Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105248
Name   oriT_pRHBSTW-00472_5 in_silico
Organism   Klebsiella pneumoniae strain RHBSTW-00472
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP055513 (21470..23175 [-], 1706 nt)
oriT length   1706 nt
IRs (inverted repeats)      655..664, 672..681  (TTTTTTAAAA..TTTTAAAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1706 nt

>oriT_pRHBSTW-00472_5
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAAATTAAAGTCATTATGTTGGCAGCAATAGTTACAGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCTGAGCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCCACGATACAATATGGACTCCAGCAGTATTTTACGCAGCCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCTAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGTGTTATTGAATACCAGCCCGTCCTTAATTGTCTGACATGCAGAGAAACCACTATTTATTTCTCTCGCCAATCCATTTAATCAACACAATCTAATAAGGATTTACCATGAGAAAAACCGTTATCACGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCATTTGCCGCTGACCCATGCGAAGTTGTTCTTTGCATGTACGGAAAAGCAACGGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCTTTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGCGAATGTAAAGCCGCAGACCCTGCATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTATGAAATATACGAAAGCTGTTTTATAGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCCGACGCAAATAAATTAATGAGGTACGCAGAAAGTAAAGGCACGAAAATTGATGATGTGTCCTACAGTGAAGCACTGGAAGCCGAAAGGGAGTGCAGAGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGTATTGTACAATACGCAGACTAACAGGCGGCGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTGCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGAAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCCTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   3564 GenBank   WP_009309962
Name   t4cp2_HV207_RS30025_pRHBSTW-00472_5 insolico UniProt ID   _
Length   537 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 537 a.a.        Molecular weight: 61059.67 Da        Isoelectric Point: 6.6643

>WP_009309962.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Klebsiella]
MDSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNADFVQS
LREDVPAFCGELEEIALVNSINAKNGFSLKEVFDDGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDR
INSTPRTVAIFLDELKYHLSRPALEGLGTARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFK
LIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVF
TSTELPRATKMFQVQVKKENIELLSFEYKQATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(185-281)

TraM recognition site of TraD and TraG (TraG-D_C)

(358-449)

  Protein structure



No available structure.



ID   3565 GenBank   WP_009309970
Name   t4cp2_HV207_RS30080_pRHBSTW-00472_5 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103094.06 Da        Isoelectric Point: 7.4625

>WP_009309970.1 MULTISPECIES: VirB3 family type IV secretion system protein [Klebsiella]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGEPTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(534-813)

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 19640..33055

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV207_RS29990 (HV207_30000) 15380..15964 - 585 WP_009309957 recombinase family protein -
HV207_RS29995 (HV207_30005) 16123..16884 + 762 WP_032451463 SprT-like domain-containing protein -
HV207_RS30000 (HV207_30010) 17005..17652 + 648 WP_004178130 ParA family protein -
HV207_RS30005 (HV207_30015) 17693..17926 + 234 WP_004178129 hypothetical protein -
HV207_RS30010 (HV207_30020) 17964..18260 - 297 WP_227670696 hypothetical protein -
HV207_RS30015 (HV207_30025) 18366..18839 - 474 WP_009309960 thermonuclease family protein -
HV207_RS30020 (HV207_30030) 18876..19631 - 756 WP_009309961 MobC family replication-relaxation protein -
HV207_RS30025 (HV207_30035) 19640..21253 - 1614 WP_009309962 type IV secretion system DNA-binding domain-containing protein virb4
HV207_RS30030 (HV207_30040) 22033..22338 - 306 WP_009309963 hypothetical protein -
HV207_RS30035 (HV207_30045) 22372..22677 - 306 WP_071986602 conjugal transfer protein -
HV207_RS30040 (HV207_30050) 22704..23111 - 408 WP_071986603 cag pathogenicity island Cag12 family protein -
HV207_RS30045 (HV207_30055) 23108..24133 - 1026 WP_009309964 P-type DNA transfer ATPase VirB11 virB11
HV207_RS30050 (HV207_30060) 24123..25391 - 1269 WP_009309965 VirB10/TraB/TrbI family type IV secretion system protein virB10
HV207_RS30055 (HV207_30065) 25435..26343 - 909 WP_074195926 P-type conjugative transfer protein VirB9 virB9
HV207_RS30060 (HV207_30070) 26340..27023 - 684 WP_009309967 type IV secretion system protein virB8
HV207_RS30065 (HV207_30075) 27245..28321 - 1077 WP_009309968 type IV secretion system protein virB6
HV207_RS30070 (HV207_30080) 28333..28560 - 228 WP_004178109 EexN family lipoprotein -
HV207_RS30075 (HV207_30085) 28570..29274 - 705 WP_009309969 type IV secretion system protein -
HV207_RS30080 (HV207_30090) 29292..32030 - 2739 WP_009309970 VirB3 family type IV secretion system protein virb4
HV207_RS30085 (HV207_30095) 32043..32348 - 306 WP_009309971 TrbC/VirB2 family protein virB2
HV207_RS30090 (HV207_30100) 32345..33055 - 711 WP_009309972 lytic transglycosylase domain-containing protein virB1
HV207_RS30095 (HV207_30105) 33173..33391 - 219 WP_004178172 hypothetical protein -
HV207_RS30100 (HV207_30110) 33396..33611 - 216 WP_004178170 hypothetical protein -
HV207_RS30105 (HV207_30115) 33683..34138 - 456 WP_009309974 transcription termination/antitermination NusG family protein -
HV207_RS30110 (HV207_30120) 34428..34766 + 339 WP_306460784 helix-turn-helix transcriptional regulator -

Host bacterium

ID   5686 GenBank   NZ_CP055513
Plasmid name   pRHBSTW-00472_5 Incompatibility group   IncFII
Plasmid size   35237 bp Coordinate of oriT [Strand]   21470..23175 [-]
Host baterium   Klebsiella pneumoniae strain RHBSTW-00472

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -