Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105441 |
| Name | oriT1_pM297-1.2  |
| Organism | Klebsiella pneumoniae strain M297-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP051492 ( 216997..217045 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT1_pM297-1.2
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3697 | GenBank | WP_228294104 |
| Name | traD_HGK25_RS27180_pM297-1.2 |
UniProt ID | _ |
| Length | 768 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 768 a.a. Molecular weight: 85788.91 Da Isoelectric Point: 5.1149
>WP_228294104.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRKQQS
EDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVRAR
GDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQGSG
RTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKAIR
YLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWI
FADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIAEF
AAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLALKY
KPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPAPA
DMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTEQE
LAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRKQQS
EDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVRAR
GDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQGSG
RTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKAIR
YLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWI
FADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIAEF
AAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLALKY
KPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPAPA
DMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTEQE
LAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48080..76839
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HGK25_RS27180 (HGK25_27720) | 48080..50386 | - | 2307 | WP_228294104 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HGK25_RS28590 | 50513..51200 | - | 688 | Protein_52 | hypothetical protein | - |
| HGK25_RS27190 (HGK25_27730) | 51389..52120 | - | 732 | WP_013023830 | conjugal transfer complement resistance protein TraT | - |
| HGK25_RS27195 (HGK25_27735) | 52306..52839 | - | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| HGK25_RS27200 (HGK25_27740) | 52842..55686 | - | 2845 | Protein_55 | conjugal transfer mating-pair stabilization protein TraG | - |
| HGK25_RS27205 (HGK25_27745) | 55686..57055 | - | 1370 | Protein_56 | conjugal transfer pilus assembly protein TraH | - |
| HGK25_RS27210 (HGK25_27750) | 57033..57472 | - | 440 | Protein_57 | F-type conjugal transfer protein TrbF | - |
| HGK25_RS27215 (HGK25_27755) | 57518..58075 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HGK25_RS27220 (HGK25_27760) | 58047..58285 | - | 239 | Protein_59 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HGK25_RS27225 (HGK25_27765) | 58296..59047 | - | 752 | Protein_60 | type-F conjugative transfer system pilin assembly protein TraF | - |
| HGK25_RS27230 (HGK25_27770) | 59067..59355 | - | 289 | Protein_61 | hypothetical protein | - |
| HGK25_RS27235 (HGK25_27775) | 59396..59643 | - | 248 | Protein_62 | hypothetical protein | - |
| HGK25_RS27240 (HGK25_27780) | 59621..59875 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| HGK25_RS28765 (HGK25_27785) | 59907..61852 | - | 1946 | Protein_64 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| HGK25_RS27250 (HGK25_27790) | 61911..62549 | - | 639 | WP_011977786 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HGK25_RS27255 (HGK25_27795) | 62562..63551 | - | 990 | WP_009309872 | conjugal transfer pilus assembly protein TraU | traU |
| HGK25_RS27260 (HGK25_27800) | 63548..63937 | - | 390 | WP_004194992 | hypothetical protein | - |
| HGK25_RS27265 (HGK25_27805) | 63979..64605 | - | 627 | WP_009309871 | type-F conjugative transfer system protein TraW | traW |
| HGK25_RS27270 (HGK25_27810) | 64605..64994 | - | 390 | WP_004197815 | type-F conjugative transfer system protein TrbI | - |
| HGK25_RS27275 (HGK25_27815) | 64994..67633 | - | 2640 | WP_013023824 | type IV secretion system protein TraC | virb4 |
| HGK25_RS27280 (HGK25_27820) | 67705..68103 | - | 399 | WP_013609531 | hypothetical protein | - |
| HGK25_RS27285 (HGK25_27825) | 68479..68883 | - | 405 | WP_004197817 | hypothetical protein | - |
| HGK25_RS27290 (HGK25_27830) | 68950..69261 | - | 312 | WP_015344986 | hypothetical protein | - |
| HGK25_RS27295 (HGK25_27835) | 69262..69479 | - | 218 | Protein_74 | hypothetical protein | - |
| HGK25_RS27300 (HGK25_27840) | 69585..69995 | - | 411 | WP_009309869 | hypothetical protein | - |
| HGK25_RS27305 (HGK25_27845) | 70127..70711 | - | 585 | WP_013023822 | type IV conjugative transfer system lipoprotein TraV | traV |
| HGK25_RS27310 (HGK25_27850) | 70825..72248 | - | 1424 | Protein_77 | F-type conjugal transfer pilus assembly protein TraB | - |
| HGK25_RS27315 (HGK25_27855) | 72248..72988 | - | 741 | WP_013023821 | type-F conjugative transfer system secretin TraK | traK |
| HGK25_RS27320 (HGK25_27860) | 72975..73540 | - | 566 | Protein_79 | type IV conjugative transfer system protein TraE | - |
| HGK25_RS27325 (HGK25_27865) | 73560..73863 | - | 304 | Protein_80 | type IV conjugative transfer system protein TraL | - |
| HGK25_RS27330 (HGK25_27870) | 73877..74245 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| HGK25_RS27335 (HGK25_27875) | 74314..74514 | - | 201 | WP_004194116 | TraY domain-containing protein | - |
| HGK25_RS27340 (HGK25_27880) | 74600..75301 | - | 702 | WP_004194113 | hypothetical protein | - |
| HGK25_RS27345 (HGK25_27885) | 75531..75923 | - | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HGK25_RS27350 (HGK25_27890) | 76354..76839 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| HGK25_RS27355 (HGK25_27895) | 76872..77201 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| HGK25_RS27360 (HGK25_27900) | 77234..78051 | - | 818 | Protein_87 | DUF932 domain-containing protein | - |
| HGK25_RS27365 (HGK25_27910) | 78859..80067 | + | 1209 | WP_015344990 | IS256 family transposase | - |
| HGK25_RS27370 (HGK25_27920) | 80203..80616 | - | 414 | WP_013023817 | helix-turn-helix domain-containing protein | - |
| HGK25_RS27375 (HGK25_27925) | 80617..80895 | - | 279 | WP_196542349 | helix-turn-helix transcriptional regulator | - |
| HGK25_RS27380 (HGK25_27930) | 80885..81203 | - | 319 | Protein_91 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HGK25_RS27385 (HGK25_27935) | 81284..81508 | - | 225 | WP_004152719 | hypothetical protein | - |
| HGK25_RS27390 (HGK25_27940) | 81519..81731 | - | 213 | WP_013023815 | hypothetical protein | - |
Host bacterium
| ID | 5879 | GenBank | NZ_CP051492 |
| Plasmid name | pM297-1.2 | Incompatibility group | IncFII |
| Plasmid size | 225763 bp | Coordinate of oriT [Strand] | 76234..76282 [+]; 216997..217045 [+] |
| Host baterium | Klebsiella pneumoniae strain M297-1 |
Cargo genes
| Drug resistance gene | blaCTX-M-14, blaLAP-2, sul2, aph(3'')-Ib, aph(6)-Id, aph(3')-Ia, aac(3)-IId, floR, tet(A), mph(A), sul1, qnrB91, qacE, aadA16, dfrA27, ARR-3, aac(6')-Ib-cr, blaTEM-1B, blaCTX-M-3, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |