Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105155
Name   oriT_pRHBSTW-00002_2 in_silico
Organism   Enterobacter roggenkampii strain RHBSTW-00002
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP058197 (137792..137840 [-], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_pRHBSTW-00002_2
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3563 GenBank   WP_040118495
Name   traI_HV027_RS22780_pRHBSTW-00002_2 insolico UniProt ID   A0A7H9GIQ2
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190247.71 Da        Isoelectric Point: 6.0516

>WP_040118495.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVTGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLAGTRVQKDGVSETVLTGNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENLLANRIALGKIYQSALRADIES
MGYKTVDAGKHGMWEMKDVPVEAFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADAHAAELARAPAAPVKTDGPDIADVVTRAIAGLSDRKVQFTYADVLARTVGQLEA
KPGVFELARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEVQRQNHVSVTPDASVMREKPF
SDAVSVLAQDRPAMGIVAGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGRQATADIISEPDKSVRYSRLAQEFAVSVREGQESVAQISGTREQNVLNNVIRDTLKNEGALGNRDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPEARKHDRFVIDRVTASSNMLTLKDREGERLDLKVSVVD
SQWTLFRTDKLPVAEGERLAVLGKIPDTRLKGGESVTVLKAEAGQLTVQRPGQKTTQTLPVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQDAARTTEKLARHTAFSVVSEQ
LKVRSGEADLEAAIAQQKAGLHTPAEQAIHLSVPLLESNSLTFTRTQLLATALETGGGKVPMTDIDATIQ
AQIKAGSLLNVPVASGHGNDLLISRQTWDAEKSVLTRVLEGKDAMTPLMDRVPGSLMTDLTAGQRAATRM
ILETPDRFTVVQGYAGVGKTTQFKSVMSAISLLPEEARSRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLQQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDISRALSTIEQVTPEQVPRKDGAWVPENSVTEFTPAQEKAIQD
ALKEGKEIPPDTPASLHDAIVKDYTGRTPAAQASTLIITHLNADRRALNGLIHDARVQNGEVSKEEVTLP
VLVTSNIRDGELRKLSTWSEHQGAVALLDNTYQRIESVDKENQLITLKDNEGNIRLLSPREASAEGVTLY
RPEHITVSQGDRMRFSKSDPERGYVANSVWEVKAFSGDSVTLTDGKTTRTLNPKADEAQRHIDLAYAITA
HGAQGASEPFAIALEGVEGRRKALVSFESAYVALSRMKQHAQVYTDNREGWTTAVSRSQEKASAHDVLEP
QNARAVRNAGQLYGRAQPLDETAAGRAALQQSGLARGRSPGKFISPGKKYPQPHVALPAFDKNGKEAGIW
LSPLTDRDGRLEAIGGEGRIMGDETARFVALQNSRNGESLLAGNMGEGVRAARNHPDSGVIVRLAGDDRP
WNPEAITGGRVWADPLPNTPLPDTGTDIPLPPDVLAQRAAEEVQRREMEKQAEQTAREVAGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTLLPDDPQTRRQEAAIQQVASESLQRERLQQMERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




Auxiliary protein

ID   1591 GenBank   WP_020804860
Name   WP_020804860_pRHBSTW-00002_2 insolico UniProt ID   A0A7H9GLQ1
Length   130 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 130 a.a.        Molecular weight: 15022.07 Da        Isoelectric Point: 4.5248

>WP_020804860.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMRTFFPDEQEEADE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure


Source ID Structure
AlphaFold DB A0A7H9GLQ1


T4CP

ID   3528 GenBank   WP_040118494
Name   traD_HV027_RS22775_pRHBSTW-00002_2 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 85764.48 Da        Isoelectric Point: 4.8107

>WP_040118494.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
EIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKDVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRCYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRTLDTRVDARLSALLEAREAEGNLARALFTPDAPEPEQRDADSRTSPGQPEPENAP
VSPAPVKSASTAETLAAPPSVKASEAPQATVTTVPLTRPAPATTPAASSATASSVAATAATTGGTEQELA
QQPADTGQEVMPAGMNEDGEIEDMQAYDTWLADEQTQREMQRREEVNINHSHRRDEQDDIEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 141324..165905

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV027_RS22590 (HV027_22600) 136660..137202 + 543 WP_040118470 antirestriction protein -
HV027_RS22595 (HV027_22605) 137225..137647 - 423 WP_051800693 transglycosylase SLT domain-containing protein -
HV027_RS22600 (HV027_22610) 138152..138544 + 393 WP_020804860 conjugal transfer relaxosome DNA-binding protein TraM -
HV027_RS22605 (HV027_22615) 138782..139474 + 693 WP_032426082 conjugal transfer protein TrbJ -
HV027_RS22610 (HV027_22620) 139649..139813 + 165 WP_032426810 TraY domain-containing protein -
HV027_RS22615 (HV027_22625) 139876..139980 + 105 Protein_163 type IV conjugative transfer system pilin TraA -
HV027_RS22620 (HV027_22630) 140014..140982 + 969 WP_074132802 IS5 family transposase -
HV027_RS22625 (HV027_22635) 141026..141310 + 285 WP_181239554 type IV conjugative transfer system pilin TraA -
HV027_RS22630 (HV027_22640) 141324..141629 + 306 WP_020323523 type IV conjugative transfer system protein TraL traL
HV027_RS22635 (HV027_22645) 141649..142215 + 567 WP_040118472 type IV conjugative transfer system protein TraE traE
HV027_RS22640 (HV027_22650) 142202..142942 + 741 WP_040118473 type-F conjugative transfer system secretin TraK traK
HV027_RS22645 (HV027_22655) 142942..144357 + 1416 WP_040118474 F-type conjugal transfer pilus assembly protein TraB traB
HV027_RS22650 (HV027_22660) 144350..144946 + 597 WP_117146400 conjugal transfer protein TraP -
HV027_RS23815 144927..145163 + 237 WP_244058567 hypothetical protein -
HV027_RS22660 (HV027_22670) 145186..145767 + 582 WP_040118475 type IV conjugative transfer system lipoprotein TraV traV
HV027_RS22665 (HV027_22675) 145914..146117 + 204 WP_020315141 hypothetical protein -
HV027_RS22670 (HV027_22680) 146358..146771 + 414 WP_040118476 hypothetical protein -
HV027_RS22675 (HV027_22685) 146800..147099 + 300 WP_040118477 hypothetical protein -
HV027_RS22680 (HV027_22690) 147116..147340 + 225 WP_040118478 hypothetical protein -
HV027_RS22685 (HV027_22695) 147337..147741 + 405 WP_040118479 hypothetical protein -
HV027_RS22690 (HV027_22700) 147874..150513 + 2640 WP_040118480 type IV secretion system protein TraC virb4
HV027_RS22695 (HV027_22705) 150546..150947 + 402 WP_051800694 type-F conjugative transfer system protein TrbI -
HV027_RS22700 (HV027_22710) 150944..151570 + 627 WP_040118481 type-F conjugative transfer system protein TraW traW
HV027_RS22705 (HV027_22715) 151590..152573 + 984 WP_074423225 conjugal transfer pilus assembly protein TraU traU
HV027_RS22710 (HV027_22720) 152586..153203 + 618 WP_040118483 type-F conjugative transfer system pilin assembly protein TrbC trbC
HV027_RS22715 (HV027_22725) 153200..155080 + 1881 WP_117146399 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HV027_RS22720 (HV027_22730) 155118..155399 + 282 WP_040118485 hypothetical protein -
HV027_RS22725 (HV027_22735) 155389..155616 + 228 WP_040118486 conjugal transfer protein TrbE -
HV027_RS22730 (HV027_22740) 155627..155953 + 327 WP_040118487 hypothetical protein -
HV027_RS22735 (HV027_22745) 155972..156724 + 753 WP_040118488 type-F conjugative transfer system pilin assembly protein TraF traF
HV027_RS22740 (HV027_22750) 156735..156974 + 240 WP_040118489 type-F conjugative transfer system pilin chaperone TraQ -
HV027_RS22745 (HV027_22755) 156946..157521 + 576 WP_040118490 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HV027_RS22750 (HV027_22760) 157508..158878 + 1371 WP_040118491 conjugal transfer pilus assembly protein TraH traH
HV027_RS22755 (HV027_22765) 158878..161526 + 2649 Protein_191 conjugal transfer mating-pair stabilization protein TraG -
HV027_RS22760 (HV027_22770) 161584..162552 - 969 WP_074132802 IS5 family transposase -
HV027_RS22765 (HV027_22775) 162647..162817 + 171 WP_181239545 hypothetical protein -
HV027_RS22770 (HV027_22780) 162828..163361 + 534 WP_040118493 hypothetical protein -
HV027_RS22775 (HV027_22785) 163605..165905 + 2301 WP_040118494 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   5593 GenBank   NZ_CP058197
Plasmid name   pRHBSTW-00002_2 Incompatibility group   IncFII
Plasmid size   176631 bp Coordinate of oriT [Strand]   137792..137840 [-]
Host baterium   Enterobacter roggenkampii strain RHBSTW-00002

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -