Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105437
Name   oriT_pSA-KpST14-3 in_silico
Organism   Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071282 (71654..71703 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pSA-KpST14-3
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3707 GenBank   WP_043907038
Name   traI_JZ794_RS28295_pSA-KpST14-3 insolico UniProt ID   A0A0S1TKY9
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190458.19 Da        Isoelectric Point: 6.0263

>WP_043907038.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSLSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

AAA domain (AAA_30)

(969-1156)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




T4CP

ID   3694 GenBank   WP_043907039
Name   traD_JZ794_RS28290_pSA-KpST14-3 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85906.85 Da        Isoelectric Point: 5.0577

>WP_043907039.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 71096..100260

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZ794_RS28070 (JZ794_28075) 66113..66463 + 351 WP_004152758 hypothetical protein -
JZ794_RS28075 (JZ794_28080) 67099..67455 + 357 WP_004152717 hypothetical protein -
JZ794_RS28080 (JZ794_28085) 67516..67728 + 213 WP_004152718 hypothetical protein -
JZ794_RS28085 (JZ794_28090) 67739..67963 + 225 WP_004152719 hypothetical protein -
JZ794_RS28090 (JZ794_28095) 68044..68364 + 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
JZ794_RS28095 (JZ794_28100) 68354..68632 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
JZ794_RS28100 (JZ794_28105) 68633..69046 + 414 WP_004152722 helix-turn-helix domain-containing protein -
JZ794_RS28105 (JZ794_28110) 69880..70701 + 822 WP_004178064 DUF932 domain-containing protein -
JZ794_RS28110 (JZ794_28115) 70734..71063 + 330 WP_011977736 DUF5983 family protein -
JZ794_RS28115 (JZ794_28120) 71096..71581 - 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
JZ794_RS28120 (JZ794_28125) 72015..72407 + 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
JZ794_RS28125 (JZ794_28130) 72621..73316 + 696 WP_004178061 transcriptional regulator TraJ family protein -
JZ794_RS28130 (JZ794_28135) 73400..73771 + 372 WP_004208838 TraY domain-containing protein -
JZ794_RS28135 (JZ794_28140) 73825..74193 + 369 WP_004152496 type IV conjugative transfer system pilin TraA -
JZ794_RS28140 (JZ794_28145) 74207..74512 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
JZ794_RS28145 (JZ794_28150) 74532..75098 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
JZ794_RS28150 (JZ794_28155) 75085..75825 + 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
JZ794_RS28155 (JZ794_28160) 75825..77249 + 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
JZ794_RS28160 (JZ794_28165) 77242..77838 + 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
JZ794_RS28165 (JZ794_28170) 77861..78430 + 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
JZ794_RS28170 (JZ794_28175) 78562..78972 + 411 WP_004152597 lipase chaperone -
JZ794_RS28175 (JZ794_28180) 78977..79267 + 291 WP_004152596 hypothetical protein -
JZ794_RS28180 (JZ794_28185) 79291..79509 + 219 WP_004171484 hypothetical protein -
JZ794_RS28185 (JZ794_28190) 79510..79827 + 318 WP_004152595 hypothetical protein -
JZ794_RS28190 (JZ794_28195) 79894..80298 + 405 WP_004152594 hypothetical protein -
JZ794_RS28195 (JZ794_28200) 80594..80992 + 399 WP_004153071 hypothetical protein -
JZ794_RS28200 (JZ794_28205) 81064..83703 + 2640 WP_004152592 type IV secretion system protein TraC virb4
JZ794_RS28205 (JZ794_28210) 83703..84081 + 379 Protein_102 type-F conjugative transfer system protein TrbI -
JZ794_RS28210 (JZ794_28215) 84081..84707 + 627 WP_004152590 type-F conjugative transfer system protein TraW traW
JZ794_RS28215 (JZ794_28220) 84751..85710 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
JZ794_RS28220 (JZ794_28225) 85723..86361 + 639 WP_004152682 type-F conjugative transfer system pilin assembly protein TrbC trbC
JZ794_RS28225 (JZ794_28230) 86409..88364 + 1956 WP_040145764 type-F conjugative transfer system mating-pair stabilization protein TraN traN
JZ794_RS28230 (JZ794_28235) 88396..88650 + 255 WP_004152674 conjugal transfer protein TrbE -
JZ794_RS28235 (JZ794_28240) 88628..88876 + 249 WP_013214029 hypothetical protein -
JZ794_RS28240 (JZ794_28245) 88889..89215 + 327 WP_013214030 hypothetical protein -
JZ794_RS28245 (JZ794_28250) 89236..89988 + 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
JZ794_RS28250 (JZ794_28255) 89999..90238 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
JZ794_RS28255 (JZ794_28260) 90210..90767 + 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
JZ794_RS28260 (JZ794_28265) 90813..91256 + 444 WP_030003485 F-type conjugal transfer protein TrbF -
JZ794_RS28265 (JZ794_28270) 91234..92613 + 1380 WP_011977731 conjugal transfer pilus assembly protein TraH traH
JZ794_RS28270 (JZ794_28275) 92613..95462 + 2850 WP_004152624 conjugal transfer mating-pair stabilization protein TraG traG
JZ794_RS28275 (JZ794_28280) 95475..96023 + 549 WP_004152623 conjugal transfer entry exclusion protein TraS -
JZ794_RS28280 (JZ794_28285) 96207..96938 + 732 WP_004152622 conjugal transfer complement resistance protein TraT -
JZ794_RS28285 (JZ794_28290) 97130..97819 + 690 WP_004198206 hypothetical protein -
JZ794_RS28290 (JZ794_28295) 97948..100260 + 2313 WP_043907039 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   5875 GenBank   NZ_CP071282
Plasmid name   pSA-KpST14-3 Incompatibility group   IncFIB
Plasmid size   166565 bp Coordinate of oriT [Strand]   71654..71703 [-]
Host baterium   Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoE, arsH, arsC, arsB, arsA, arsD, arsR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9