Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105419 |
| Name | oriT_p34514-1  |
| Organism | Salmonella enterica subsp. enterica serovar Senftenberg strain CVM 34514 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP051327 (1607..1659 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p34514-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3682 | GenBank | WP_044722407 |
| Name | t4cp2_HFT73_RS00285_p34514-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73374.92 Da Isoelectric Point: 9.3476
>WP_044722407.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19767..42695
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HFT73_RS00150 (HFT73_00170) | 15850..16302 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| HFT73_RS00155 (HFT73_00175) | 16295..16489 | + | 195 | WP_001127356 | DUF1187 family protein | - |
| HFT73_RS00160 (HFT73_00180) | 16535..17488 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HFT73_RS00165 (HFT73_00185) | 17862..18305 | + | 444 | WP_000964330 | NfeD family protein | - |
| HFT73_RS00170 (HFT73_00190) | 18309..18479 | + | 171 | WP_000550720 | hypothetical protein | - |
| HFT73_RS00175 (HFT73_00195) | 18490..18936 | + | 447 | WP_001243165 | hypothetical protein | - |
| HFT73_RS00180 (HFT73_00200) | 18969..19226 | + | 258 | WP_001542015 | hypothetical protein | - |
| HFT73_RS00185 (HFT73_00205) | 19207..19509 | + | 303 | WP_001360345 | hypothetical protein | - |
| HFT73_RS00190 (HFT73_00210) | 19506..19763 | + | 258 | WP_000739144 | hypothetical protein | - |
| HFT73_RS00195 (HFT73_00215) | 19767..20762 | + | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HFT73_RS00200 (HFT73_00220) | 20768..21409 | + | 642 | WP_001425343 | type IV secretion system protein | - |
| HFT73_RS00205 (HFT73_00225) | 21411..21665 | + | 255 | WP_001043555 | EexN family lipoprotein | - |
| HFT73_RS00210 (HFT73_00230) | 21678..21965 | + | 288 | WP_001032611 | EexN family lipoprotein | - |
| HFT73_RS00215 (HFT73_00235) | 22038..22673 | + | 636 | WP_000835773 | hypothetical protein | - |
| HFT73_RS00220 (HFT73_00245) | 22845..23132 | + | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HFT73_RS00225 (HFT73_00250) | 23135..24370 | + | 1236 | WP_000733394 | TcpQ domain-containing protein | - |
| HFT73_RS00230 (HFT73_00255) | 24376..24813 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HFT73_RS00235 (HFT73_00260) | 24932..25330 | + | 399 | WP_001153668 | hypothetical protein | - |
| HFT73_RS00240 (HFT73_00265) | 25351..25935 | + | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| HFT73_RS24855 | 25935..26225 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| HFT73_RS00250 (HFT73_00275) | 26296..26616 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HFT73_RS00255 (HFT73_00280) | 26622..28979 | + | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| HFT73_RS00260 (HFT73_00285) | 29011..29145 | + | 135 | WP_000701233 | hypothetical protein | - |
| HFT73_RS00265 (HFT73_00290) | 29145..29879 | + | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| HFT73_RS00270 (HFT73_00295) | 29945..30646 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HFT73_RS00275 (HFT73_00300) | 30636..31775 | + | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| HFT73_RS00280 (HFT73_00305) | 31794..32849 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HFT73_RS00285 (HFT73_00310) | 32865..34823 | + | 1959 | WP_044722407 | type IV secretory system conjugative DNA transfer family protein | - |
| HFT73_RS00290 (HFT73_00315) | 34870..35406 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HFT73_RS00295 (HFT73_00320) | 35399..37042 | + | 1644 | WP_199275441 | PilN family type IVB pilus formation outer membrane protein | - |
| HFT73_RS00300 (HFT73_00325) | 37093..38403 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| HFT73_RS00305 (HFT73_00330) | 38387..38881 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HFT73_RS00310 (HFT73_00335) | 38906..40444 | + | 1539 | WP_000466227 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HFT73_RS00315 (HFT73_00340) | 40435..41544 | + | 1110 | WP_000974902 | type II secretion system F family protein | - |
| HFT73_RS00320 (HFT73_00345) | 41589..42146 | + | 558 | WP_000095049 | type 4 pilus major pilin | - |
| HFT73_RS00325 (HFT73_00350) | 42213..42695 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HFT73_RS00330 (HFT73_00355) | 42699..43334 | + | 636 | WP_000934978 | A24 family peptidase | - |
| HFT73_RS00335 (HFT73_00360) | 43347..44630 | + | 1284 | WP_172690226 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFT73_RS25180 | 45270..45504 | - | 235 | Protein_62 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFT73_RS25135 | 45956..46087 | - | 132 | WP_255639686 | hypothetical protein | - |
| HFT73_RS00340 (HFT73_00365) | 46275..46721 | - | 447 | WP_199275442 | hypothetical protein | - |
Host bacterium
| ID | 5857 | GenBank | NZ_CP051327 |
| Plasmid name | p34514-1 | Incompatibility group | IncI2 |
| Plasmid size | 58790 bp | Coordinate of oriT [Strand] | 1607..1659 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Senftenberg strain CVM 34514 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |