Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105372 |
| Name | oriT_p35161-2  |
| Organism | Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 35161 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP051312 (101023..101111 [-], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_p35161-2
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Auxiliary protein
| ID | 1655 | GenBank | WP_001283947 |
| Name | WP_001283947_p35161-2 |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 3640 | GenBank | WP_001289271 |
| Name | trbC_HFU19_RS24790_p35161-2 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86927.12 Da Isoelectric Point: 6.7713
>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5021..44725
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HFU19_RS24790 (HFU19_24805) | 2737..5028 | - | 2292 | WP_001289271 | F-type conjugative transfer protein TrbC | - |
| HFU19_RS24795 (HFU19_24810) | 5021..6091 | - | 1071 | WP_000151575 | IncI1-type conjugal transfer protein TrbB | trbB |
| HFU19_RS24800 (HFU19_24815) | 6110..7318 | - | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| HFU19_RS24805 (HFU19_24820) | 7610..7762 | + | 153 | WP_001303307 | Hok/Gef family protein | - |
| HFU19_RS24810 (HFU19_24825) | 7834..8085 | - | 252 | WP_001291965 | hypothetical protein | - |
| HFU19_RS24815 (HFU19_24830) | 8446..9228 | - | 783 | WP_001300609 | IS21-like element IS100kyp family helper ATPase IstB | - |
| HFU19_RS24820 (HFU19_24835) | 9225..10247 | - | 1023 | WP_001572805 | IS21-like element IS100 family transposase | - |
| HFU19_RS24825 (HFU19_24840) | 10968..11144 | - | 177 | WP_001054900 | hypothetical protein | - |
| HFU19_RS24830 (HFU19_24845) | 11536..11745 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| HFU19_RS24835 (HFU19_24850) | 11817..12479 | - | 663 | WP_000644794 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HFU19_RS24840 (HFU19_24855) | 12550..14718 | - | 2169 | WP_000698368 | DotA/TraY family protein | traY |
| HFU19_RS24845 (HFU19_24860) | 14815..15399 | - | 585 | WP_001037998 | conjugal transfer protein TraX | - |
| HFU19_RS24850 (HFU19_24865) | 15428..16630 | - | 1203 | WP_001189158 | IncI1-type conjugal transfer protein TraW | traW |
| HFU19_RS24855 (HFU19_24870) | 16597..17211 | - | 615 | WP_000337394 | IncI1-type conjugal transfer protein TraV | traV |
| HFU19_RS24860 (HFU19_24875) | 17211..20255 | - | 3045 | WP_001024751 | IncI1-type conjugal transfer protein TraU | traU |
| HFU19_RS24865 (HFU19_24880) | 20345..21145 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| HFU19_RS24870 (HFU19_24885) | 21129..21317 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| HFU19_RS24875 (HFU19_24890) | 21381..21785 | - | 405 | WP_000086960 | IncI1-type conjugal transfer protein TraR | traR |
| HFU19_RS24880 (HFU19_24895) | 21836..22363 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| HFU19_RS24885 (HFU19_24900) | 22363..23067 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| HFU19_RS24890 (HFU19_24905) | 23067..24356 | - | 1290 | WP_001271997 | conjugal transfer protein TraO | traO |
| HFU19_RS24895 (HFU19_24910) | 24359..25342 | - | 984 | WP_001191878 | IncI1-type conjugal transfer protein TraN | traN |
| HFU19_RS24900 (HFU19_24915) | 25353..26045 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| HFU19_RS24905 (HFU19_24920) | 26042..26389 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| HFU19_RS24910 (HFU19_24925) | 26407..30174 | - | 3768 | WP_001141535 | LPD7 domain-containing protein | - |
| HFU19_RS24915 (HFU19_24930) | 30264..30815 | - | 552 | WP_000014584 | phospholipase D family protein | - |
| HFU19_RS24920 (HFU19_24935) | 30830..31120 | - | 291 | WP_001299214 | hypothetical protein | traK |
| HFU19_RS24925 (HFU19_24940) | 31117..32265 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| HFU19_RS24930 (HFU19_24945) | 32262..33080 | - | 819 | WP_000646097 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| HFU19_RS24935 (HFU19_24950) | 33077..33535 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| HFU19_RS24940 (HFU19_24955) | 33930..34514 | - | 585 | WP_000977517 | histidine phosphatase family protein | - |
| HFU19_RS24945 (HFU19_24960) | 34574..35776 | - | 1203 | WP_000976353 | conjugal transfer protein TraF | - |
| HFU19_RS24950 (HFU19_24965) | 35862..36686 | - | 825 | WP_001238927 | conjugal transfer protein TraE | traE |
| HFU19_RS24955 (HFU19_24970) | 36837..37991 | - | 1155 | WP_001139958 | site-specific integrase | - |
| HFU19_RS24960 (HFU19_24975) | 38682..38930 | + | 249 | WP_001349157 | hypothetical protein | - |
| HFU19_RS24965 (HFU19_24980) | 38927..40219 | - | 1293 | WP_032274560 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFU19_RS24970 (HFU19_24985) | 40219..40875 | - | 657 | WP_001193553 | prepilin peptidase | - |
| HFU19_RS24975 (HFU19_24990) | 40860..41420 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| HFU19_RS24980 (HFU19_24995) | 41430..42044 | - | 615 | WP_000959785 | type 4 pilus major pilin | - |
| HFU19_RS24985 (HFU19_25000) | 42062..43159 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| HFU19_RS24990 (HFU19_25005) | 43172..44725 | - | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HFU19_RS24995 (HFU19_25010) | 44736..45188 | - | 453 | WP_001247336 | type IV pilus biogenesis protein PilP | - |
| HFU19_RS25000 (HFU19_25015) | 45175..46470 | - | 1296 | WP_000752774 | type 4b pilus protein PilO2 | - |
| HFU19_RS25005 (HFU19_25020) | 46463..48145 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| HFU19_RS25010 (HFU19_25025) | 48159..48596 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| HFU19_RS25015 (HFU19_25030) | 48596..49663 | - | 1068 | WP_000742600 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 5810 | GenBank | NZ_CP051312 |
| Plasmid name | p35161-2 | Incompatibility group | IncI1 |
| Plasmid size | 101468 bp | Coordinate of oriT [Strand] | 101023..101111 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 35161 |
Cargo genes
| Drug resistance gene | blaCMY-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |