Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105423
Name   oriT_p30148-1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Worthington strain CVM 30148
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP051354 (48966..49052 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_p30148-1
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3696 GenBank   WP_001354015
Name   nikB_HFS90_RS24505_p30148-1 insolico UniProt ID   E8XLT1
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104018.47 Da        Isoelectric Point: 7.3526

>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E8XLT1


Auxiliary protein

ID   1670 GenBank   WP_001281051
Name   WP_001281051_p30148-1 insolico UniProt ID   A0A3U8I0C6
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12646.44 Da        Isoelectric Point: 10.4030

>WP_001281051.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A3U8I0C6


T4CP

ID   3683 GenBank   WP_001289276
Name   trbC_HFS90_RS24510_p30148-1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54431..92730

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFS90_RS24510 (HFS90_24515) 52147..54438 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HFS90_RS24515 (HFS90_24520) 54431..55501 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HFS90_RS24520 (HFS90_24525) 55520..56728 - 1209 WP_000121274 IncI1-type conjugal transfer protein TrbA trbA
HFS90_RS24525 (HFS90_24530) 57020..57172 + 153 WP_001331364 Hok/Gef family protein -
HFS90_RS25300 57244..57435 - 192 WP_174715448 hypothetical protein -
HFS90_RS25235 57996..58091 + 96 WP_000609148 DinQ-like type I toxin DqlB -
HFS90_RS24535 (HFS90_24540) 58156..58332 - 177 WP_001054904 hypothetical protein -
HFS90_RS24540 (HFS90_24545) 58724..58933 + 210 WP_000062603 HEAT repeat domain-containing protein -
HFS90_RS24545 (HFS90_24550) 59005..59667 - 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
HFS90_RS24550 (HFS90_24555) 59732..61894 - 2163 WP_000698351 DotA/TraY family protein traY
HFS90_RS24555 (HFS90_24560) 61991..62575 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HFS90_RS24560 (HFS90_24565) 62604..63806 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HFS90_RS24565 (HFS90_24570) 63773..64387 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HFS90_RS24570 (HFS90_24575) 64387..67431 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
HFS90_RS24575 (HFS90_24580) 67521..68321 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HFS90_RS24580 (HFS90_24585) 68305..68493 - 189 WP_001277255 putative conjugal transfer protein TraS -
HFS90_RS24585 (HFS90_24590) 68557..68961 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
HFS90_RS24590 (HFS90_24595) 69012..69539 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HFS90_RS24595 (HFS90_24600) 69539..70243 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HFS90_RS24600 (HFS90_24605) 70243..71532 - 1290 WP_001272005 conjugal transfer protein TraO traO
HFS90_RS24605 (HFS90_24610) 71535..72518 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HFS90_RS24610 (HFS90_24615) 72529..73221 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
HFS90_RS24615 (HFS90_24620) 73218..73565 - 348 WP_001055900 conjugal transfer protein traL
HFS90_RS24620 (HFS90_24625) 73583..77350 - 3768 WP_001141529 LPD7 domain-containing protein -
HFS90_RS24625 (HFS90_24630) 77440..77991 - 552 WP_000014583 phospholipase D family protein -
HFS90_RS24630 (HFS90_24635) 78006..78296 - 291 WP_001299214 hypothetical protein traK
HFS90_RS24635 (HFS90_24640) 78293..79441 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HFS90_RS24640 (HFS90_24645) 79438..80256 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HFS90_RS24645 (HFS90_24650) 80253..80711 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HFS90_RS24650 (HFS90_24655) 81106..81690 - 585 WP_000977522 histidine phosphatase family protein -
HFS90_RS24655 (HFS90_24660) 81750..82952 - 1203 WP_000976351 conjugal transfer protein TraF -
HFS90_RS24660 (HFS90_24665) 83037..83861 - 825 WP_001238939 conjugal transfer protein TraE traE
HFS90_RS24665 (HFS90_24670) 84012..85166 - 1155 WP_001139957 site-specific integrase -
HFS90_RS24670 (HFS90_24675) 86687..86935 + 249 WP_001349157 hypothetical protein -
HFS90_RS24675 (HFS90_24680) 86932..88224 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HFS90_RS24680 (HFS90_24685) 88224..88880 - 657 WP_001193553 prepilin peptidase -
HFS90_RS24685 (HFS90_24690) 88865..89425 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HFS90_RS24690 (HFS90_24695) 89435..90049 - 615 WP_000959785 type 4 pilus major pilin -
HFS90_RS24695 (HFS90_24700) 90067..91164 - 1098 WP_001208805 type II secretion system F family protein -
HFS90_RS24700 (HFS90_24705) 91177..92730 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HFS90_RS24705 (HFS90_24710) 92741..93193 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
HFS90_RS24710 (HFS90_24715) 93180..94475 - 1296 WP_000752774 type 4b pilus protein PilO2 -
HFS90_RS24715 (HFS90_24720) 94468..96150 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HFS90_RS24720 (HFS90_24725) 96164..96601 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HFS90_RS24725 (HFS90_24730) 96601..97668 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   5861 GenBank   NZ_CP051354
Plasmid name   p30148-1 Incompatibility group   IncI1
Plasmid size   104600 bp Coordinate of oriT [Strand]   48966..49052 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Worthington strain CVM 30148

Cargo genes

Drug resistance gene   sul3, ant(3'')-Ia, cmlA1, aadA2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -