Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105400
• Name: oriT_p21794
• Organism: Salmonella enterica subsp. enterica serovar Agona strain CVM 21974
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP051403 (29558..29708 [+], 151 nt)
• oriT length: 151 nt
• IRs (inverted repeats): 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 151 nt

>oriT_p21794
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_HFQ69_RS23840_p21794

• ID: 3683
• GenBank: WP_001326173
• Name: mobH_HFQ69_RS23840_p21794
• UniProt ID: F5BPR1
• Length: 990 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 990 a.a.
• Molecular weight: 109356.90 Da
• Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (51-357)

Protein structure

• AlphaFold DB: F5BPR1

T4CP

traD_HFQ69_RS23845_p21794

• ID: 3663
• GenBank: WP_000178857
• Name: traD_HFQ69_RS23845_p21794
• UniProt ID: _
• Length: 621 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 621 a.a.
• Molecular weight: 69225.71 Da
• Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (170-299)

• TraM recognition site of TraD and TraG (TraG-D_C) (471-598)

Protein structure

No available structure.

Host bacterium

• ID: 5838
• GenBank: NZ_CP051403
• Plasmid name: p21794
• Incompatibility group: IncA/C2
• Plasmid size: 102040 bp
• Coordinate of oriT [Strand]: 29558..29708 [+]
• Host baterium: Salmonella enterica subsp. enterica serovar Agona strain CVM 21974

Cargo genes

• Drug resistance gene: floR, tet(A), aph(6)-Id, aph(3'')-Ib, sul2, aph(3')-Ia, blaTEM-1B, qacE, cmlA1, ant(2'')-Ia
• Virulence gene: -
• Metal resistance gene: merE, merD, merB, merA, merP, merT, merR
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -