Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 105246 |
Name | oriT_pRHBSTW-00472_4 |
Organism | Klebsiella pneumoniae strain RHBSTW-00472 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP055512 (55455..55503 [-], 49 nt) |
oriT length | 49 nt |
IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
Location of nic site | 32..33 |
Conserved sequence flanking the nic site |
GGTGTGGTGA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pRHBSTW-00472_4
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 3603 | GenBank | WP_181247953 |
Name | traI_HV207_RS29880_pRHBSTW-00472_4 | UniProt ID | _ |
Length | 1752 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190153.89 Da Isoelectric Point: 5.9563
>WP_181247953.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLDGKVDKQVFTDLLKGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSVMAMIGGDKRLIDAHNQAVTEAVKQVETLAATRVMTDGKSETVLTGNLI
VAKFNHDTNRNQEPQLHTHAVVINATQNGDKWQSLGTDTVGKTGFIENVYASQIAFGKLYREMLKPLVEK
LGYETEVTGKHGMWEMKDVPVEPFSTRSQEVREAAGPDASLKSRDVATLDTRKSKETLDPAEKMVEWVNT
LKETGFDIHSYREDADKRAAEITRAPVSPVKTDGPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
RPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEVQRQNHVSLTPDASVVRQAPF
SDAVSVLAQDRPAMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSRDFLAGDARLAGETVTG
KSALQDGTAFIPGGTLVVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPDKHVRYSRLAQEFAVSVREGQESVAQISGTREQNALNNVIRDTLKNEGVLGAKEI
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRKHDRFVIDRVTASSNMLTLRDKEGERLDLKVSAVD
SQWTLFRADKLPVAEGERLAVLGKIPDTRLKGGESVTVLKAEAGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVENPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQDVARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQAIHLSIPLLESENLTFTRPQLLATALETGGGKVPMAEIDATIQ
SQIKAGSLLNVPVAPGQGNDLLISRQTWDAEKSILTKVLEGKDAVPPLMDSVPGSLMADLTSGQRAATRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQAPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPAQEKAIQK
ALSEGEILPAGQPATLYEALVKDYADRTPEAQLQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKAVSGDSVTLSDGKTTRTLNPKADEAQRHIDLAYAITA
HGAQGASEPYAIALEGVTGGRKQMASFESAYVGLSRMKLHVQVYTDSREGWVNAIQASPSKATAHDILAP
RNERAVKTADLLFGRAQPLNETAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKNAGIW
LSPLTDKDGRLEAIGGDGRVMGNEEARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMGRQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLSREKTLG
GD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLDGKVDKQVFTDLLKGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSVMAMIGGDKRLIDAHNQAVTEAVKQVETLAATRVMTDGKSETVLTGNLI
VAKFNHDTNRNQEPQLHTHAVVINATQNGDKWQSLGTDTVGKTGFIENVYASQIAFGKLYREMLKPLVEK
LGYETEVTGKHGMWEMKDVPVEPFSTRSQEVREAAGPDASLKSRDVATLDTRKSKETLDPAEKMVEWVNT
LKETGFDIHSYREDADKRAAEITRAPVSPVKTDGPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
RPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEVQRQNHVSLTPDASVVRQAPF
SDAVSVLAQDRPAMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSRDFLAGDARLAGETVTG
KSALQDGTAFIPGGTLVVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPDKHVRYSRLAQEFAVSVREGQESVAQISGTREQNALNNVIRDTLKNEGVLGAKEI
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRKHDRFVIDRVTASSNMLTLRDKEGERLDLKVSAVD
SQWTLFRADKLPVAEGERLAVLGKIPDTRLKGGESVTVLKAEAGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVENPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQDVARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQAIHLSIPLLESENLTFTRPQLLATALETGGGKVPMAEIDATIQ
SQIKAGSLLNVPVAPGQGNDLLISRQTWDAEKSILTKVLEGKDAVPPLMDSVPGSLMADLTSGQRAATRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQAPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPAQEKAIQK
ALSEGEILPAGQPATLYEALVKDYADRTPEAQLQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKAVSGDSVTLSDGKTTRTLNPKADEAQRHIDLAYAITA
HGAQGASEPYAIALEGVTGGRKQMASFESAYVGLSRMKLHVQVYTDSREGWVNAIQASPSKATAHDILAP
RNERAVKTADLLFGRAQPLNETAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKNAGIW
LSPLTDKDGRLEAIGGDGRVMGNEEARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMGRQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLSREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 1613 | GenBank | WP_022644939 |
Name | WP_022644939_pRHBSTW-00472_4 | UniProt ID | W8QGP1 |
Length | 130 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 130 a.a. Molecular weight: 15048.16 Da Isoelectric Point: 4.5248
>WP_022644939.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVAEKINAIVEKRRVEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LEYVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMLRFFPDEDNEVEE
MAKVQAYVSDEVAEKINAIVEKRRVEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LEYVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMLRFFPDEDNEVEE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | W8QGP1 |
T4CP
ID | 3563 | GenBank | WP_181247952 |
Name | traD_HV207_RS29875_pRHBSTW-00472_4 | UniProt ID | _ |
Length | 766 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 85822.74 Da Isoelectric Point: 4.9170
>WP_181247952.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella/Raoultella group]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTVWCGEQLWTAFVFAAIVSLTVCVVTFFVASWVLGRQGKQ
QSEDEDTGGRQLSDKPKDVARQIKRDGVASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEREYLKPSEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRMDGRLSAQLEAREAEGSHARMLFTPDEVAKDEGDTGNSPEAIKPAGDRIP
TPVNATPPEKTPPAEASGNAPVTPQPATPAMRVTTVPLTRPRPVVPASGATAVAAATDVSAATSGGTAQE
LAQQPVEADQNMLPAGMNEDGVIEDMQAYDAWLEGEQIQRDMQRREEVNINHSRSEQDDIEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTVWCGEQLWTAFVFAAIVSLTVCVVTFFVASWVLGRQGKQ
QSEDEDTGGRQLSDKPKDVARQIKRDGVASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEREYLKPSEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRMDGRLSAQLEAREAEGSHARMLFTPDEVAKDEGDTGNSPEAIKPAGDRIP
TPVNATPPEKTPPAEASGNAPVTPQPATPAMRVTTVPLTRPRPVVPASGATAVAAATDVSAATSGGTAQE
LAQQPVEADQNMLPAGMNEDGVIEDMQAYDAWLEGEQIQRDMQRREEVNINHSRSEQDDIEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 59080..84006
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HV207_RS29695 (HV207_29705) | 54323..54865 | + | 543 | WP_181247948 | antirestriction protein | - |
HV207_RS29700 (HV207_29710) | 54888..55310 | - | 423 | WP_020315406 | transglycosylase SLT domain-containing protein | - |
HV207_RS29705 (HV207_29715) | 55816..56208 | + | 393 | WP_022644939 | conjugal transfer relaxosome DNA-binding protein TraM | - |
HV207_RS29710 (HV207_29720) | 56443..56940 | + | 498 | WP_181247949 | hypothetical protein | - |
HV207_RS29715 (HV207_29725) | 56979..57959 | - | 981 | WP_009310076 | IS5-like element ISKpn26 family transposase | - |
HV207_RS29720 (HV207_29730) | 58429..58629 | + | 201 | WP_060415469 | TraY domain-containing protein | - |
HV207_RS29725 (HV207_29735) | 58698..59066 | + | 369 | WP_060415470 | type IV conjugative transfer system pilin TraA | - |
HV207_RS29730 (HV207_29740) | 59080..59385 | + | 306 | WP_053390196 | type IV conjugative transfer system protein TraL | traL |
HV207_RS29735 (HV207_29745) | 59405..59971 | + | 567 | WP_053390197 | type IV conjugative transfer system protein TraE | traE |
HV207_RS29740 (HV207_29750) | 59958..60692 | + | 735 | WP_053390198 | type-F conjugative transfer system secretin TraK | traK |
HV207_RS29745 (HV207_29755) | 60692..62113 | + | 1422 | WP_075606927 | F-type conjugal transfer pilus assembly protein TraB | traB |
HV207_RS29750 (HV207_29760) | 62106..62702 | + | 597 | WP_075606926 | conjugal transfer pilus-stabilizing protein TraP | - |
HV207_RS29755 (HV207_29765) | 62725..63294 | + | 570 | WP_075606925 | type IV conjugative transfer system lipoprotein TraV | traV |
HV207_RS29760 (HV207_29770) | 63406..63684 | + | 279 | WP_053390295 | hypothetical protein | - |
HV207_RS29765 (HV207_29775) | 63691..63879 | + | 189 | WP_053390294 | hypothetical protein | - |
HV207_RS30510 | 63963..64364 | + | 402 | WP_064343532 | hypothetical protein | - |
HV207_RS29775 (HV207_29785) | 64361..64660 | + | 300 | WP_053390308 | hypothetical protein | - |
HV207_RS29780 (HV207_29790) | 64748..65131 | + | 384 | WP_042946290 | hypothetical protein | - |
HV207_RS29785 (HV207_29795) | 65224..67863 | + | 2640 | WP_064343533 | type IV secretion system protein TraC | virb4 |
HV207_RS29790 (HV207_29800) | 67863..68255 | + | 393 | WP_064343534 | type-F conjugative transfer system protein TrbI | - |
HV207_RS29795 (HV207_29805) | 68252..68878 | + | 627 | WP_064343535 | type-F conjugative transfer system protein TraW | traW |
HV207_RS29800 (HV207_29810) | 68922..69881 | + | 960 | WP_229295972 | conjugal transfer pilus assembly protein TraU | traU |
HV207_RS29805 (HV207_29815) | 69894..70520 | + | 627 | WP_181247950 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
HV207_RS29810 (HV207_29820) | 70517..72472 | + | 1956 | WP_102016187 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
HV207_RS29815 (HV207_29825) | 72504..72764 | + | 261 | WP_181247951 | hypothetical protein | - |
HV207_RS29820 (HV207_29830) | 72757..73368 | + | 612 | WP_060415486 | hypothetical protein | - |
HV207_RS29825 (HV207_29835) | 73358..73600 | + | 243 | WP_060415487 | conjugal transfer protein TrbE | - |
HV207_RS29830 (HV207_29840) | 73646..73972 | + | 327 | WP_060415488 | hypothetical protein | - |
HV207_RS29835 (HV207_29845) | 73993..74745 | + | 753 | WP_060415489 | type-F conjugative transfer system pilin assembly protein TraF | traF |
HV207_RS29840 (HV207_29850) | 74756..74992 | + | 237 | WP_032736848 | type-F conjugative transfer system pilin chaperone TraQ | - |
HV207_RS29845 (HV207_29855) | 75006..75542 | + | 537 | WP_180807780 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
HV207_RS29850 (HV207_29860) | 75529..76899 | + | 1371 | WP_102016205 | conjugal transfer pilus assembly protein TraH | traH |
HV207_RS29855 (HV207_29865) | 76899..79724 | + | 2826 | WP_060415492 | conjugal transfer mating-pair stabilization protein TraG | traG |
HV207_RS29860 (HV207_29870) | 79741..80412 | + | 672 | WP_088883382 | hypothetical protein | - |
HV207_RS29865 (HV207_29875) | 80659..81351 | + | 693 | WP_162762153 | hypothetical protein | - |
HV207_RS29870 (HV207_29880) | 81372..81635 | + | 264 | WP_142372547 | hypothetical protein | - |
HV207_RS29875 (HV207_29885) | 81706..84006 | + | 2301 | WP_181247952 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 5684 | GenBank | NZ_CP055512 |
Plasmid name | pRHBSTW-00472_4 | Incompatibility group | IncFII |
Plasmid size | 95633 bp | Coordinate of oriT [Strand] | 55455..55503 [-] |
Host baterium | Klebsiella pneumoniae strain RHBSTW-00472 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |