Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105375
Name   oriT1_p30177-2 in_silico
Organism   Salmonella enterica subsp. enterica serovar Kentucky strain CVM 30177
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP051348 (21973..22053 [-], 81 nt)
oriT length   81 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 81 nt

>oriT1_p30177-2
GGGGTGCCGGGGCAAAGCCCTGACCAGACAGCAAAAGGAATATCGTCTCGGGTGACGGTATTACATTTGAACATTCTGTCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3669 GenBank   WP_012512785
Name   nikB_HFT18_RS23770_p30177-2 insolico UniProt ID   A0A5U5KNS6
Length   902 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 902 a.a.        Molecular weight: 104329.76 Da        Isoelectric Point: 7.5318

>WP_012512785.1 IncI1-type relaxase NikB [Salmonella enterica]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHPGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPAERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPVLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERHYRDYIAANSNDDASLPPPEQDKRREQPSPVLSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A5U5KNS6


Auxiliary protein

ID   1658 GenBank   WP_001284001
Name   WP_001284001_p30177-2 insolico UniProt ID   A0A5T9R3M9
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12599.50 Da        Isoelectric Point: 10.8113

>WP_001284001.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSGVRKKSEVRQKTVVRTLRFSPAEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDREYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5T9R3M9


Host bacterium

ID   5813 GenBank   NZ_CP051348
Plasmid name   p30177-2 Incompatibility group   IncX1
Plasmid size   48581 bp Coordinate of oriT [Strand]   21973..22053 [-]; 47849..47936 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Kentucky strain CVM 30177

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -