Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105287
• Name: oriT_pRHBSTW-00355_4
• Organism: Citrobacter freundii strain RHBSTW-00355
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP056589 (12123..12212 [-], 90 nt)
• oriT length: 90 nt
• IRs (inverted repeats): 34..41, 44..51 (AGTGCGAT..ATCGCACT) 20..25, 39..44 (TAAATC..GATTTA)
• Location of nic site: 62..63
• Conserved sequence flanking the nic site: GGTGTATAGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 90 nt

>oriT_pRHBSTW-00355_4
TATTTCATTTTTTGTTTTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobF_HV173_RS26870_pRHBSTW-00355_4

• ID: 3618
• GenBank: WP_050136475
• Name: mobF_HV173_RS26870_pRHBSTW-00355_4
• UniProt ID: _
• Length: 1072 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1072 a.a.
• Molecular weight: 118873.51 Da
• Isoelectric Point: 6.4635

>WP_050136475.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGDIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDVEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNDSQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGYNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNSKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

Protein domains

Predicted by InterproScan.

• TrwC relaxase (TrwC) (16-288)

• AAA domain (AAA_30) (480-656)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (855-899)

Protein structure

No available structure.

T4CP

t4cp2_HV173_RS26865_pRHBSTW-00355_4

• ID: 3584
• GenBank: WP_040223430
• Name: t4cp2_HV173_RS26865_pRHBSTW-00355_4
• UniProt ID: _
• Length: 510 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 510 a.a.
• Molecular weight: 57917.09 Da
• Isoelectric Point: 9.6871

>WP_040223430.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFTYGINSETAHYLVPYLLKNTFNLWPLWAAIIAGVVIGLCGFI
GFIIYDKGRVYKGPRFKRILRGTKLARASTLAEKTKERGKKQLMIAGVPVPTEYETLHFSIGGATGTGKS
TVIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRNPTMEEVMHWCTIVENDKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKYVKYTRKNPVPGIIMR

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (113-492)

Protein structure

No available structure.

Host bacterium

• ID: 5725
• GenBank: NZ_CP056589
• Plasmid name: pRHBSTW-00355_4
• Incompatibility group: IncN3
• Plasmid size: 63589 bp
• Coordinate of oriT [Strand]: 12123..12212 [-]
• Host baterium: Citrobacter freundii strain RHBSTW-00355

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -