Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105401
Name   oriT_p20746-1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Anatum strain CVM 20746
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP051453 (10540..10628 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p20746-1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3684 GenBank   WP_001474686
Name   nikB_HFQ45_RS23145_p20746-1 insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   1665 GenBank   WP_001283947
Name   WP_001283947_p20746-1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   3664 GenBank   WP_001289271
Name   trbC_HFQ45_RS23150_p20746-1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86927.12 Da        Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16006..56932

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFQ45_RS23150 (HFQ45_23155) 13722..16013 - 2292 WP_001289271 F-type conjugative transfer protein TrbC -
HFQ45_RS23155 (HFQ45_23160) 16006..17076 - 1071 WP_000151575 IncI1-type conjugal transfer protein TrbB trbB
HFQ45_RS23160 (HFQ45_23165) 17095..18303 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HFQ45_RS23165 (HFQ45_23170) 18595..18747 + 153 WP_001303307 Hok/Gef family protein -
HFQ45_RS23170 (HFQ45_23175) 18819..19070 - 252 WP_001291965 hypothetical protein -
HFQ45_RS23175 (HFQ45_23180) 19431..20213 - 783 WP_001300609 IS21-like element IS100kyp family helper ATPase IstB -
HFQ45_RS23180 (HFQ45_23185) 20210..21232 - 1023 WP_001572805 IS21-like element IS100 family transposase -
HFQ45_RS23185 (HFQ45_23190) 21953..22129 - 177 WP_001054900 hypothetical protein -
HFQ45_RS23190 (HFQ45_23195) 22521..22730 + 210 WP_000062603 HEAT repeat domain-containing protein -
HFQ45_RS23195 (HFQ45_23200) 22802..23464 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
HFQ45_RS23200 (HFQ45_23205) 23535..25703 - 2169 WP_000698368 DotA/TraY family protein traY
HFQ45_RS23205 (HFQ45_23210) 25800..26384 - 585 WP_001037998 conjugal transfer protein TraX -
HFQ45_RS23210 (HFQ45_23215) 26413..27615 - 1203 WP_001189158 IncI1-type conjugal transfer protein TraW traW
HFQ45_RS23215 (HFQ45_23220) 27582..28196 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
HFQ45_RS23220 (HFQ45_23225) 28196..31240 - 3045 WP_052869827 IncI1-type conjugal transfer protein TraU traU
HFQ45_RS23225 (HFQ45_23230) 31330..32130 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HFQ45_RS23230 (HFQ45_23235) 32114..32302 - 189 WP_001277255 putative conjugal transfer protein TraS -
HFQ45_RS23235 (HFQ45_23240) 32366..32770 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
HFQ45_RS23240 (HFQ45_23245) 32821..33348 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HFQ45_RS23245 (HFQ45_23250) 33348..34052 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HFQ45_RS23250 (HFQ45_23255) 34052..35341 - 1290 WP_001271997 conjugal transfer protein TraO traO
HFQ45_RS23255 (HFQ45_23260) 35344..36327 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
HFQ45_RS23260 (HFQ45_23265) 36338..37030 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HFQ45_RS23265 (HFQ45_23270) 37027..37374 - 348 WP_001055900 conjugal transfer protein traL
HFQ45_RS23270 (HFQ45_23275) 37392..41159 - 3768 WP_001141535 LPD7 domain-containing protein -
HFQ45_RS23275 (HFQ45_23280) 41249..41800 - 552 WP_000014584 phospholipase D family protein -
HFQ45_RS23280 (HFQ45_23285) 41815..42105 - 291 WP_001299214 hypothetical protein traK
HFQ45_RS23285 (HFQ45_23290) 42102..43250 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HFQ45_RS23290 (HFQ45_23295) 43247..44065 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
HFQ45_RS23295 (HFQ45_23300) 44062..44520 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HFQ45_RS23300 (HFQ45_23305) 44915..45499 - 585 WP_000977517 histidine phosphatase family protein -
HFQ45_RS23305 (HFQ45_23310) 45559..46761 - 1203 WP_000976353 conjugal transfer protein TraF -
HFQ45_RS23310 (HFQ45_23315) 46847..47671 - 825 WP_001238927 conjugal transfer protein TraE traE
HFQ45_RS23315 (HFQ45_23320) 47822..48976 - 1155 WP_001139958 site-specific integrase -
HFQ45_RS23320 (HFQ45_23325) 49667..49915 + 249 WP_001349157 hypothetical protein -
HFQ45_RS23325 (HFQ45_23330) 49912..50196 - 285 Protein_50 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HFQ45_RS24055 50784..51005 + 222 Protein_51 prepilin -
HFQ45_RS23335 (HFQ45_23335) 51002..52426 - 1425 WP_023278060 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HFQ45_RS23340 (HFQ45_23340) 52426..53082 - 657 WP_001193553 prepilin peptidase -
HFQ45_RS23345 (HFQ45_23345) 53067..53627 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HFQ45_RS23350 (HFQ45_23350) 53637..54251 - 615 WP_000959785 type 4 pilus major pilin -
HFQ45_RS23355 (HFQ45_23355) 54269..55366 - 1098 WP_001208805 type II secretion system F family protein -
HFQ45_RS23360 (HFQ45_23360) 55379..56932 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HFQ45_RS23365 (HFQ45_23365) 56943..57395 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
HFQ45_RS23370 (HFQ45_23370) 57382..58677 - 1296 WP_000752774 type 4b pilus protein PilO2 -
HFQ45_RS23375 (HFQ45_23375) 58670..60352 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HFQ45_RS23380 (HFQ45_23380) 60366..60803 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HFQ45_RS23385 (HFQ45_23385) 60803..61870 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   5839 GenBank   NZ_CP051453
Plasmid name   p20746-1 Incompatibility group   IncI1
Plasmid size   99888 bp Coordinate of oriT [Strand]   10540..10628 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Anatum strain CVM 20746

Cargo genes

Drug resistance gene   blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -