Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105335
Name   oriT_p1423_1 in_silico
Organism   Klebsiella pneumoniae strain C0674
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP061833 (153631..153680 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_p1423_1
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3641 GenBank   WP_015065542
Name   traI_IFX77_RS26790_p1423_1 insolico UniProt ID   K7R3U7
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190318.03 Da        Isoelectric Point: 6.2890

>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4CP

ID   3608 GenBank   WP_015065541
Name   traD_IFX77_RS26795_p1423_1 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86025.00 Da        Isoelectric Point: 5.0577

>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 124211..154238

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFX77_RS26795 (IFX77_26800) 124211..126523 - 2313 WP_015065541 type IV conjugative transfer system coupling protein TraD virb4
IFX77_RS26800 (IFX77_26805) 126652..127341 - 690 WP_072198073 hypothetical protein -
IFX77_RS26805 (IFX77_26810) 127534..128265 - 732 WP_016831056 conjugal transfer complement resistance protein TraT -
IFX77_RS26810 (IFX77_26815) 128516..129118 - 603 WP_071604857 hypothetical protein -
IFX77_RS26815 (IFX77_26820) 129141..131963 - 2823 WP_016831055 conjugal transfer mating-pair stabilization protein TraG traG
IFX77_RS26820 (IFX77_26825) 131963..133342 - 1380 WP_072159542 conjugal transfer pilus assembly protein TraH traH
IFX77_RS26825 (IFX77_26830) 133320..133763 - 444 WP_016831053 F-type conjugal transfer protein TrbF -
IFX77_RS26830 (IFX77_26835) 133809..134366 - 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
IFX77_RS26835 (IFX77_26840) 134338..134577 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
IFX77_RS26840 (IFX77_26845) 134588..135340 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
IFX77_RS26845 (IFX77_26850) 135361..135687 - 327 WP_016831051 hypothetical protein -
IFX77_RS26850 (IFX77_26855) 135700..135948 - 249 WP_004152675 hypothetical protein -
IFX77_RS26855 (IFX77_26860) 135926..136180 - 255 WP_015065558 conjugal transfer protein TrbE -
IFX77_RS26860 (IFX77_26865) 136212..138167 - 1956 WP_023313940 type-F conjugative transfer system mating-pair stabilization protein TraN traN
IFX77_RS26865 (IFX77_26870) 138226..138873 - 648 WP_039109927 type-F conjugative transfer system pilin assembly protein TrbC trbC
IFX77_RS26870 (IFX77_26875) 138952..139128 - 177 WP_162866177 hypothetical protein -
IFX77_RS26875 (IFX77_26880) 139149..140138 - 990 WP_015632509 conjugal transfer pilus assembly protein TraU traU
IFX77_RS26880 (IFX77_26885) 140135..140536 - 402 WP_016831047 hypothetical protein -
IFX77_RS26885 (IFX77_26890) 140571..141206 - 636 WP_016831046 type-F conjugative transfer system protein TraW traW
IFX77_RS26890 (IFX77_26895) 141206..141595 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
IFX77_RS26895 (IFX77_26900) 141595..144234 - 2640 WP_016831045 type IV secretion system protein TraC virb4
IFX77_RS26900 (IFX77_26905) 144306..144704 - 399 WP_023158008 hypothetical protein -
IFX77_RS27695 144712..144930 - 219 WP_072196435 hypothetical protein -
IFX77_RS27700 144944..145102 - 159 WP_223178560 hypothetical protein -
IFX77_RS26910 (IFX77_26915) 145145..145549 - 405 WP_016831042 hypothetical protein -
IFX77_RS26915 (IFX77_26920) 145616..145927 - 312 WP_016831041 hypothetical protein -
IFX77_RS26920 (IFX77_26925) 145928..146146 - 219 WP_014386199 hypothetical protein -
IFX77_RS26925 (IFX77_26930) 146170..146459 - 290 Protein_165 hypothetical protein -
IFX77_RS26930 (IFX77_26935) 146464..146874 - 411 WP_032409654 hypothetical protein -
IFX77_RS26935 (IFX77_26940) 147006..147590 - 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
IFX77_RS26940 (IFX77_26945) 147613..148209 - 597 Protein_168 conjugal transfer pilus-stabilizing protein TraP -
IFX77_RS26945 (IFX77_26950) 148202..149626 - 1425 WP_016831036 F-type conjugal transfer pilus assembly protein TraB traB
IFX77_RS26950 (IFX77_26955) 149626..150366 - 741 WP_032408905 type-F conjugative transfer system secretin TraK traK
IFX77_RS26955 (IFX77_26960) 150353..150919 - 567 WP_016831035 type IV conjugative transfer system protein TraE traE
IFX77_RS26960 (IFX77_26965) 150939..151244 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
IFX77_RS26965 (IFX77_26970) 151258..151626 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
IFX77_RS26970 (IFX77_26975) 151688..151894 - 207 WP_171773970 TraY domain-containing protein -
IFX77_RS26975 (IFX77_26980) 152027..152746 - 720 WP_016831034 conjugal transfer protein TrbJ -
IFX77_RS26980 (IFX77_26985) 152946..153362 - 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
IFX77_RS26985 (IFX77_26990) 153753..154238 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
IFX77_RS26990 (IFX77_26995) 154271..154600 - 330 WP_011977736 DUF5983 family protein -
IFX77_RS26995 (IFX77_27000) 154633..155454 - 822 WP_004182076 DUF932 domain-containing protein -
IFX77_RS27000 (IFX77_27005) 156287..156700 - 414 WP_004182074 helix-turn-helix domain-containing protein -
IFX77_RS27005 (IFX77_27010) 156701..156979 - 279 WP_004152721 helix-turn-helix transcriptional regulator -
IFX77_RS27010 (IFX77_27015) 156969..157289 - 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
IFX77_RS27015 (IFX77_27020) 157370..157594 - 225 WP_004152719 hypothetical protein -
IFX77_RS27020 (IFX77_27025) 157605..157817 - 213 WP_004182070 hypothetical protein -
IFX77_RS27025 (IFX77_27030) 157878..158234 - 357 WP_004152717 hypothetical protein -
IFX77_RS27030 (IFX77_27035) 158870..159220 - 351 WP_004182068 hypothetical protein -

Host bacterium

ID   5773 GenBank   NZ_CP061833
Plasmid name   p1423_1 Incompatibility group   IncFIB
Plasmid size   193300 bp Coordinate of oriT [Strand]   153631..153680 [+]
Host baterium   Klebsiella pneumoniae strain C0674

Cargo genes

Drug resistance gene   qnrB19, aac(3)-IIa, blaOXA-1, aac(6')-Ib-cr, tet(A), qnrB1, blaCTX-M-15, dfrA14
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9