-2 in_silico
Organism   Enterobacter hormaechei strain Eho-8
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP066103 (55911..55996 [-], 86 nt)
oriT length   86 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 86 nt

>oriT_pEho8-2
GGGTGTCGGGGCGCAGCCCTGACCAGGGTATTTGTGATGCCGGCGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3721 GenBank   WP_022650008
Name   Relaxase_GVI69_RS23925_pEho8-2 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   3710 GenBank   WP_022650006
Name   trbC_GVI69_RS23940_pEho8-2 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62119..96563

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS23930 (GVI69_23840) 59135..59338 - 204 WP_123839396 hypothetical protein -
GVI69_RS23935 (GVI69_23845) 59472..59741 - 270 WP_022650007 hypothetical protein -
GVI69_RS23940 (GVI69_23850) 59815..62115 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
GVI69_RS23945 (GVI69_23855) 62119..63375 - 1257 WP_126530067 hypothetical protein trbB
GVI69_RS23950 (GVI69_23860) 63356..64564 - 1209 WP_022650004 hypothetical protein trbA
GVI69_RS23955 (GVI69_23865) 64885..65517 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
GVI69_RS23960 (GVI69_23870) 65579..67738 - 2160 WP_022650002 DotA/TraY family protein traY
GVI69_RS23965 (GVI69_23875) 67796..68338 - 543 WP_022650001 conjugal transfer protein TraX -
GVI69_RS23970 (GVI69_23880) 68335..69537 - 1203 WP_022650000 conjugal transfer protein TraW traW
GVI69_RS23975 (GVI69_23885) 69550..70122 - 573 WP_154590113 conjugal transfer protein TraV traV
GVI69_RS23980 (GVI69_23890) 70119..73172 - 3054 WP_022649998 hypothetical protein traU
GVI69_RS23985 (GVI69_23895) 73259..73984 - 726 WP_063864836 hypothetical protein traT
GVI69_RS23990 (GVI69_23900) 74168..74572 - 405 WP_022649996 DUF6750 family protein traR
GVI69_RS23995 (GVI69_23905) 74607..75131 - 525 WP_022649995 conjugal transfer protein TraQ traQ
GVI69_RS24000 (GVI69_23910) 75131..75904 - 774 WP_123839398 conjugal transfer protein TraP traP
GVI69_RS24005 (GVI69_23915) 75885..77192 - 1308 WP_022649993 conjugal transfer protein TraO traO
GVI69_RS24010 (GVI69_23920) 77192..78172 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
GVI69_RS24015 (GVI69_23925) 78183..78800 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
GVI69_RS24020 (GVI69_23930) 78890..79240 - 351 WP_022649990 hypothetical protein traL
GVI69_RS24025 (GVI69_23935) 79258..82800 - 3543 WP_032635082 LPD7 domain-containing protein -
GVI69_RS24030 (GVI69_23940) 82872..83159 - 288 WP_022649988 hypothetical protein traK
GVI69_RS24035 (GVI69_23945) 83156..84316 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
GVI69_RS24040 (GVI69_23950) 84329..85135 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
GVI69_RS24045 (GVI69_23955) 85132..85605 - 474 WP_022649985 DotD/TraH family lipoprotein -
GVI69_RS24050 (GVI69_23960) 85676..86092 - 417 WP_123839394 hypothetical protein -
GVI69_RS24055 (GVI69_23965) 86201..87406 - 1206 WP_022649984 conjugal transfer protein TraF -
GVI69_RS24060 (GVI69_23970) 87512..88348 - 837 WP_022649983 hypothetical protein traE
GVI69_RS24065 (GVI69_23975) 88495..88971 - 477 WP_022649982 hypothetical protein -
GVI69_RS24070 (GVI69_23980) 88991..90151 - 1161 WP_022649981 site-specific integrase -
GVI69_RS24075 (GVI69_23985) 90732..92066 - 1335 WP_045346679 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
GVI69_RS24080 (GVI69_23990) 92063..92737 - 675 WP_022649979 prepilin peptidase -
GVI69_RS24085 (GVI69_23995) 92722..93204 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
GVI69_RS24090 (GVI69_24000) 93289..93903 - 615 WP_022649977 type 4 pilus major pilin -
GVI69_RS24095 (GVI69_24005) 93917..95011 - 1095 WP_032623575 type II secretion system F family protein -
GVI69_RS24100 (GVI69_24010) 95004..96563 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
GVI69_RS24105 (GVI69_24015) 96566..97081 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
GVI69_RS24110 (GVI69_24020) 97065..98369 - 1305 WP_022649973 type 4b pilus protein PilO2 -
GVI69_RS24115 (GVI69_24025) 98371..100047 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
GVI69_RS24120 (GVI69_24030) 100062..100499 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   5898 GenBank   NZ_CP066103
Plasmid name   pEho8-2 Incompatibility group   -
Plasmid size   106698 bp Coordinate of oriT [Strand]   55911..55996 [-]
Host baterium   Enterobacter hormaechei strain Eho-8

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7