Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105361 |
| Name | oriT_PR05720-3|unnamed_3  |
| Organism | Enterococcus faecium strain PR05720-3 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP064409 (17371..17408 [+], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_PR05720-3|unnamed_3
ATATGAAGTAACGAAGTTAGTGCGTATAAGTGCGCCCT
ATATGAAGTAACGAAGTTAGTGCGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3659 | GenBank | WP_244829795 |
| Name | MobA_MobL_IUH68_RS14270_PR05720-3|unnamed_3 |
UniProt ID | _ |
| Length | 664 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 664 a.a. Molecular weight: 77719.63 Da Isoelectric Point: 9.0094
>WP_244829795.1 MobA/MobL family protein [Enterococcus faecium]
MIQLAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFISKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCRELNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQENVLKQ
DKNFTLKFEKTFSPLEKKELKNLSQELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEE
SKKIFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
YIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELEKNEPEKNEVSVKMFQFAKSINRL
LSGNQPQKKRNLDKLIKQTKAKENQSLQRNIPLR
MIQLAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFISKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCRELNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQENVLKQ
DKNFTLKFEKTFSPLEKKELKNLSQELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEE
SKKIFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
YIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELEKNEPEKNEVSVKMFQFAKSINRL
LSGNQPQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3631 | GenBank | WP_010706428 |
| Name | t4cp2_IUH68_RS14225_PR05720-3|unnamed_3 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63130.14 Da Isoelectric Point: 4.7040
>WP_010706428.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2387..14913
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IUH68_RS14180 (IUH68_14175) | 294..932 | - | 639 | WP_212463073 | sugar O-acetyltransferase | - |
| IUH68_RS14185 (IUH68_14180) | 959..1342 | - | 384 | WP_212463097 | transcriptional regulator, BlaI/MecI/CopY family protein | - |
| IUH68_RS14190 (IUH68_14185) | 1684..2010 | - | 327 | WP_010706320 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| IUH68_RS14195 (IUH68_14190) | 2011..2244 | - | 234 | WP_010706321 | hypothetical protein | - |
| IUH68_RS14200 (IUH68_14195) | 2387..3175 | - | 789 | WP_212463074 | LPXTG cell wall anchor domain-containing protein | prgC |
| IUH68_RS14205 (IUH68_14200) | 3527..3880 | - | 354 | WP_212463075 | hypothetical protein | - |
| IUH68_RS14210 (IUH68_14205) | 3909..4877 | - | 969 | WP_115234509 | hypothetical protein | - |
| IUH68_RS14215 (IUH68_14210) | 4894..5826 | - | 933 | WP_212463076 | conjugal transfer protein TrbL family protein | trsL |
| IUH68_RS14220 (IUH68_14215) | 5826..6749 | - | 924 | WP_212463077 | hypothetical protein | - |
| IUH68_RS14225 (IUH68_14220) | 6767..8422 | - | 1656 | WP_010706428 | VirD4-like conjugal transfer protein, CD1115 family | - |
| IUH68_RS14230 (IUH68_14225) | 8415..8846 | - | 432 | WP_163327513 | hypothetical protein | - |
| IUH68_RS14235 (IUH68_14230) | 8851..9402 | - | 552 | WP_141062720 | hypothetical protein | - |
| IUH68_RS14240 (IUH68_14235) | 9415..10524 | - | 1110 | WP_212463078 | lysozyme family protein | orf14 |
| IUH68_RS14245 (IUH68_14240) | 10546..11898 | - | 1353 | WP_212463079 | conjugal transfer protein TraF | trsF |
| IUH68_RS14250 (IUH68_14245) | 11912..13873 | - | 1962 | WP_212463080 | TrsE protein | virb4 |
| IUH68_RS14255 (IUH68_14250) | 13884..14513 | - | 630 | WP_212463081 | hypothetical protein | - |
| IUH68_RS14260 (IUH68_14255) | 14530..14913 | - | 384 | WP_001083514 | glycosyl transferase | trsC |
| IUH68_RS14265 (IUH68_14260) | 14932..15264 | - | 333 | WP_212463082 | CagC family type IV secretion system protein | - |
| IUH68_RS14270 (IUH68_14265) | 15288..17273 | - | 1986 | WP_212463083 | MobQ family relaxase | - |
| IUH68_RS14275 (IUH68_14270) | 17565..17864 | + | 300 | WP_010782377 | hypothetical protein | - |
| IUH68_RS14280 (IUH68_14275) | 17867..18124 | + | 258 | WP_212463084 | hypothetical protein | - |
| IUH68_RS14285 (IUH68_14280) | 18242..19486 | + | 1245 | WP_212463085 | IS256 family transposase | - |
| IUH68_RS14290 (IUH68_14285) | 19629..19862 | - | 234 | WP_244829797 | thymidylate synthase ThyX | - |
Host bacterium
| ID | 5799 | GenBank | NZ_CP064409 |
| Plasmid name | PR05720-3|unnamed_3 | Incompatibility group | - |
| Plasmid size | 44860 bp | Coordinate of oriT [Strand] | 17371..17408 [+] |
| Host baterium | Enterococcus faecium strain PR05720-3 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA14 |