Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105431 |
| Name | oriT_CriePir116|unnamed  |
| Organism | Klebsiella pneumoniae strain CriePir116 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP063012 (141016..141121 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_CriePir116|unnamed
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3702 | GenBank | WP_050868819 |
| Name | Relaxase_GJI97_RS27365_CriePir116|unnamed |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 3690 | GenBank | WP_011091092 |
| Name | t4cp2_GJI97_RS27075_CriePir116|unnamed |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 143917..163020
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GJI97_RS27345 (GJI97_027345) | 139001..139312 | + | 312 | WP_011091065 | hypothetical protein | - |
| GJI97_RS27350 (GJI97_027350) | 139445..139981 | + | 537 | WP_011091066 | hypothetical protein | - |
| GJI97_RS27355 (GJI97_027355) | 140482..140847 | - | 366 | WP_011091067 | hypothetical protein | - |
| GJI97_RS27695 | 140862..141440 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| GJI97_RS27365 (GJI97_027365) | 141427..143406 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| GJI97_RS27370 (GJI97_027370) | 143420..143920 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| GJI97_RS27375 (GJI97_027375) | 143917..144696 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| GJI97_RS27380 (GJI97_027380) | 144707..145870 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| GJI97_RS27385 (GJI97_027385) | 145860..146120 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| GJI97_RS27700 | 146145..149357 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| GJI97_RS27395 (GJI97_027395) | 149323..149835 | + | 513 | WP_011091071 | hypothetical protein | traL |
| GJI97_RS27400 (GJI97_027400) | 149836..150048 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| GJI97_RS27405 (GJI97_027405) | 150020..150430 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| GJI97_RS27705 | 150498..150704 | + | 207 | WP_227659897 | hypothetical protein | traM |
| GJI97_RS27710 | 150756..151211 | + | 456 | WP_264755453 | DotI/IcmL family type IV secretion protein | traM |
| GJI97_RS27415 (GJI97_027415) | 151220..152371 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| GJI97_RS27420 (GJI97_027420) | 152383..153732 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| GJI97_RS27425 (GJI97_027425) | 153744..154448 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| GJI97_RS27430 (GJI97_027430) | 154472..155002 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| GJI97_RS27435 (GJI97_027435) | 155019..155408 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| GJI97_RS27440 (GJI97_027440) | 155454..155948 | + | 495 | WP_011091080 | hypothetical protein | - |
| GJI97_RS27445 (GJI97_027445) | 155945..158995 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| GJI97_RS27450 (GJI97_027450) | 158992..160200 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| GJI97_RS27455 (GJI97_027455) | 160197..160847 | + | 651 | WP_011091083 | hypothetical protein | - |
| GJI97_RS27460 (GJI97_027460) | 160840..163020 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| GJI97_RS27465 (GJI97_027465) | 163023..163676 | + | 654 | WP_011091084 | hypothetical protein | - |
| GJI97_RS27470 (GJI97_027470) | 163751..163981 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 5869 | GenBank | NZ_CP063012 |
| Plasmid name | CriePir116|unnamed | Incompatibility group | IncFIA |
| Plasmid size | 164278 bp | Coordinate of oriT [Strand] | 141016..141121 [+] |
| Host baterium | Klebsiella pneumoniae strain CriePir116 |
Cargo genes
| Drug resistance gene | blaTEM-1B, tet(D), aac(6')-Ib-cr, ARR-3, dfrA27, aadA16, qacE, sul1, sul2, aph(3'')-Ib, aph(6)-Id, blaCTX-M-15 |
| Virulence gene | - |
| Metal resistance gene | merR, merT, merP, merC, merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |