Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 105021 |
| Name | oriT_pN069_1  |
| Organism | Enterobacter hormaechei subsp. hoffmannii strain OIPH-N069 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP019818 (43137..43287 [+], 151 nt) |
| oriT length | 151 nt |
| IRs (inverted repeats) | 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 151 nt
>oriT_pN069_1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3514 | GenBank | WP_151819900 |
| Name | mobH_G7Y81_RS22565_pN069_1 |
UniProt ID | _ |
| Length | 992 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 992 a.a. Molecular weight: 109596.82 Da Isoelectric Point: 4.8526
>WP_151819900.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEEPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEEPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3471 | GenBank | WP_000637386 |
| Name | traC_G7Y81_RS22650_pN069_1 |
UniProt ID | _ |
| Length | 815 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 815 a.a. Molecular weight: 92590.87 Da Isoelectric Point: 5.9222
>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43638..66374
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G7Y81_RS24295 | 41389..41469 | + | 81 | Protein_59 | hypothetical protein | - |
| G7Y81_RS24300 | 41776..43086 | + | 1311 | Protein_60 | conjugative transfer system coupling protein TraD | - |
| G7Y81_RS22575 (OIPHN069_44230) | 43136..43681 | + | 546 | WP_227549712 | hypothetical protein | - |
| G7Y81_RS22580 (OIPHN069_44240) | 43638..44267 | + | 630 | WP_000743450 | DUF4400 domain-containing protein | tfc7 |
| G7Y81_RS22585 | 44277..44723 | + | 447 | WP_151819903 | hypothetical protein | - |
| G7Y81_RS22590 (OIPHN069_44260) | 44733..45110 | + | 378 | WP_151819904 | hypothetical protein | - |
| G7Y81_RS22595 (OIPHN069_44270) | 45110..45772 | + | 663 | WP_151819905 | hypothetical protein | - |
| G7Y81_RS22600 | 45952..46095 | + | 144 | WP_164457053 | hypothetical protein | - |
| G7Y81_RS22605 (OIPHN069_44280) | 46109..46474 | + | 366 | WP_227549713 | hypothetical protein | - |
| G7Y81_RS22610 (OIPHN069_44290) | 46619..46900 | + | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| G7Y81_RS22615 (OIPHN069_44300) | 46897..47523 | + | 627 | WP_001049716 | TraE/TraK family type IV conjugative transfer system protein | traE |
| G7Y81_RS22620 (OIPHN069_44310) | 47507..48424 | + | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| G7Y81_RS22625 (OIPHN069_44320) | 48424..49737 | + | 1314 | WP_164597232 | TraB/VirB10 family protein | traB |
| G7Y81_RS22630 (OIPHN069_44330) | 49734..50312 | + | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| G7Y81_RS22635 (OIPHN069_44340) | 50325..50708 | + | 384 | WP_227549714 | TraA family conjugative transfer protein | - |
| G7Y81_RS22640 (OIPHN069_44350) | 50909..56395 | + | 5487 | WP_151819907 | Ig-like domain-containing protein | - |
| G7Y81_RS22645 (OIPHN069_44360) | 56544..57251 | + | 708 | WP_151819908 | DsbC family protein | - |
| G7Y81_RS22650 (OIPHN069_44370) | 57248..59695 | + | 2448 | WP_000637386 | type IV secretion system protein TraC | virb4 |
| G7Y81_RS22655 (OIPHN069_44380) | 59710..60027 | + | 318 | WP_000351984 | hypothetical protein | - |
| G7Y81_RS22660 (OIPHN069_44390) | 60024..60554 | + | 531 | WP_001010738 | S26 family signal peptidase | - |
| G7Y81_RS22665 (OIPHN069_44400) | 60517..61782 | + | 1266 | WP_164597187 | TrbC family F-type conjugative pilus assembly protein | traW |
| G7Y81_RS22670 (OIPHN069_44410) | 61779..62450 | + | 672 | WP_121926691 | EAL domain-containing protein | - |
| G7Y81_RS22675 | 62471..63454 | + | 984 | WP_232070533 | TraU family protein | traU |
| G7Y81_RS22680 (OIPHN069_44430) | 63570..66374 | + | 2805 | WP_232070532 | conjugal transfer mating pair stabilization protein TraN | traN |
| G7Y81_RS22685 (OIPHN069_44440) | 66715..67575 | - | 861 | WP_164597211 | hypothetical protein | - |
| G7Y81_RS22690 (OIPHN069_44450) | 68352..68903 | - | 552 | WP_123014137 | sugar O-acetyltransferase | - |
| G7Y81_RS22695 (OIPHN069_44460) | 69016..69414 | - | 399 | WP_164597213 | FosG family fosfomycin resistance glutathione transferase | - |
| G7Y81_RS22700 (OIPHN069_44470) | 69905..70303 | - | 399 | WP_011787738 | hypothetical protein | - |
Host bacterium
| ID | 5459 | GenBank | NZ_AP019818 |
| Plasmid name | pN069_1 | Incompatibility group | IncA/C2 |
| Plasmid size | 136816 bp | Coordinate of oriT [Strand] | 43137..43287 [+] |
| Host baterium | Enterobacter hormaechei subsp. hoffmannii strain OIPH-N069 |
Cargo genes
| Drug resistance gene | fosG, blaCTX-M-2, aadA2, aac(6')-Iae, qacE, sul1, blaKHM-1 |
| Virulence gene | - |
| Metal resistance gene | merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |