Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105362
• Name: oriT_PR01996-12|unnamed_2
• Organism: Enterococcus faecium strain PR01996-12
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP064426 (9601..9792 [-], 192 nt)
• oriT length: 192 nt
• IRs (inverted repeats): 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 192 nt

>oriT_PR01996-12|unnamed_2
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_IUH71_RS14630_PR01996-12|unnamed_2

• ID: 3660
• GenBank: WP_002326870
• Name: mobP2_IUH71_RS14630_PR01996-12|unnamed_2
• UniProt ID: B5U8V6
• Length: 506 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 506 a.a.
• Molecular weight: 60597.01 Da
• Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (11-438)

Protein structure

• AlphaFold DB: B5U8V6

T4CP

t4cp2_IUH71_RS14915_PR01996-12|unnamed_2

• ID: 3632
• GenBank: WP_002311855
• Name: t4cp2_IUH71_RS14915_PR01996-12|unnamed_2
• UniProt ID: _
• Length: 952 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 952 a.a.
• Molecular weight: 108225.12 Da
• Isoelectric Point: 7.4751

>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (199-442)

• TraM recognition site of TraD and TraG (TraG-D_C) (589-701)

Protein structure

No available structure.

Host bacterium

• ID: 5800
• GenBank: NZ_CP064426
• Plasmid name: PR01996-12|unnamed_2
• Incompatibility group: -
• Plasmid size: 65409 bp
• Coordinate of oriT [Strand]: 9601..9792 [-]
• Host baterium: Enterococcus faecium strain PR01996-12

Cargo genes

• Drug resistance gene: dfrG
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA1