Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105033 |
| Name | oriT_p50595_IncFII  |
| Organism | Klebsiella pneumoniae strain 50595 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP050373 (58673..58722 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_p50595_IncFII
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3522 | GenBank | WP_015632446 |
| Name | Relaxase_HB637_RS27835_p50595_IncFII |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 3486 | GenBank | WP_015632442 |
| Name | trbC_HB637_RS27870_p50595_IncFII |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 58115..71737
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HB637_RS27640 (HB637_27650) | 53132..53482 | + | 351 | WP_004152758 | hypothetical protein | - |
| HB637_RS27655 (HB637_27665) | 54118..54474 | + | 357 | WP_004152717 | hypothetical protein | - |
| HB637_RS27660 (HB637_27670) | 54535..54747 | + | 213 | WP_004152718 | hypothetical protein | - |
| HB637_RS27665 (HB637_27675) | 54758..54982 | + | 225 | WP_004152719 | hypothetical protein | - |
| HB637_RS27670 (HB637_27680) | 55063..55383 | + | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HB637_RS27675 (HB637_27685) | 55373..55651 | + | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| HB637_RS27680 (HB637_27690) | 55652..56065 | + | 414 | WP_004152722 | helix-turn-helix domain-containing protein | - |
| HB637_RS27700 (HB637_27710) | 56899..57720 | + | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| HB637_RS27705 (HB637_27715) | 57753..58082 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| HB637_RS27710 (HB637_27720) | 58115..58600 | - | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| HB637_RS27715 (HB637_27725) | 59034..59426 | + | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HB637_RS27720 (HB637_27730) | 59640..60335 | + | 696 | WP_004178061 | transcriptional regulator TraJ family protein | - |
| HB637_RS27725 (HB637_27735) | 60419..60790 | + | 372 | WP_004208838 | TraY domain-containing protein | - |
| HB637_RS27730 (HB637_27740) | 60844..61212 | + | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| HB637_RS27735 (HB637_27745) | 61226..61531 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| HB637_RS27740 (HB637_27750) | 61551..62117 | + | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| HB637_RS27745 (HB637_27755) | 62104..62844 | + | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| HB637_RS27750 (HB637_27760) | 62844..64268 | + | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HB637_RS27755 (HB637_27765) | 64261..64857 | + | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| HB637_RS27760 (HB637_27770) | 64880..65449 | + | 570 | WP_004152598 | type IV conjugative transfer system lipoprotein TraV | traV |
| HB637_RS27765 (HB637_27775) | 65581..65991 | + | 411 | WP_004152597 | lipase chaperone | - |
| HB637_RS27770 (HB637_27780) | 65996..66286 | + | 291 | WP_004152596 | hypothetical protein | - |
| HB637_RS27775 (HB637_27785) | 66310..66528 | + | 219 | WP_004171484 | hypothetical protein | - |
| HB637_RS27780 (HB637_27790) | 66529..66846 | + | 318 | WP_004152595 | hypothetical protein | - |
| HB637_RS27785 (HB637_27795) | 66913..67317 | + | 405 | WP_004152594 | hypothetical protein | - |
| HB637_RS27790 (HB637_27800) | 67613..68011 | + | 399 | WP_004153071 | hypothetical protein | - |
| HB637_RS27795 (HB637_27805) | 68083..70722 | + | 2640 | WP_004152592 | type IV secretion system protein TraC | virb4 |
| HB637_RS27800 (HB637_27810) | 70722..71111 | + | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| HB637_RS27805 (HB637_27815) | 71111..71737 | + | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| HB637_RS27810 (HB637_27820) | 71781..72143 | + | 363 | Protein_81 | TraU family protein | - |
| HB637_RS27815 (HB637_27825) | 72207..72911 | + | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| HB637_RS27820 (HB637_27830) | 73167..73487 | + | 321 | WP_015632449 | hypothetical protein | - |
| HB637_RS27825 (HB637_27835) | 73758..74090 | - | 333 | WP_015632448 | hypothetical protein | - |
| HB637_RS27830 (HB637_27840) | 74486..74797 | + | 312 | WP_016162079 | plasmid mobilization protein MobA | - |
| HB637_RS27835 (HB637_27845) | 74800..76728 | + | 1929 | WP_015632446 | TraI/MobA(P) family conjugative relaxase | - |
Region 2: 82305..104146
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HB637_RS27850 (HB637_27860) | 77365..77772 | + | 408 | WP_015632445 | transposase | - |
| HB637_RS27855 (HB637_27865) | 77769..78118 | + | 350 | Protein_90 | IS66 family insertion sequence element accessory protein TnpB | - |
| HB637_RS27860 (HB637_27870) | 78148..79737 | + | 1590 | WP_015632444 | IS66 family transposase | - |
| HB637_RS27865 (HB637_27875) | 79760..80065 | - | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| HB637_RS27870 (HB637_27880) | 80111..82303 | - | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| HB637_RS27875 (HB637_27885) | 82305..83432 | - | 1128 | WP_157787237 | thioredoxin fold domain-containing protein | trbB |
| HB637_RS27880 (HB637_27890) | 83407..84825 | - | 1419 | WP_015632440 | hypothetical protein | trbA |
| HB637_RS27885 (HB637_27895) | 84927..85550 | - | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HB637_RS27890 (HB637_27900) | 85616..87760 | - | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| HB637_RS27895 (HB637_27905) | 87802..88383 | - | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| HB637_RS27900 (HB637_27910) | 88380..89576 | - | 1197 | WP_015632436 | conjugal transfer protein TraW | traW |
| HB637_RS27905 (HB637_27915) | 89587..92634 | - | 3048 | WP_015632435 | conjugal transfer protein | traU |
| HB637_RS27910 (HB637_27920) | 92644..92958 | - | 315 | WP_123677146 | hypothetical protein | traT |
| HB637_RS27915 (HB637_27925) | 93251..93652 | - | 402 | WP_015632433 | DUF6750 family protein | traR |
| HB637_RS27920 (HB637_27930) | 93706..94239 | - | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| HB637_RS27925 (HB637_27935) | 94249..94968 | - | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| HB637_RS27930 (HB637_27940) | 94974..96314 | - | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| HB637_RS27935 (HB637_27945) | 96318..97421 | - | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| HB637_RS27940 (HB637_27950) | 97432..98136 | - | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| HB637_RS27945 (HB637_27955) | 98172..98525 | - | 354 | WP_015632427 | hypothetical protein | traL |
| HB637_RS27950 (HB637_27960) | 98559..101882 | - | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| HB637_RS27955 (HB637_27965) | 101900..102166 | - | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| HB637_RS27960 (HB637_27970) | 102163..103350 | - | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| HB637_RS27965 (HB637_27975) | 103328..104146 | - | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| HB637_RS27970 (HB637_27980) | 104143..104610 | - | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| HB637_RS27975 (HB637_27985) | 104633..105112 | - | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| HB637_RS27980 (HB637_27990) | 105810..106121 | - | 312 | WP_015632421 | hypothetical protein | - |
| HB637_RS27985 (HB637_27995) | 106155..106358 | - | 204 | WP_032495743 | hypothetical protein | - |
| HB637_RS27990 (HB637_28000) | 106376..106909 | - | 534 | WP_015632420 | transcription termination/antitermination NusG family protein | - |
| HB637_RS27995 (HB637_28005) | 107177..107755 | + | 579 | WP_015632419 | hypothetical protein | - |
| HB637_RS28000 (HB637_28010) | 107737..108207 | + | 471 | WP_032441927 | hypothetical protein | - |
| HB637_RS28005 (HB637_28015) | 108207..108593 | + | 387 | WP_015632417 | acyl-CoA thioesterase | - |
Host bacterium
| ID | 5471 | GenBank | NZ_CP050373 |
| Plasmid name | p50595_IncFII | Incompatibility group | IncFIB |
| Plasmid size | 127925 bp | Coordinate of oriT [Strand] | 58673..58722 [-] |
| Host baterium | Klebsiella pneumoniae strain 50595 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | silP, silA, silB, silF, silC, silR, silS, silE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |