Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105314
Name   oriT_pRHBSTW-00832_5 in_silico
Organism   Klebsiella pneumoniae strain RHBSTW-00832
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP056311 (22671..24376 [-], 1706 nt)
oriT length   1706 nt
IRs (inverted repeats)      655..664, 672..681  (TTTTTTAAAA..TTTTAAAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1706 nt

>oriT_pRHBSTW-00832_5
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAAATTAAAGTCATTATGTTGGCAGCAATAGTTACAGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCTGAGCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCCACGATACAATATGGACTCCAGCAGTATTTTACGCAGCCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCTAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGTGTTATTGAATACCAGCCCGTCCTTAATTGTCTGACATGCAGAGAAACCACTATTTATTTCTCTCGCCAATCCATTTAATCAACACAATCTAATAAGGATTTACCATGAGAAAAACCGTTATCACGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCATTTGCCGCTGACCCATGCGAAGTTGTTCTTTGCATGTACGGAAAAGCAACGGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCTTTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGCGAATGTAAAGCCGCAGACCCTGCATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTATGAAATATACGAAAGCTGTTTTATAGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCCGACGCAAATAAATTAATGAGGTACGCAGAAAGTAAAGGCACGAAAATTGATGATGTGTCCTACAGTGAAGCACTGGAAGCCGAAAGGGAGTGCAGAGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGTATTGTACAATACGCAGACTAACAGGCGGCGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTGCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGAAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCCTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   3600 GenBank   WP_009309962
Name   t4cp2_HV292_RS29500_pRHBSTW-00832_5 insolico UniProt ID   _
Length   537 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 537 a.a.        Molecular weight: 61059.67 Da        Isoelectric Point: 6.6643

>WP_009309962.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Klebsiella]
MDSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNADFVQS
LREDVPAFCGELEEIALVNSINAKNGFSLKEVFDDGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDR
INSTPRTVAIFLDELKYHLSRPALEGLGTARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFK
LIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVF
TSTELPRATKMFQVQVKKENIELLSFEYKQATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(185-281)

TraM recognition site of TraD and TraG (TraG-D_C)

(358-449)

  Protein structure



No available structure.



ID   3601 GenBank   WP_009309970
Name   t4cp2_HV292_RS29555_pRHBSTW-00832_5 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103094.06 Da        Isoelectric Point: 7.4625

>WP_009309970.1 MULTISPECIES: VirB3 family type IV secretion system protein [Klebsiella]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGEPTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(534-813)

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20841..34256

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV292_RS29465 (HV292_29465) 16581..17165 - 585 WP_009309957 recombinase family protein -
HV292_RS29470 (HV292_29470) 17324..18085 + 762 WP_032451463 SprT-like domain-containing protein -
HV292_RS29475 (HV292_29475) 18206..18853 + 648 WP_004178130 ParA family protein -
HV292_RS29480 (HV292_29480) 18894..19127 + 234 WP_004178129 hypothetical protein -
HV292_RS29485 (HV292_29485) 19165..19461 - 297 WP_227670696 hypothetical protein -
HV292_RS29490 (HV292_29490) 19567..20040 - 474 WP_009309960 thermonuclease family protein -
HV292_RS29495 (HV292_29495) 20077..20832 - 756 WP_009309961 MobC family replication-relaxation protein -
HV292_RS29500 (HV292_29500) 20841..22454 - 1614 WP_009309962 type IV secretion system DNA-binding domain-containing protein virb4
HV292_RS29505 (HV292_29505) 23234..23539 - 306 WP_009309963 hypothetical protein -
HV292_RS29510 (HV292_29510) 23573..23878 - 306 WP_071986602 conjugal transfer protein -
HV292_RS29515 (HV292_29515) 23905..24312 - 408 WP_071986603 cag pathogenicity island Cag12 family protein -
HV292_RS29520 (HV292_29520) 24309..25334 - 1026 WP_009309964 P-type DNA transfer ATPase VirB11 virB11
HV292_RS29525 (HV292_29525) 25324..26592 - 1269 WP_009309965 VirB10/TraB/TrbI family type IV secretion system protein virB10
HV292_RS29530 (HV292_29530) 26636..27544 - 909 WP_074195926 P-type conjugative transfer protein VirB9 virB9
HV292_RS29535 (HV292_29535) 27541..28224 - 684 WP_009309967 type IV secretion system protein virB8
HV292_RS29540 (HV292_29540) 28446..29522 - 1077 WP_009309968 type IV secretion system protein virB6
HV292_RS29545 (HV292_29545) 29534..29761 - 228 WP_004178109 EexN family lipoprotein -
HV292_RS29550 (HV292_29550) 29771..30475 - 705 WP_009309969 type IV secretion system protein -
HV292_RS29555 (HV292_29555) 30493..33231 - 2739 WP_009309970 VirB3 family type IV secretion system protein virb4
HV292_RS29560 (HV292_29560) 33244..33549 - 306 WP_009309971 TrbC/VirB2 family protein virB2
HV292_RS29565 (HV292_29565) 33546..34256 - 711 WP_009309972 lytic transglycosylase domain-containing protein virB1
HV292_RS29570 (HV292_29570) 34374..34592 - 219 WP_004178172 hypothetical protein -
HV292_RS29575 (HV292_29575) 34597..34812 - 216 WP_004178170 hypothetical protein -
HV292_RS29580 (HV292_29580) 34884..35339 - 456 WP_009309974 transcription termination/antitermination NusG family protein -
HV292_RS29585 (HV292_29585) 35629..35967 + 339 WP_306460784 helix-turn-helix transcriptional regulator -

Host bacterium

ID   5752 GenBank   NZ_CP056311
Plasmid name   pRHBSTW-00832_5 Incompatibility group   IncFII
Plasmid size   36438 bp Coordinate of oriT [Strand]   22671..24376 [-]
Host baterium   Klebsiella pneumoniae strain RHBSTW-00832

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -