Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   105025
Name   oriT_pC2972-6 in_silico
Organism   Klebsiella pneumoniae strain C2972
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP039807 (11984..13690 [-], 1707 nt)
oriT length   1707 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1707 nt

>oriT_pC2972-6
GCCGTATGAATGACATTTATTTCCGTCCAATCCATCGCAATCAATATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTAATCATGTTGGCGGCAATAGTTACCGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCTGAGCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCCACGATACAATATGGACTCCAGCAGTATTTTACGCAGCCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCTAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATAAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGTAGAGTGAAAGGATAACTGACATATCCATTTTTATATGAGATGTTCGGAATGATAAATGAAAATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGTTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAATACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCCGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCTACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCAAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   3476 GenBank   WP_094309419
Name   t4cp2_FBF57_RS29200_pC2972-6 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65670.93 Da        Isoelectric Point: 7.4566

>WP_094309419.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAVRRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(174-280)

TraM recognition site of TraD and TraG (TraG-D_C)

(399-490)

  Protein structure



No available structure.



ID   3477 GenBank   WP_012457110
Name   t4cp2_FBF57_RS29260_pC2972-6 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103056.99 Da        Isoelectric Point: 7.4625

>WP_012457110.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10031..23580

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FBF57_RS29175 (FBF57_29315) 5877..6491 + 615 WP_014839983 recombinase family protein -
FBF57_RS29180 (FBF57_29320) 6810..7466 - 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -
FBF57_RS29185 (FBF57_29330) 7971..8675 - 705 WP_001067855 IS6-like element IS26 family transposase -
FBF57_RS29190 (FBF57_29335) 8740..8919 - 180 Protein_12 helix-turn-helix domain-containing protein -
FBF57_RS29195 (FBF57_29340) 9267..10022 - 756 WP_023316386 MobC family replication-relaxation protein -
FBF57_RS29200 (FBF57_29345) 10031..11767 - 1737 WP_094309419 type IV secretion system DNA-binding domain-containing protein virb4
FBF57_RS29205 (FBF57_29360) 12548..12853 - 306 WP_094309429 dpoa decarboxylase -
FBF57_RS29210 (FBF57_29365) 12887..13192 - 306 WP_012457118 hypothetical protein -
FBF57_RS29215 (FBF57_29370) 13228..13626 - 399 WP_162262155 cag pathogenicity island Cag12 family protein -
FBF57_RS29220 (FBF57_29375) 13623..14648 - 1026 WP_032442691 P-type DNA transfer ATPase VirB11 virB11
FBF57_RS29225 (FBF57_29380) 14638..15906 - 1269 WP_139968916 VirB10/TraB/TrbI family type IV secretion system protein virB10
FBF57_RS29230 (FBF57_29385) 15953..16861 - 909 WP_012457114 P-type conjugative transfer protein VirB9 virB9
FBF57_RS29235 (FBF57_29390) 16858..17541 - 684 WP_004178111 type IV secretion system protein virB8
FBF57_RS29430 17534..17671 - 138 WP_039109361 hypothetical protein -
FBF57_RS29245 (FBF57_29395) 17763..18839 - 1077 WP_012457112 type IV secretion system protein virB6
FBF57_RS29250 (FBF57_29400) 18851..19078 - 228 WP_004178109 EexN family lipoprotein -
FBF57_RS29255 (FBF57_29405) 19088..19798 - 711 WP_012457111 type IV secretion system protein -
FBF57_RS29260 (FBF57_29410) 19817..22555 - 2739 WP_012457110 VirB3 family type IV secretion system protein virb4
FBF57_RS29265 (FBF57_29415) 22568..22873 - 306 WP_012457109 TrbC/VirB2 family protein virB2
FBF57_RS29270 (FBF57_29420) 22870..23580 - 711 WP_004178175 lytic transglycosylase domain-containing protein virB1
FBF57_RS29770 23579..23701 + 123 WP_255093128 hypothetical protein -
FBF57_RS29275 (FBF57_29425) 23698..23916 - 219 WP_032722781 hypothetical protein -
FBF57_RS29280 (FBF57_29430) 23921..24136 - 216 WP_004178170 hypothetical protein -
FBF57_RS29285 (FBF57_29435) 24208..24663 - 456 WP_009309974 transcription termination/antitermination NusG family protein -
FBF57_RS29290 (FBF57_29440) 24917..25276 + 360 WP_012457108 helix-turn-helix transcriptional regulator -
FBF57_RS29295 (FBF57_29445) 25740..26441 + 702 WP_258549312 RepA family replication protein -
FBF57_RS29300 (FBF57_29450) 27306..27635 + 330 WP_000493286 type II toxin-antitoxin system RelE/ParE family toxin -
FBF57_RS29305 (FBF57_29455) 27616..27897 + 282 WP_000780222 helix-turn-helix transcriptional regulator -

Host bacterium

ID   5463 GenBank   NZ_CP039807
Plasmid name   pC2972-6 Incompatibility group   IncFII
Plasmid size   35466 bp Coordinate of oriT [Strand]   11984..13690 [-]
Host baterium   Klebsiella pneumoniae strain C2972

Cargo genes

Drug resistance gene   qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -