Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105415 |
| Name | oriT_p20723-3  |
| Organism | Salmonella enterica subsp. enterica serovar Uganda strain CVM 20723 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP051424 (44166..44218 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p20723-3
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3677 | GenBank | WP_000338972 |
| Name | t4cp2_HFQ25_RS00725_p20723-3 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73329.86 Da Isoelectric Point: 9.1391
>WP_000338972.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5275..28272
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HFQ25_RS00590 (HFQ25_00600) | 767..1219 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| HFQ25_RS00595 (HFQ25_00605) | 1212..1406 | + | 195 | WP_001127358 | DUF1187 family protein | - |
| HFQ25_RS00600 (HFQ25_00610) | 1452..2405 | + | 954 | WP_072097371 | SPFH domain-containing protein | - |
| HFQ25_RS00605 (HFQ25_00615) | 2467..2910 | + | 444 | WP_000498521 | NfeD family protein | - |
| HFQ25_RS00610 (HFQ25_00620) | 2914..3084 | + | 171 | WP_000550721 | hypothetical protein | - |
| HFQ25_RS00615 (HFQ25_00625) | 3095..3757 | + | 663 | WP_001243159 | hypothetical protein | - |
| HFQ25_RS00620 (HFQ25_00630) | 3908..4501 | + | 594 | WP_001243171 | hypothetical protein | - |
| HFQ25_RS00625 (HFQ25_00635) | 4715..5017 | + | 303 | WP_000189499 | hypothetical protein | - |
| HFQ25_RS00630 (HFQ25_00640) | 5014..5271 | + | 258 | WP_000739144 | hypothetical protein | - |
| HFQ25_RS00635 (HFQ25_00645) | 5275..6270 | + | 996 | WP_000276068 | type IV secretion system protein | virB6 |
| HFQ25_RS00640 (HFQ25_00650) | 6276..6917 | + | 642 | WP_001415805 | type IV secretion system protein | - |
| HFQ25_RS00645 (HFQ25_00655) | 6920..7174 | + | 255 | WP_000609210 | EexN family lipoprotein | - |
| HFQ25_RS00650 (HFQ25_00660) | 7227..8036 | - | 810 | WP_001005154 | DUF5710 domain-containing protein | - |
| HFQ25_RS00655 (HFQ25_00665) | 8084..8719 | + | 636 | WP_000835769 | hypothetical protein | - |
| HFQ25_RS00660 (HFQ25_00675) | 8891..9178 | + | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HFQ25_RS00665 (HFQ25_00680) | 9181..10416 | + | 1236 | WP_000733393 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HFQ25_RS00670 (HFQ25_00685) | 10422..10859 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HFQ25_RS00675 (HFQ25_00690) | 10978..11376 | + | 399 | WP_001153669 | hypothetical protein | - |
| HFQ25_RS00680 (HFQ25_00695) | 11397..11981 | + | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| HFQ25_RS00685 | 11981..12271 | + | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| HFQ25_RS00690 (HFQ25_00705) | 12342..12662 | + | 321 | WP_000362083 | VirB3 family type IV secretion system protein | virB3 |
| HFQ25_RS00695 (HFQ25_00710) | 12668..15025 | + | 2358 | WP_000548951 | VirB4 family type IV secretion system protein | virb4 |
| HFQ25_RS00700 (HFQ25_00715) | 15057..15191 | + | 135 | WP_000701233 | hypothetical protein | - |
| HFQ25_RS00705 (HFQ25_00720) | 15191..15925 | + | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| HFQ25_RS00710 (HFQ25_00725) | 15991..16692 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HFQ25_RS00715 (HFQ25_00730) | 16682..17821 | + | 1140 | WP_000790639 | TrbI/VirB10 family protein | virB10 |
| HFQ25_RS00720 (HFQ25_00735) | 17896..18951 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HFQ25_RS00725 (HFQ25_00740) | 18967..20925 | + | 1959 | WP_000338972 | type IV secretory system conjugative DNA transfer family protein | - |
| HFQ25_RS00730 (HFQ25_00745) | 20976..22619 | + | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| HFQ25_RS00735 (HFQ25_00750) | 22670..23980 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| HFQ25_RS00740 (HFQ25_00755) | 23964..24458 | + | 495 | WP_000912554 | type IV pilus biogenesis protein PilP | - |
| HFQ25_RS00745 (HFQ25_00760) | 24483..26021 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HFQ25_RS00750 (HFQ25_00765) | 26012..27121 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HFQ25_RS00755 (HFQ25_00770) | 27166..27723 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HFQ25_RS00760 (HFQ25_00775) | 27790..28272 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HFQ25_RS00765 (HFQ25_00780) | 28276..28911 | + | 636 | WP_000934978 | A24 family peptidase | - |
| HFQ25_RS00770 (HFQ25_00785) | 28924..30300 | + | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFQ25_RS25130 | 30297..30518 | - | 222 | Protein_37 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFQ25_RS00780 (HFQ25_00790) | 30581..31705 | + | 1125 | WP_000486720 | site-specific integrase | - |
| HFQ25_RS00785 (HFQ25_00795) | 31717..32370 | + | 654 | WP_000572549 | hypothetical protein | - |
| HFQ25_RS00790 (HFQ25_00800) | 32400..32935 | + | 536 | Protein_40 | thermonuclease family protein | - |
Host bacterium
| ID | 5853 | GenBank | NZ_CP051424 |
| Plasmid name | p20723-3 | Incompatibility group | IncI2 |
| Plasmid size | 57420 bp | Coordinate of oriT [Strand] | 44166..44218 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Uganda strain CVM 20723 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |