Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105023 |
| Name | oriT_pC2972-3  |
| Organism | Klebsiella pneumoniae strain C2972 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP039804 (1494..3361 [+], 1868 nt) |
| oriT length | 1868 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 1868 nt
>oriT_pC2972-3
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAAATAGGACAGAATATCTGTAGCCATGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCCTTTGACCCTGGGGATTTTGGCGCGGCGTTCAGCCACGACATTACCCCCATGCGGCAGGCAATAAGGGCGTCCAGCCCGCATGTACTGGCGCACGCCGTTTCGTGCAGGGAAGCATGCTTTCCTGCACATGGTCGCAACGCGGCCACCTTCTTCGCCCCAGACACTCGCCCCATATTTTCGCCCGACACCATGGGGCGAATTGATGGGGCGATGTCATGGGGCGAGTAGCTGACTAAGGAGGCATCATGATTTTAAATAAGC
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAAATAGGACAGAATATCTGTAGCCATGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCCTTTGACCCTGGGGATTTTGGCGCGGCGTTCAGCCACGACATTACCCCCATGCGGCAGGCAATAAGGGCGTCCAGCCCGCATGTACTGGCGCACGCCGTTTCGTGCAGGGAAGCATGCTTTCCTGCACATGGTCGCAACGCGGCCACCTTCTTCGCCCCAGACACTCGCCCCATATTTTCGCCCGACACCATGGGGCGAATTGATGGGGCGATGTCATGGGGCGAGTAGCTGACTAAGGAGGCATCATGATTTTAAATAAGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3473 | GenBank | WP_063116534 |
| Name | t4cp2_FBF57_RS27810_pC2972-3 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69595.85 Da Isoelectric Point: 6.2735
>WP_063116534.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 3474 | GenBank | WP_094965467 |
| Name | t4cp2_FBF57_RS28235_pC2972-3 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103112.07 Da Isoelectric Point: 7.4625
>WP_094965467.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacteriaceae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAVLFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAVLFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 107494..115110
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FBF57_RS28190 | 104028..104954 | + | 927 | WP_000039782 | hypothetical protein | - |
| FBF57_RS28195 (FBF57_28305) | 105094..105279 | + | 186 | WP_000205185 | AlpA family transcriptional regulator | - |
| FBF57_RS28200 (FBF57_28310) | 105301..105483 | + | 183 | WP_001513329 | hypothetical protein | - |
| FBF57_RS28205 (FBF57_28315) | 105468..106049 | + | 582 | WP_001593435 | DUF2857 domain-containing protein | - |
| FBF57_RS28210 (FBF57_28320) | 106039..106248 | + | 210 | WP_001112403 | TraR/DksA family transcriptional regulator | - |
| FBF57_RS28215 (FBF57_28325) | 106470..106619 | + | 150 | Protein_82 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| FBF57_RS28220 (FBF57_28330) | 107188..107421 | + | 234 | WP_001231171 | hypothetical protein | - |
| FBF57_RS28225 (FBF57_28335) | 107494..108204 | + | 711 | WP_001593436 | lytic transglycosylase domain-containing protein | virB1 |
| FBF57_RS28230 (FBF57_28340) | 108204..108497 | + | 294 | WP_000600171 | TrbC/VirB2 family protein | virB2 |
| FBF57_RS28235 (FBF57_28345) | 108510..111248 | + | 2739 | WP_094965467 | VirB3 family type IV secretion system protein | virb4 |
| FBF57_RS28240 (FBF57_28350) | 111266..111973 | + | 708 | WP_000857358 | type IV secretion system protein | - |
| FBF57_RS28245 (FBF57_28355) | 111981..112223 | + | 243 | WP_001593437 | EexN family lipoprotein | - |
| FBF57_RS28250 (FBF57_28360) | 112227..113300 | + | 1074 | WP_001593439 | type IV secretion system protein | virB6 |
| FBF57_RS28260 (FBF57_28365) | 113522..114205 | + | 684 | WP_000003229 | type IV secretion system protein | virB8 |
| FBF57_RS28265 (FBF57_28370) | 114202..115110 | + | 909 | WP_000976328 | P-type conjugative transfer protein VirB9 | virB9 |
Host bacterium
| ID | 5461 | GenBank | NZ_CP039804 |
| Plasmid name | pC2972-3 | Incompatibility group | - |
| Plasmid size | 115858 bp | Coordinate of oriT [Strand] | 1494..3361 [+] |
| Host baterium | Klebsiella pneumoniae strain C2972 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |