Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 105275
• Name: oriT_pRHB23-C01_5
• Organism: Escherichia fergusonii strain RHB23-C01
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP057569 (16909..17011 [-], 103 nt)
• oriT length: 103 nt
• IRs (inverted repeats): 65..70, 73..78 (CACCGT..ACGGTG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 103 nt

>oriT_pRHB23-C01_5
AGCCTCGCAGAGCAGGACTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTAGAAGCACCGTTTACGGTGGGCCTACTTTACATGTCCTGCCCGG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HVX91_RS23410_pRHB23-C01_5

• ID: 3611
• GenBank: WP_023352245
• Name: Relaxase_HVX91_RS23410_pRHB23-C01_5
• UniProt ID: _
• Length: 877 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 877 a.a.
• Molecular weight: 97961.42 Da
• Isoelectric Point: 10.5942

>WP_023352245.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MISRHIPMRNVKKSSFFRLAEYLQDSQNKQERVGAVKTTNCHQENILDAARYEIEPTQRLNTRAKGDKTY
HLVISFRPGENPPQEVLDVIEERMCTALGYGEHQRVSAVHYDTDNVHIQIAINKVHPKTHNIHTPYNDYK
IRTAVCQELEKEFGLEKDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHKAMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRLSPEAIKQRFGSAVHV
KRPGERPPPIRENRQVHLGEIPVMSINNGKRYEGKPLGSQGASTSVLYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRTGRLKRAAIKLLKGPGVNKRHLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKILRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFTDPELEKLRQILAKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTEA
GRERSGNGNATGANEPGVTGRSPSADAGNGYGTSNSGRRTGSGDGVTTAGSQPGRSDDTRTGRSRAAESN
GERGHRKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLPGNVPDQLEHKTAERTDQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR

Protein domains

Predicted by InterproScan.

• Large polyvalent protein-associated domain 7 (LPD7) (471-555)

• Domain of unknown function (DUF5710) (DUF5710) (565-606)

• Relaxase/Mobilisation nuclease domain (Relaxase) (12-251)

Protein structure

No available structure.

T4CP

t4cp2_HVX91_RS23420_pRHB23-C01_5

• ID: 3574
• GenBank: WP_096217383
• Name: t4cp2_HVX91_RS23420_pRHB23-C01_5
• UniProt ID: _
• Length: 647 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 647 a.a.
• Molecular weight: 71689.89 Da
• Isoelectric Point: 8.3842

>WP_096217383.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Escherichia]
MRKPNNAVGPQVRNKKKAAKGKLIPAMAITSLVAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHERLPDAFFAAGSVGAAVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIRDAGLLNDEGVY
VGAWEDKNGQLRYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYAK
NLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVLY
KHKNEGTPATLPYVDSIMADPERGAGELWMEMTQYSHINGENHPVVGAAGRDMMDRPEEEAGSVLSTAKS
YLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERVD
NNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLGMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQDI
NQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTIQ
EVQRPLLTVDECLRMPGPKKNAEGLITERGDMVIYVAGFPAIYGKQPLFFQDEIFSLRASVPAPKVTDRI
RAVAAANDDASNEAIAI

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (445-629)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (121-417)

Protein structure

No available structure.

Host bacterium

• ID: 5713
• GenBank: NZ_CP057569
• Plasmid name: pRHB23-C01_5
• Incompatibility group: IncP1
• Plasmid size: 41486 bp
• Coordinate of oriT [Strand]: 16909..17011 [-]
• Host baterium: Escherichia fergusonii strain RHB23-C01

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -