Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104970
Name   oriT_pPS00120.1A.1 in_silico
Organism   Staphylococcus aureus strain 199
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP031668 (12887..13068 [-], 182 nt)
oriT length   182 nt
IRs (inverted repeats)      156..161, 171..176  (ATTTTA..TAAAAT)
 88..95, 99..106  (TATCTGGC..GCCAGATA)
 44..51, 64..71  (GTCTTTTT..AAAAAGAC)
 22..28, 33..39  (TGTCACA..TGTGACA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 182 nt

>oriT_pPS00120.1A.1
TGTGACAAACTCAATATATTGTGTCACAAAAGTGTGACATTTCGTCTTTTTATGCCCCCATGCAAAAAGACCGACGAAGTCTTGAAATATCTGGCTTTGCCAGATACCTCATCTATAGATGGGGTCATATTTCCCTTATGCTCTTACTTACGGAGATTTTAGAGTTAAATTAAAATTTCTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3492 GenBank   WP_000277654
Name   Rep_trans_DD546_RS15115_pPS00120.1A.1 insolico UniProt ID   A0A0H2WVC8
Length   314 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 314 a.a.        Molecular weight: 37642.61 Da        Isoelectric Point: 7.2208

>WP_000277654.1 MULTISPECIES: replication initiation factor domain-containing protein [Bacteria]
MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSFDAMTIVGNLNRDNAQALSKFMSVEP
QIRLWDILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTR
LDLAFDFEDDLSDYYAMSDKAVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHL
WRVEIELKRDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEIS
PVDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK

  Protein domains


Predicted by InterproScan.

Replication initiation factor (Rep_trans)

(138-285)


  Protein structure


Source ID Structure
AlphaFold DB A0A0H2WVC8


Host bacterium

ID   5408 GenBank   NZ_CP031668
Plasmid name   pPS00120.1A.1 Incompatibility group   -
Plasmid size   18320 bp Coordinate of oriT [Strand]   12887..13068 [-]
Host baterium   Staphylococcus aureus strain 199

Cargo genes

Drug resistance gene   tet(K)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21