Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105094 |
| Name | oriT_pKP20194b-p3  |
| Organism | Klebsiella pneumoniae strain KP20194b |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP054771 (79900..79981 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pKP20194b-p3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3547 | GenBank | WP_032440569 |
| Name | Relaxase_HUS00_RS29220_pKP20194b-p3 |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 3515 | GenBank | WP_032440593 |
| Name | trbC_HUS00_RS28870_pKP20194b-p3 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1995..23928
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUS00_RS28760 (HUS00_28765) | 70..297 | + | 228 | WP_032440573 | hypothetical protein | - |
| HUS00_RS28765 (HUS00_28770) | 1029..1508 | + | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| HUS00_RS28770 (HUS00_28775) | 1534..1998 | + | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| HUS00_RS28775 (HUS00_28780) | 1995..2813 | + | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| HUS00_RS28780 (HUS00_28785) | 2791..3978 | + | 1188 | WP_077255878 | plasmid transfer ATPase TraJ | - |
| HUS00_RS28785 (HUS00_28790) | 3975..4241 | + | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| HUS00_RS28790 (HUS00_28795) | 4259..7672 | + | 3414 | WP_052145516 | LPD7 domain-containing protein | - |
| HUS00_RS28795 (HUS00_28800) | 7706..8059 | + | 354 | WP_032440584 | hypothetical protein | traL |
| HUS00_RS28800 (HUS00_28805) | 8131..8799 | + | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| HUS00_RS28805 (HUS00_28810) | 8810..9913 | + | 1104 | WP_077264204 | DotH/IcmK family type IV secretion protein | traN |
| HUS00_RS28810 (HUS00_28815) | 9917..11257 | + | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| HUS00_RS28815 (HUS00_28820) | 11264..11983 | + | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| HUS00_RS28820 (HUS00_28825) | 11993..12526 | + | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| HUS00_RS28825 (HUS00_28830) | 12582..12983 | + | 402 | WP_032440588 | DUF6750 family protein | traR |
| HUS00_RS28830 (HUS00_28835) | 13027..13590 | + | 564 | WP_045325070 | hypothetical protein | traT |
| HUS00_RS28835 (HUS00_28840) | 13600..16647 | + | 3048 | WP_032440589 | conjugal transfer protein | traU |
| HUS00_RS28840 (HUS00_28845) | 16657..17853 | + | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| HUS00_RS28845 (HUS00_28850) | 17850..18431 | + | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| HUS00_RS28850 (HUS00_28855) | 18473..20617 | + | 2145 | WP_143458320 | DotA/TraY family protein | traY |
| HUS00_RS28855 (HUS00_28860) | 20683..21306 | + | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HUS00_RS28860 (HUS00_28865) | 21408..22826 | + | 1419 | WP_063666705 | hypothetical protein | trbA |
| HUS00_RS28865 (HUS00_28870) | 22837..23928 | + | 1092 | WP_050597336 | thioredoxin fold domain-containing protein | trbB |
| HUS00_RS28870 (HUS00_28875) | 23930..26122 | + | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| HUS00_RS28875 (HUS00_28880) | 26168..26701 | + | 534 | WP_032440594 | phospholipase D family protein | - |
| HUS00_RS28880 (HUS00_28885) | 26713..26964 | + | 252 | WP_113394424 | hypothetical protein | - |
| HUS00_RS28885 (HUS00_28890) | 27470..28165 | + | 696 | WP_254910806 | RepA family replication protein | - |
Host bacterium
| ID | 5532 | GenBank | NZ_CP054771 |
| Plasmid name | pKP20194b-p3 | Incompatibility group | IncFII |
| Plasmid size | 87095 bp | Coordinate of oriT [Strand] | 79900..79981 [+] |
| Host baterium | Klebsiella pneumoniae strain KP20194b |
Cargo genes
| Drug resistance gene | dfrA14, sul2, catA2, tet(A), blaLAP-2, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |