Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104963 |
| Name | oriT_pML9ba2  |
| Organism | Klebsiella variicola subsp. variicola strain ML.9ba2 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP092633 (93011..93060 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 9..14, 17..22 (AAAATT..AATTTT) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pML9ba2
AAATCTGAAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGAAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3488 | GenBank | WP_240289798 |
| Name | traI_MKK01_RS26745_pML9ba2 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190642.70 Da Isoelectric Point: 6.1727
>WP_240289798.1 conjugative transfer relaxase/helicase TraI [Klebsiella variicola]
MMSIGSVKSAGSTGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGVNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIESVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAITGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKESISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRAHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPGETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLMFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEGARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSTGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGVNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIESVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAITGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKESISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRAHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPGETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLMFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEGARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3437 | GenBank | WP_240289799 |
| Name | traD_MKK01_RS26750_pML9ba2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86037.13 Da Isoelectric Point: 5.0045
>WP_240289799.1 type IV conjugative transfer system coupling protein TraD [Klebsiella variicola]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRRLDTQVDARLSALLQAREAEGSLARALFMPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPPILRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRRLDTQVDARLSALLQAREAEGSLARALFMPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPPILRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65022..90624
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKK01_RS26750 (MKK01_26750) | 65022..67334 | - | 2313 | WP_240289799 | type IV conjugative transfer system coupling protein TraD | virb4 |
| MKK01_RS26755 (MKK01_26755) | 67463..68152 | - | 690 | WP_240289800 | hypothetical protein | - |
| MKK01_RS26760 (MKK01_26760) | 68344..69075 | - | 732 | WP_032329926 | conjugal transfer complement resistance protein TraT | - |
| MKK01_RS26765 (MKK01_26765) | 69297..69821 | - | 525 | WP_117146741 | hypothetical protein | - |
| MKK01_RS26770 (MKK01_26770) | 69832..72675 | - | 2844 | WP_240289801 | conjugal transfer mating-pair stabilization protein TraG | traG |
| MKK01_RS26775 (MKK01_26775) | 72675..74054 | - | 1380 | WP_072198238 | conjugal transfer pilus assembly protein TraH | traH |
| MKK01_RS26780 (MKK01_26780) | 74032..74475 | - | 444 | WP_023158001 | F-type conjugal transfer protein TrbF | - |
| MKK01_RS26785 (MKK01_26785) | 74521..75078 | - | 558 | WP_117146735 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| MKK01_RS26790 (MKK01_26790) | 75050..75289 | - | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
| MKK01_RS26795 (MKK01_26795) | 75300..76052 | - | 753 | WP_049119076 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| MKK01_RS26800 (MKK01_26800) | 76073..76399 | - | 327 | WP_064144773 | hypothetical protein | - |
| MKK01_RS26805 (MKK01_26805) | 76410..76637 | - | 228 | WP_065808180 | conjugal transfer protein TrbE | - |
| MKK01_RS26810 (MKK01_26810) | 76627..76908 | - | 282 | WP_117146729 | hypothetical protein | - |
| MKK01_RS26815 (MKK01_26815) | 76941..78905 | - | 1965 | WP_240289802 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| MKK01_RS26820 (MKK01_26820) | 78964..79602 | - | 639 | WP_240289803 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| MKK01_RS26825 (MKK01_26825) | 79615..80574 | - | 960 | WP_168712236 | conjugal transfer pilus assembly protein TraU | traU |
| MKK01_RS26830 (MKK01_26830) | 80618..81244 | - | 627 | WP_004163052 | type-F conjugative transfer system protein TraW | traW |
| MKK01_RS26835 (MKK01_26835) | 81244..81633 | - | 390 | WP_020326931 | type-F conjugative transfer system protein TrbI | - |
| MKK01_RS26840 (MKK01_26840) | 81633..84272 | - | 2640 | WP_240289804 | type IV secretion system protein TraC | virb4 |
| MKK01_RS26845 (MKK01_26845) | 84344..84742 | - | 399 | WP_240289748 | hypothetical protein | - |
| MKK01_RS26850 (MKK01_26850) | 84750..85040 | - | 291 | WP_240289749 | hypothetical protein | - |
| MKK01_RS26855 (MKK01_26855) | 85037..85441 | - | 405 | WP_226614932 | hypothetical protein | - |
| MKK01_RS26860 (MKK01_26860) | 85508..85825 | - | 318 | WP_110244269 | hypothetical protein | - |
| MKK01_RS26865 (MKK01_26865) | 85826..86044 | - | 219 | WP_110244268 | hypothetical protein | - |
| MKK01_RS26870 (MKK01_26870) | 86068..86358 | - | 291 | WP_110244267 | hypothetical protein | - |
| MKK01_RS26875 (MKK01_26875) | 86387..86785 | - | 399 | WP_226614937 | hypothetical protein | - |
| MKK01_RS26880 (MKK01_26880) | 86940..87509 | - | 570 | WP_117146720 | type IV conjugative transfer system lipoprotein TraV | traV |
| MKK01_RS26885 (MKK01_26885) | 87582..89006 | - | 1425 | WP_240289750 | F-type conjugal transfer pilus assembly protein TraB | traB |
| MKK01_RS26890 (MKK01_26890) | 89006..89746 | - | 741 | WP_240289751 | type-F conjugative transfer system secretin TraK | traK |
| MKK01_RS26895 (MKK01_26895) | 89733..90299 | - | 567 | WP_240289752 | type IV conjugative transfer system protein TraE | traE |
| MKK01_RS26900 (MKK01_26900) | 90319..90624 | - | 306 | WP_110244263 | type IV conjugative transfer system protein TraL | traL |
| MKK01_RS26905 (MKK01_26905) | 90638..91006 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| MKK01_RS26910 (MKK01_26910) | 91068..91274 | - | 207 | WP_181403965 | TraY domain-containing protein | - |
| MKK01_RS26915 (MKK01_26915) | 91407..92126 | - | 720 | WP_165454125 | conjugal transfer protein TrbJ | - |
| MKK01_RS26920 (MKK01_26920) | 92326..92742 | - | 417 | WP_181403962 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| MKK01_RS26925 (MKK01_26925) | 93205..93612 | + | 408 | WP_223170089 | transglycosylase SLT domain-containing protein | - |
| MKK01_RS26930 (MKK01_26930) | 93650..94180 | - | 531 | WP_023292160 | antirestriction protein | - |
Host bacterium
| ID | 5401 | GenBank | NZ_CP092633 |
| Plasmid name | pML9ba2 | Incompatibility group | IncFIB |
| Plasmid size | 166327 bp | Coordinate of oriT [Strand] | 93011..93060 [+] |
| Host baterium | Klebsiella variicola subsp. variicola strain ML.9ba2 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | fecD, fecE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |