Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105028
Name   oriT_p47733_ARR2 in_silico
Organism   Klebsiella pneumoniae strain 47733
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP050361 (59223..59304 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p47733_ARR2
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3519 GenBank   WP_015632446
Name   Relaxase_HB636_RS27230_p47733_ARR2 insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   3481 GenBank   WP_015632442
Name   trbC_HB636_RS27265_p47733_ARR2 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 67165..89006

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB636_RS27245 (HB636_27260) 62225..62632 + 408 WP_015632445 transposase -
HB636_RS27250 (HB636_27265) 62629..62978 + 350 Protein_78 IS66 family insertion sequence element accessory protein TnpB -
HB636_RS27255 (HB636_27270) 63008..64597 + 1590 WP_015632444 IS66 family transposase -
HB636_RS27260 (HB636_27275) 64620..64925 - 306 WP_015632443 phospholipase D-like domain-containing protein -
HB636_RS27265 (HB636_27280) 64971..67163 - 2193 WP_015632442 F-type conjugative transfer protein TrbC -
HB636_RS27270 (HB636_27285) 67165..68292 - 1128 WP_157787237 thioredoxin fold domain-containing protein trbB
HB636_RS27275 (HB636_27290) 68267..69685 - 1419 WP_015632440 hypothetical protein trbA
HB636_RS27280 (HB636_27295) 69787..70410 - 624 WP_015632439 plasmid IncI1-type surface exclusion protein ExcA -
HB636_RS27285 (HB636_27300) 70476..72620 - 2145 WP_015632438 DotA/TraY family protein traY
HB636_RS27290 (HB636_27305) 72662..73243 - 582 WP_015632437 conjugal transfer protein TraX -
HB636_RS27295 (HB636_27310) 73240..74436 - 1197 WP_015632436 conjugal transfer protein TraW traW
HB636_RS27300 (HB636_27315) 74447..77494 - 3048 WP_015632435 conjugal transfer protein traU
HB636_RS27305 (HB636_27320) 77504..77818 - 315 WP_123677146 hypothetical protein traT
HB636_RS27310 (HB636_27325) 78111..78512 - 402 WP_015632433 DUF6750 family protein traR
HB636_RS27315 (HB636_27330) 78566..79099 - 534 WP_015632432 conjugal transfer protein TraQ traQ
HB636_RS27320 (HB636_27335) 79109..79828 - 720 WP_015632431 conjugal transfer protein TraP traP
HB636_RS27325 (HB636_27340) 79834..81174 - 1341 WP_015632430 conjugal transfer protein TraO traO
HB636_RS27330 (HB636_27345) 81178..82281 - 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
HB636_RS27335 (HB636_27350) 82292..82996 - 705 WP_015632428 DotI/IcmL/TraM family protein traM
HB636_RS27340 (HB636_27355) 83032..83385 - 354 WP_015632427 hypothetical protein traL
HB636_RS27345 (HB636_27360) 83419..86742 - 3324 WP_032495745 LPD7 domain-containing protein -
HB636_RS27350 (HB636_27365) 86760..87026 - 267 WP_032441926 IcmT/TraK family protein traK
HB636_RS27355 (HB636_27370) 87023..88210 - 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
HB636_RS27360 (HB636_27375) 88188..89006 - 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
HB636_RS27365 (HB636_27380) 89003..89470 - 468 WP_015632424 DotD/TraH family lipoprotein -
HB636_RS27370 (HB636_27385) 89493..89972 - 480 WP_015632423 lytic transglycosylase domain-containing protein -
HB636_RS27375 (HB636_27390) 90670..90981 - 312 WP_015632421 hypothetical protein -
HB636_RS27380 (HB636_27395) 91015..91218 - 204 WP_032495743 hypothetical protein -
HB636_RS27385 (HB636_27400) 91236..91703 - 468 WP_032495742 transcription termination/antitermination NusG family protein -
HB636_RS27390 (HB636_27405) 92037..92615 + 579 WP_015632419 hypothetical protein -
HB636_RS27395 (HB636_27410) 92597..93067 + 471 WP_032441927 hypothetical protein -
HB636_RS27400 (HB636_27415) 93067..93453 + 387 WP_015632417 acyl-CoA thioesterase -

Host bacterium

ID   5466 GenBank   NZ_CP050361
Plasmid name   p47733_ARR2 Incompatibility group   IncFII
Plasmid size   107451 bp Coordinate of oriT [Strand]   59223..59304 [+]
Host baterium   Klebsiella pneumoniae strain 47733

Cargo genes

Drug resistance gene   rmtF, ARR-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -