Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 105200 |
Name | oriT_pRHBSTW-00113_3 |
Organism | Klebsiella pneumoniae strain RHBSTW-00113 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP056856 (75388..75437 [-], 50 nt) |
oriT length | 50 nt |
IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
Location of nic site | 33..34 |
Conserved sequence flanking the nic site |
TGTGTGGTGA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pRHBSTW-00113_3
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 3584 | GenBank | WP_075606914 |
Name | traI_HV075_RS26645_pRHBSTW-00113_3 | UniProt ID | A0A6M3HIK1 |
Length | 1752 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190386.65 Da Isoelectric Point: 5.5084
>WP_075606914.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 1600 | GenBank | WP_053390193 |
Name | WP_053390193_pRHBSTW-00113_3 | UniProt ID | A0A8G2A327 |
Length | 130 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 130 a.a. Molecular weight: 14883.89 Da Isoelectric Point: 4.3779
>WP_053390193.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 3545 | GenBank | WP_075606915 |
Name | traD_HV075_RS26640_pRHBSTW-00113_3 | UniProt ID | _ |
Length | 772 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 772 a.a. Molecular weight: 86712.47 Da Isoelectric Point: 5.0825
>WP_075606915.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 74821..104099
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HV075_RS26435 (HV075_26440) | 70011..70886 | + | 876 | WP_060415463 | DUF4942 domain-containing protein | - |
HV075_RS26440 (HV075_26445) | 71666..72004 | + | 339 | WP_020804855 | hypothetical protein | - |
HV075_RS26445 (HV075_26450) | 72061..72408 | + | 348 | WP_064182675 | hypothetical protein | - |
HV075_RS26450 (HV075_26455) | 72497..72727 | + | 231 | WP_060415465 | hypothetical protein | - |
HV075_RS26455 (HV075_26460) | 72774..73607 | + | 834 | WP_060415466 | N-6 DNA methylase | - |
HV075_RS26460 (HV075_26465) | 73893..74261 | - | 369 | WP_080848162 | hypothetical protein | - |
HV075_RS26465 (HV075_26470) | 74256..74798 | + | 543 | WP_060415467 | antirestriction protein | - |
HV075_RS26470 (HV075_26475) | 74821..75243 | - | 423 | WP_073972322 | transglycosylase SLT domain-containing protein | virB1 |
HV075_RS26475 (HV075_26480) | 75749..76141 | + | 393 | WP_053390193 | conjugal transfer relaxosome DNA-binding protein TraM | - |
HV075_RS26480 (HV075_26485) | 76379..77080 | + | 702 | WP_094898581 | hypothetical protein | - |
HV075_RS26485 (HV075_26490) | 77241..77402 | + | 162 | WP_172686697 | TraY domain-containing protein | - |
HV075_RS26490 (HV075_26495) | 77467..77835 | + | 369 | WP_064386081 | type IV conjugative transfer system pilin TraA | - |
HV075_RS26495 (HV075_26500) | 77849..78154 | + | 306 | WP_064343526 | type IV conjugative transfer system protein TraL | traL |
HV075_RS26500 (HV075_26505) | 78170..78736 | + | 567 | WP_064359954 | type IV conjugative transfer system protein TraE | traE |
HV075_RS26505 (HV075_26510) | 78723..79457 | + | 735 | WP_064343528 | type-F conjugative transfer system secretin TraK | traK |
HV075_RS26510 (HV075_26515) | 79457..80878 | + | 1422 | WP_053390199 | F-type conjugal transfer pilus assembly protein TraB | traB |
HV075_RS26515 (HV075_26520) | 80871..81305 | + | 435 | WP_020322358 | conjugal transfer protein TraP | - |
HV075_RS26520 (HV075_26525) | 81339..81923 | + | 585 | WP_064343530 | type IV conjugative transfer system lipoprotein TraV | traV |
HV075_RS26525 (HV075_26530) | 82536..82724 | + | 189 | WP_053390286 | hypothetical protein | - |
HV075_RS27565 | 82808..83209 | + | 402 | WP_064343532 | hypothetical protein | - |
HV075_RS26535 (HV075_26540) | 83206..83505 | + | 300 | WP_053390308 | hypothetical protein | - |
HV075_RS26540 (HV075_26545) | 83593..83976 | + | 384 | WP_042946290 | hypothetical protein | - |
HV075_RS26545 (HV075_26550) | 84069..86708 | + | 2640 | WP_064343533 | type IV secretion system protein TraC | virb4 |
HV075_RS26550 (HV075_26555) | 86708..87100 | + | 393 | WP_064343534 | type-F conjugative transfer system protein TrbI | - |
HV075_RS26555 (HV075_26560) | 87097..87723 | + | 627 | WP_064343535 | type-F conjugative transfer system protein TraW | traW |
HV075_RS26560 (HV075_26565) | 87767..88726 | + | 960 | WP_229295972 | conjugal transfer pilus assembly protein TraU | traU |
HV075_RS26565 (HV075_26570) | 88739..89386 | + | 648 | WP_064343536 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
HV075_RS26570 (HV075_26575) | 89383..89766 | + | 384 | WP_053390297 | hypothetical protein | - |
HV075_RS26575 (HV075_26580) | 89763..91718 | + | 1956 | WP_064343537 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
HV075_RS26580 (HV075_26585) | 91750..92010 | + | 261 | WP_053390216 | hypothetical protein | - |
HV075_RS26585 (HV075_26590) | 92003..92614 | + | 612 | WP_053390217 | hypothetical protein | - |
HV075_RS26590 (HV075_26595) | 92604..92846 | + | 243 | WP_053390218 | conjugal transfer protein TrbE | - |
HV075_RS26595 (HV075_26600) | 92892..93218 | + | 327 | WP_053390219 | hypothetical protein | - |
HV075_RS26600 (HV075_26605) | 93239..93991 | + | 753 | WP_053390220 | type-F conjugative transfer system pilin assembly protein TraF | traF |
HV075_RS26605 (HV075_26610) | 94002..94238 | + | 237 | WP_053390221 | type-F conjugative transfer system pilin chaperone TraQ | - |
HV075_RS26610 (HV075_26615) | 94213..94773 | + | 561 | WP_039698500 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
HV075_RS26615 (HV075_26620) | 94760..96130 | + | 1371 | WP_075606918 | conjugal transfer pilus assembly protein TraH | traH |
HV075_RS26620 (HV075_26625) | 96130..99009 | + | 2880 | WP_064386194 | conjugal transfer mating-pair stabilization protein TraG | traG |
HV075_RS26625 (HV075_26630) | 99026..99694 | + | 669 | WP_128317877 | hypothetical protein | - |
HV075_RS26630 (HV075_26635) | 100047..100778 | + | 732 | WP_064386198 | conjugal transfer complement resistance protein TraT | - |
HV075_RS27570 | 100970..101662 | + | 693 | WP_202395549 | hypothetical protein | - |
HV075_RS26640 (HV075_26645) | 101781..104099 | + | 2319 | WP_075606915 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 5638 | GenBank | NZ_CP056856 |
Plasmid name | pRHBSTW-00113_3 | Incompatibility group | IncN |
Plasmid size | 115230 bp | Coordinate of oriT [Strand] | 75388..75437 [-] |
Host baterium | Klebsiella pneumoniae strain RHBSTW-00113 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | merR2, merB, merD, merE |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |