Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104961 |
| Name | oriT_pCR1B  |
| Organism | Enterococcus gilvus strain CR1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP030934 (60174..60203 [-], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_pCR1B
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3486 | GenBank | WP_114526088 |
| Name | mobQ_EGCR1_RS18685_pCR1B |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77455.52 Da Isoelectric Point: 9.2636
>WP_114526088.1 MobQ family relaxase [Enterococcus gilvus]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDRQTL
WNKMELAEKSPNAQLCRELNIALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDRQTL
WNKMELAEKSPNAQLCRELNIALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3434 | GenBank | WP_114526093 |
| Name | t4cp2_EGCR1_RS18730_pCR1B |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63105.17 Da Isoelectric Point: 4.6997
>WP_114526093.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus gilvus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKLEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQENDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSNFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKLEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQENDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSNFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 62885..72906
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGCR1_RS18660 (EGCR1_18665) | 58262..58579 | + | 318 | WP_002338433 | hypothetical protein | - |
| EGCR1_RS18665 (EGCR1_18670) | 58599..59096 | + | 498 | WP_114526086 | DnaJ domain-containing protein | - |
| EGCR1_RS18670 (EGCR1_18675) | 59130..59435 | + | 306 | WP_114526087 | hypothetical protein | - |
| EGCR1_RS18675 (EGCR1_18680) | 59458..59715 | - | 258 | WP_002325623 | hypothetical protein | - |
| EGCR1_RS18680 (EGCR1_18685) | 59718..60017 | - | 300 | WP_002325624 | hypothetical protein | - |
| EGCR1_RS18685 (EGCR1_18690) | 60309..62294 | + | 1986 | WP_114526088 | MobQ family relaxase | - |
| EGCR1_RS18690 (EGCR1_18695) | 62318..62650 | + | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| EGCR1_RS18695 (EGCR1_18700) | 62885..63268 | + | 384 | WP_001083514 | glycosyl transferase | trsC |
| EGCR1_RS18700 (EGCR1_18705) | 63285..63914 | + | 630 | WP_021109237 | hypothetical protein | trsD |
| EGCR1_RS18705 (EGCR1_18710) | 63925..65886 | + | 1962 | WP_114526089 | TrsE protein | virb4 |
| EGCR1_RS18710 (EGCR1_18715) | 65900..67252 | + | 1353 | WP_114526090 | conjugal transfer protein TraF | trsF |
| EGCR1_RS18715 (EGCR1_18720) | 67274..68383 | + | 1110 | WP_114526091 | lysozyme family protein | orf14 |
| EGCR1_RS18720 (EGCR1_18725) | 68396..68947 | + | 552 | WP_114526092 | hypothetical protein | - |
| EGCR1_RS18725 (EGCR1_18730) | 68952..69383 | + | 432 | WP_001085135 | hypothetical protein | - |
| EGCR1_RS18730 (EGCR1_18735) | 69376..71031 | + | 1656 | WP_114526093 | VirD4-like conjugal transfer protein, CD1115 family | - |
| EGCR1_RS18735 (EGCR1_18740) | 71049..71972 | + | 924 | WP_114526094 | hypothetical protein | - |
| EGCR1_RS18740 (EGCR1_18745) | 71974..72906 | + | 933 | WP_029486174 | conjugal transfer protein TrbL family protein | trsL |
| EGCR1_RS18745 (EGCR1_18750) | 72923..73891 | + | 969 | WP_114526095 | hypothetical protein | - |
| EGCR1_RS18750 (EGCR1_18755) | 73920..74273 | + | 354 | WP_002339801 | hypothetical protein | - |
| EGCR1_RS18755 (EGCR1_18760) | 74333..74710 | - | 378 | WP_114526096 | hypothetical protein | - |
| EGCR1_RS18760 (EGCR1_18765) | 74957..75202 | + | 246 | WP_047927842 | hypothetical protein | - |
| EGCR1_RS18765 (EGCR1_18770) | 75696..77243 | + | 1548 | WP_114526097 | type I restriction-modification system subunit M | - |
Host bacterium
| ID | 5399 | GenBank | NZ_CP030934 |
| Plasmid name | pCR1B | Incompatibility group | - |
| Plasmid size | 80244 bp | Coordinate of oriT [Strand] | 60174..60203 [-] |
| Host baterium | Enterococcus gilvus strain CR1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |