Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105436 |
| Name | oriT_pSA-KpST14-OXA48-2  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP071281 (45578..45682 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pSA-KpST14-OXA48-2
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3706 | GenBank | WP_004206924 |
| Name | Relaxase_JZ794_RS27595_pSA-KpST14-OXA48-2 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 3693 | GenBank | WP_004206885 |
| Name | t4cp2_JZ794_RS27300_pSA-KpST14-OXA48-2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48480..67676
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JZ794_RS27575 (JZ794_27580) | 43564..43875 | + | 312 | WP_004187333 | hypothetical protein | - |
| JZ794_RS27580 (JZ794_27585) | 44008..44544 | + | 537 | WP_036970776 | hypothetical protein | - |
| JZ794_RS27585 (JZ794_27590) | 45045..45410 | - | 366 | WP_015586046 | hypothetical protein | - |
| JZ794_RS27590 (JZ794_27595) | 45686..46003 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| JZ794_RS27595 (JZ794_27600) | 45990..47969 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| JZ794_RS27600 (JZ794_27605) | 47983..48483 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| JZ794_RS27605 (JZ794_27610) | 48480..49259 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| JZ794_RS27610 (JZ794_27615) | 49270..50433 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| JZ794_RS27615 (JZ794_27620) | 50423..50683 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| JZ794_RS28970 | 50708..54013 | + | 3306 | WP_220381329 | LPD7 domain-containing protein | - |
| JZ794_RS27625 (JZ794_27630) | 53979..54491 | + | 513 | WP_011091071 | hypothetical protein | traL |
| JZ794_RS27630 (JZ794_27635) | 54492..54704 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| JZ794_RS27635 (JZ794_27640) | 54676..55086 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| JZ794_RS27640 (JZ794_27645) | 55154..55867 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| JZ794_RS27645 (JZ794_27650) | 55876..57027 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| JZ794_RS27650 (JZ794_27655) | 57039..58388 | + | 1350 | WP_036970781 | conjugal transfer protein TraO | traO |
| JZ794_RS27655 (JZ794_27660) | 58400..59104 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| JZ794_RS27660 (JZ794_27665) | 59128..59658 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| JZ794_RS27665 (JZ794_27670) | 59675..60064 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| JZ794_RS27670 (JZ794_27675) | 60110..60604 | + | 495 | WP_004187480 | hypothetical protein | - |
| JZ794_RS27675 (JZ794_27680) | 60601..63651 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| JZ794_RS27680 (JZ794_27685) | 63648..64856 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| JZ794_RS27685 (JZ794_27690) | 64853..65503 | + | 651 | WP_004187488 | hypothetical protein | - |
| JZ794_RS27690 (JZ794_27695) | 65496..67676 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| JZ794_RS27695 (JZ794_27700) | 67679..68332 | + | 654 | WP_004206935 | hypothetical protein | - |
| JZ794_RS27700 (JZ794_27705) | 68406..68636 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 5874 | GenBank | NZ_CP071281 |
| Plasmid name | pSA-KpST14-OXA48-2 | Incompatibility group | IncL/M |
| Plasmid size | 68932 bp | Coordinate of oriT [Strand] | 45578..45682 [+] |
| Host baterium | Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14 |
Cargo genes
| Drug resistance gene | blaOXA-48, blaCTX-M-14b, aph(3'')-Ib, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |