Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105051 |
| Name | oriT_pKP20194b2-p3  |
| Organism | Klebsiella pneumoniae strain KP20194b2 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP054765 (51784..51865 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pKP20194b2-p3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3535 | GenBank | WP_032440569 |
| Name | Relaxase_HUS01_RS29015_pKP20194b2-p3 |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 3502 | GenBank | WP_032440593 |
| Name | trbC_HUS01_RS28880_pKP20194b2-p3 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20742..42675
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUS01_RS28865 (HUS01_28870) | 16505..17200 | - | 696 | WP_254910806 | RepA family replication protein | - |
| HUS01_RS28870 (HUS01_28875) | 17706..17957 | - | 252 | WP_113394424 | hypothetical protein | - |
| HUS01_RS28875 (HUS01_28880) | 17969..18502 | - | 534 | WP_032440594 | phospholipase D family protein | - |
| HUS01_RS28880 (HUS01_28885) | 18548..20740 | - | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| HUS01_RS28885 (HUS01_28890) | 20742..21833 | - | 1092 | WP_050597336 | thioredoxin fold domain-containing protein | trbB |
| HUS01_RS28890 (HUS01_28895) | 21844..23262 | - | 1419 | WP_063666705 | hypothetical protein | trbA |
| HUS01_RS28895 (HUS01_28900) | 23364..23987 | - | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HUS01_RS28900 (HUS01_28905) | 24053..26197 | - | 2145 | WP_143458320 | DotA/TraY family protein | traY |
| HUS01_RS28905 (HUS01_28910) | 26239..26820 | - | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| HUS01_RS28910 (HUS01_28915) | 26817..28013 | - | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| HUS01_RS28915 (HUS01_28920) | 28023..31070 | - | 3048 | WP_032440589 | conjugal transfer protein | traU |
| HUS01_RS28920 (HUS01_28925) | 31080..31643 | - | 564 | WP_045325070 | hypothetical protein | traT |
| HUS01_RS28925 (HUS01_28930) | 31687..32088 | - | 402 | WP_032440588 | DUF6750 family protein | traR |
| HUS01_RS28930 (HUS01_28935) | 32144..32677 | - | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| HUS01_RS28935 (HUS01_28940) | 32687..33406 | - | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| HUS01_RS28940 (HUS01_28945) | 33413..34753 | - | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| HUS01_RS28945 (HUS01_28950) | 34757..35860 | - | 1104 | WP_077264204 | DotH/IcmK family type IV secretion protein | traN |
| HUS01_RS28950 (HUS01_28955) | 35871..36539 | - | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| HUS01_RS28955 (HUS01_28960) | 36611..36964 | - | 354 | WP_032440584 | hypothetical protein | traL |
| HUS01_RS28960 (HUS01_28965) | 36998..40411 | - | 3414 | WP_052145516 | LPD7 domain-containing protein | - |
| HUS01_RS28965 (HUS01_28970) | 40429..40695 | - | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| HUS01_RS28970 (HUS01_28975) | 40692..41879 | - | 1188 | WP_077255878 | plasmid transfer ATPase TraJ | - |
| HUS01_RS28975 (HUS01_28980) | 41857..42675 | - | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| HUS01_RS28980 (HUS01_28985) | 42672..43136 | - | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| HUS01_RS28985 (HUS01_28990) | 43162..43641 | - | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| HUS01_RS28990 (HUS01_28995) | 44373..44600 | - | 228 | WP_032440573 | hypothetical protein | - |
| HUS01_RS28995 (HUS01_29000) | 45210..46052 | + | 843 | WP_032440572 | helix-turn-helix domain-containing protein | - |
| HUS01_RS29000 (HUS01_29005) | 46112..47041 | + | 930 | WP_032440581 | hypothetical protein | - |
| HUS01_RS29005 (HUS01_29010) | 47034..47528 | + | 495 | WP_032440571 | molybdopterin-dependent oxidoreductase | - |
Host bacterium
| ID | 5489 | GenBank | NZ_CP054765 |
| Plasmid name | pKP20194b2-p3 | Incompatibility group | IncFII |
| Plasmid size | 87095 bp | Coordinate of oriT [Strand] | 51784..51865 [-] |
| Host baterium | Klebsiella pneumoniae strain KP20194b2 |
Cargo genes
| Drug resistance gene | sul2, dfrA14, qnrS1, blaLAP-2, tet(A), catA2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |