Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105022 |
| Name | oriT_pC2972-2  |
| Organism | Klebsiella pneumoniae strain C2972 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP039803 (1872..1921 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pC2972-2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3515 | GenBank | WP_162262139 |
| Name | traI_FBF57_RS26675_pC2972-2 |
UniProt ID | _ |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190399.86 Da Isoelectric Point: 6.0893
>WP_162262139.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTSDASVVRQAPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTQKQEKEIEK
ALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTSDASVVRQAPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTQKQEKEIEK
ALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3472 | GenBank | WP_014386205 |
| Name | traD_FBF57_RS26670_pC2972-2 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85924.97 Da Isoelectric Point: 5.1787
>WP_014386205.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAVAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAVAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1314..30993
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FBF57_RS26475 (FBF57_26525) | 98..919 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| FBF57_RS26480 (FBF57_26530) | 952..1281 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| FBF57_RS26485 (FBF57_26535) | 1314..1799 | - | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| FBF57_RS26490 (FBF57_26540) | 2190..2606 | + | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| FBF57_RS26495 (FBF57_26545) | 2799..3518 | + | 720 | WP_014386192 | conjugal transfer protein | - |
| FBF57_RS26500 (FBF57_26550) | 3651..3857 | + | 207 | WP_171773970 | TraY domain-containing protein | - |
| FBF57_RS26505 (FBF57_26555) | 3919..4287 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| FBF57_RS26510 (FBF57_26560) | 4301..4606 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| FBF57_RS26515 (FBF57_26565) | 4626..5192 | + | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| FBF57_RS26520 (FBF57_26570) | 5179..5919 | + | 741 | WP_014386193 | type-F conjugative transfer system secretin TraK | traK |
| FBF57_RS26525 (FBF57_26575) | 5919..7343 | + | 1425 | WP_162262137 | F-type conjugal transfer pilus assembly protein TraB | traB |
| FBF57_RS26530 (FBF57_26580) | 7336..7908 | + | 573 | WP_014386195 | conjugal transfer pilus-stabilizing protein TraP | - |
| FBF57_RS26535 (FBF57_26585) | 7955..8539 | + | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| FBF57_RS26540 (FBF57_26590) | 8670..9080 | + | 411 | WP_014386197 | hypothetical protein | - |
| FBF57_RS26545 (FBF57_26595) | 9085..9375 | + | 291 | WP_014386198 | hypothetical protein | - |
| FBF57_RS26550 (FBF57_26600) | 9399..9617 | + | 219 | WP_004195468 | hypothetical protein | - |
| FBF57_RS26555 (FBF57_26605) | 9618..9929 | + | 312 | WP_029497417 | hypothetical protein | - |
| FBF57_RS26560 (FBF57_26610) | 9996..10412 | + | 417 | WP_032408906 | hypothetical protein | - |
| FBF57_RS26570 (FBF57_26625) | 10777..11175 | + | 399 | WP_014343490 | hypothetical protein | - |
| FBF57_RS26575 (FBF57_26630) | 11247..13886 | + | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
| FBF57_RS26580 (FBF57_26635) | 13886..14275 | + | 390 | WP_013214025 | type-F conjugative transfer system protein TrbI | - |
| FBF57_RS26585 (FBF57_26640) | 14275..14910 | + | 636 | WP_162262138 | type-F conjugative transfer system protein TraW | traW |
| FBF57_RS26590 (FBF57_26645) | 14945..15346 | + | 402 | WP_004194979 | hypothetical protein | - |
| FBF57_RS26595 (FBF57_26650) | 15343..16332 | + | 990 | WP_029497420 | conjugal transfer pilus assembly protein TraU | traU |
| FBF57_RS26600 (FBF57_26655) | 16345..16983 | + | 639 | WP_004193871 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| FBF57_RS26605 (FBF57_26660) | 17042..18997 | + | 1956 | WP_014343489 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| FBF57_RS26610 (FBF57_26665) | 19029..19283 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| FBF57_RS26615 (FBF57_26670) | 19261..19509 | + | 249 | WP_013214029 | hypothetical protein | - |
| FBF57_RS26620 (FBF57_26675) | 19522..19848 | + | 327 | WP_013214030 | hypothetical protein | - |
| FBF57_RS26625 (FBF57_26680) | 19869..20621 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| FBF57_RS26630 (FBF57_26685) | 20632..20871 | + | 240 | WP_032440623 | type-F conjugative transfer system pilin chaperone TraQ | - |
| FBF57_RS26635 (FBF57_26690) | 20843..21400 | + | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| FBF57_RS26640 (FBF57_26695) | 21446..21889 | + | 444 | WP_013214032 | F-type conjugal transfer protein TrbF | - |
| FBF57_RS26645 (FBF57_26700) | 21867..23246 | + | 1380 | WP_072157884 | conjugal transfer pilus assembly protein TraH | traH |
| FBF57_RS26650 (FBF57_26705) | 23246..26068 | + | 2823 | WP_013214034 | conjugal transfer mating-pair stabilization protein TraG | traG |
| FBF57_RS26655 (FBF57_26710) | 26091..26693 | + | 603 | WP_013214035 | conjugal transfer protein TraS | - |
| FBF57_RS26660 (FBF57_26715) | 26944..27675 | + | 732 | WP_013214036 | conjugal transfer complement resistance protein TraT | - |
| FBF57_RS26665 | 27868..28557 | + | 690 | WP_014343485 | hypothetical protein | - |
| FBF57_RS26670 (FBF57_26725) | 28684..30993 | + | 2310 | WP_014386205 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 5460 | GenBank | NZ_CP039803 |
| Plasmid name | pC2972-2 | Incompatibility group | IncFII |
| Plasmid size | 214331 bp | Coordinate of oriT [Strand] | 1872..1921 [-] |
| Host baterium | Klebsiella pneumoniae strain C2972 |
Cargo genes
| Drug resistance gene | blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | arsR, arsD, arsA, arsB, arsC, arsH, pcoE, pcoS, pcoR, pcoD, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |