Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105038 |
| Name | oriT_pC2974-3  |
| Organism | Klebsiella pneumoniae strain C2974 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP039797 (83001..84868 [+], 1868 nt) |
| oriT length | 1868 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 1868 nt
>oriT_pC2974-3
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAAATAGGACAGAATATCTGTAGCCATGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCCTTTGACCCTGGGGATTTTGGCGCGGCGTTCAGCCACGACATTACCCCCATGCGGCAGGCAATAAGGGCGTCCAGCCCGCATGTACTGGCGCACGCCGTTTCGTGCAGGGAAGCATGCTTTCCTGCACATGGTCGCAACGCGGCCACCTTCTTCGCCCCAGACACTCGCCCCATATTTTCGCCCGACACCATGGGGCGAATTGATGGGGCGATGTCATGGGGCGAGTAGCTGACTAAGGAGGCATCATGATTTTAAATAAGC
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAAATAGGACAGAATATCTGTAGCCATGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCCTTTGACCCTGGGGATTTTGGCGCGGCGTTCAGCCACGACATTACCCCCATGCGGCAGGCAATAAGGGCGTCCAGCCCGCATGTACTGGCGCACGCCGTTTCGTGCAGGGAAGCATGCTTTCCTGCACATGGTCGCAACGCGGCCACCTTCTTCGCCCCAGACACTCGCCCCATATTTTCGCCCGACACCATGGGGCGAATTGATGGGGCGATGTCATGGGGCGAGTAGCTGACTAAGGAGGCATCATGATTTTAAATAAGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3489 | GenBank | WP_094965467 |
| Name | t4cp2_FBF58_RS27695_pC2974-3 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103112.07 Da Isoelectric Point: 7.4625
>WP_094965467.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacteriaceae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAVLFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAVLFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 3490 | GenBank | WP_063116534 |
| Name | t4cp2_FBF58_RS27755_pC2974-3 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69595.85 Da Isoelectric Point: 6.2735
>WP_063116534.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 73143..83068
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FBF58_RS27650 | 69677..70603 | + | 927 | WP_000039782 | hypothetical protein | - |
| FBF58_RS27655 (FBF58_27755) | 70743..70928 | + | 186 | WP_000205185 | AlpA family transcriptional regulator | - |
| FBF58_RS27660 (FBF58_27760) | 70950..71132 | + | 183 | WP_001513329 | hypothetical protein | - |
| FBF58_RS27665 (FBF58_27765) | 71117..71698 | + | 582 | WP_001593435 | DUF2857 domain-containing protein | - |
| FBF58_RS27670 (FBF58_27770) | 71688..71897 | + | 210 | WP_001112403 | TraR/DksA family transcriptional regulator | - |
| FBF58_RS27675 (FBF58_27775) | 72119..72268 | + | 150 | Protein_54 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| FBF58_RS27680 (FBF58_27780) | 72837..73070 | + | 234 | WP_001231171 | hypothetical protein | - |
| FBF58_RS27685 (FBF58_27785) | 73143..73853 | + | 711 | WP_001593436 | lytic transglycosylase domain-containing protein | virB1 |
| FBF58_RS27690 (FBF58_27790) | 73853..74146 | + | 294 | WP_000600171 | TrbC/VirB2 family protein | virB2 |
| FBF58_RS27695 (FBF58_27795) | 74159..76897 | + | 2739 | WP_094965467 | VirB3 family type IV secretion system protein | virb4 |
| FBF58_RS27700 (FBF58_27800) | 76915..77622 | + | 708 | WP_000857358 | type IV secretion system protein | - |
| FBF58_RS27705 (FBF58_27805) | 77630..77872 | + | 243 | WP_001593437 | EexN family lipoprotein | - |
| FBF58_RS27710 (FBF58_27810) | 77876..78949 | + | 1074 | WP_001593439 | type IV secretion system protein | virB6 |
| FBF58_RS27720 (FBF58_27815) | 79171..79854 | + | 684 | WP_000003229 | type IV secretion system protein | virB8 |
| FBF58_RS27725 (FBF58_27820) | 79851..80759 | + | 909 | WP_000976328 | P-type conjugative transfer protein VirB9 | virB9 |
| FBF58_RS27730 (FBF58_27825) | 80803..82053 | + | 1251 | WP_023339226 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| FBF58_RS27735 (FBF58_27830) | 82043..83068 | + | 1026 | WP_000125558 | P-type DNA transfer ATPase VirB11 | virB11 |
| FBF58_RS27740 (FBF58_27835) | 83065..83463 | + | 399 | WP_000592251 | cag pathogenicity island Cag12 family protein | - |
| FBF58_RS27745 (FBF58_27840) | 83499..83804 | + | 306 | WP_000754360 | hypothetical protein | - |
| FBF58_RS29780 (FBF58_27845) | 83892..84143 | + | 252 | WP_087205727 | dpoa decarboxylase | - |
| FBF58_RS27755 (FBF58_27860) | 84853..86712 | + | 1860 | WP_063116534 | type IV secretion system DNA-binding domain-containing protein | - |
| FBF58_RS27760 (FBF58_27865) | 86722..87468 | + | 747 | WP_021551854 | MobC family replication-relaxation protein | - |
Host bacterium
| ID | 5476 | GenBank | NZ_CP039797 |
| Plasmid name | pC2974-3 | Incompatibility group | - |
| Plasmid size | 114658 bp | Coordinate of oriT [Strand] | 83001..84868 [+] |
| Host baterium | Klebsiella pneumoniae strain C2974 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |