Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105238
Name   oriT_pRHBSTW-00510_4 in_silico
Organism   Klebsiella pneumoniae strain RHBSTW-00510
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP056435 (102048..102097 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pRHBSTW-00510_4
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3599 GenBank   WP_004153015
Name   traI_HV225_RS28675_pRHBSTW-00510_4 insolico UniProt ID   A0A8A5TN24
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190492.51 Da        Isoelectric Point: 6.3661

>WP_004153015.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

DNA helicase TraI (TraI_C)

(1433-1555)

TrwC relaxase (TrwC)

(10-284)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

AAA domain (AAA_30)

(971-1157)


  Protein structure



No available structure.




T4CP

ID   3559 GenBank   WP_228728115
Name   traD_HV225_RS28680_pRHBSTW-00510_4 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85870.90 Da        Isoelectric Point: 5.1165

>WP_228728115.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNKDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 73658..102655

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV225_RS28680 (HV225_28680) 73658..75970 - 2313 WP_228728115 type IV conjugative transfer system coupling protein TraD virb4
HV225_RS28685 (HV225_28685) 76099..76788 - 690 WP_004198206 hypothetical protein -
HV225_RS28690 (HV225_28690) 76980..77711 - 732 WP_004152622 conjugal transfer complement resistance protein TraT -
HV225_RS28695 (HV225_28695) 77895..78443 - 549 WP_004152623 conjugal transfer entry exclusion protein TraS -
HV225_RS28700 (HV225_28700) 78456..81305 - 2850 WP_004152624 conjugal transfer mating-pair stabilization protein TraG traG
HV225_RS28705 (HV225_28705) 81305..82684 - 1380 WP_011977731 conjugal transfer pilus assembly protein TraH traH
HV225_RS28710 (HV225_28710) 82662..83105 - 444 WP_004152679 F-type conjugal transfer protein TrbF -
HV225_RS28715 (HV225_28715) 83151..83708 - 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HV225_RS28720 (HV225_28720) 83680..83919 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
HV225_RS28725 (HV225_28725) 83930..84682 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
HV225_RS28730 (HV225_28730) 84703..85029 - 327 WP_004152676 hypothetical protein -
HV225_RS28735 (HV225_28735) 85042..85290 - 249 WP_004152675 hypothetical protein -
HV225_RS28740 (HV225_28740) 85268..85522 - 255 WP_004152674 conjugal transfer protein TrbE -
HV225_RS28745 (HV225_28745) 85554..87509 - 1956 WP_004152673 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HV225_RS28750 (HV225_28750) 87568..88206 - 639 WP_011977786 type-F conjugative transfer system pilin assembly protein TrbC trbC
HV225_RS28755 (HV225_28755) 88219..89208 - 990 WP_011977785 conjugal transfer pilus assembly protein TraU traU
HV225_RS28760 (HV225_28760) 89205..89594 - 390 WP_004152508 hypothetical protein -
HV225_RS28765 (HV225_28765) 89636..90262 - 627 WP_004152507 type-F conjugative transfer system protein TraW traW
HV225_RS28770 (HV225_28770) 90262..90651 - 390 WP_004152506 type-F conjugative transfer system protein TrbI -
HV225_RS28775 (HV225_28775) 90651..93290 - 2640 WP_004152505 type IV secretion system protein TraC virb4
HV225_RS28780 (HV225_28780) 93362..93760 - 399 WP_011977783 hypothetical protein -
HV225_RS28785 (HV225_28785) 93768..94157 - 390 WP_004153076 hypothetical protein -
HV225_RS28790 (HV225_28790) 94200..94604 - 405 WP_004152503 hypothetical protein -
HV225_RS28795 (HV225_28795) 94671..94982 - 312 WP_004152502 hypothetical protein -
HV225_RS28800 (HV225_28800) 94983..95201 - 219 WP_004152501 hypothetical protein -
HV225_RS28805 (HV225_28805) 95307..95717 - 411 WP_004152499 hypothetical protein -
HV225_RS28810 (HV225_28810) 95849..96433 - 585 WP_004161368 type IV conjugative transfer system lipoprotein TraV traV
HV225_RS28815 (HV225_28815) 96547..97971 - 1425 WP_004155033 F-type conjugal transfer pilus assembly protein TraB traB
HV225_RS28820 (HV225_28820) 97971..98711 - 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
HV225_RS28825 (HV225_28825) 98698..99264 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
HV225_RS28830 (HV225_28830) 99284..99589 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
HV225_RS28835 (HV225_28835) 99603..99971 - 369 WP_004152496 type IV conjugative transfer system pilin TraA -
HV225_RS28840 (HV225_28840) 100025..100411 - 387 WP_004152495 TraY domain-containing protein -
HV225_RS28845 (HV225_28845) 100490..101176 - 687 WP_004152494 transcriptional regulator TraJ family protein -
HV225_RS28850 (HV225_28850) 101350..101748 - 399 WP_004152493 conjugal transfer relaxosome DNA-binding protein TraM -
HV225_RS28855 (HV225_28855) 102170..102655 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
HV225_RS30300 102688..102789 - 102 Protein_113 hypothetical protein -
HV225_RS28865 (HV225_28865) 104431..104691 - 261 Protein_115 hypothetical protein -
HV225_RS28870 (HV225_28870) 104724..105545 - 822 WP_004152492 DUF932 domain-containing protein -
HV225_RS28875 (HV225_28875) 106365..107198 - 834 WP_004152751 N-6 DNA methylase -
HV225_RS28880 (HV225_28880) 107249..107395 - 147 WP_004152750 hypothetical protein -

Host bacterium

ID   5676 GenBank   NZ_CP056435
Plasmid name   pRHBSTW-00510_4 Incompatibility group   IncFIB
Plasmid size   136301 bp Coordinate of oriT [Strand]   102048..102097 [+]
Host baterium   Klebsiella pneumoniae strain RHBSTW-00510

Cargo genes

Drug resistance gene   qnrB1, dfrA14, blaCTX-M-15, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, sul2
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9