Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104959
Name   oriT_pSA20025921.2 in_silico
Organism   Salmonella enterica strain SA20025921
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP030216 (26983..27070 [+], 88 nt)
oriT length   88 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 88 nt

>oriT_pSA20025921.2
GGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3485 GenBank   WP_023233611
Name   nikB_CHC41_RS26200_pSA20025921.2 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103945.58 Da        Isoelectric Point: 7.4287

>WP_023233611.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRSEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLNGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYSDYIAANSNDEITLPSLELEKQWEPPKPM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   1552 GenBank   WP_023233610
Name   WP_023233610_pSA20025921.2 insolico UniProt ID   A0A3V7DIS6
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12675.68 Da        Isoelectric Point: 10.7699

>WP_023233610.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFTPVEDETIRKKAEYSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A3V7DIS6


T4CP

ID   3433 GenBank   WP_240197824
Name   t4cp2_CHC41_RS26185_pSA20025921.2 insolico UniProt ID   _
Length   530 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 530 a.a.        Molecular weight: 60110.07 Da        Isoelectric Point: 6.3050

>WP_240197824.1 conjugal transfer protein TrbC, partial [Salmonella enterica]
FTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGLDDQ
WPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIR
DSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLD
EVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTARTLKS
AAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDD
EKSCSSDPVVINRYISVDAPHLNRLHRLVPRTAQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVD
TFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVR
TERVGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1..20173

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHC41_RS26060 (CHC41_26055) 1..528 + 528 WP_114050680 conjugal transfer protein TraQ traQ
CHC41_RS26065 (CHC41_26060) 579..983 + 405 WP_000086959 IncI1-type conjugal transfer protein TraR traR
CHC41_RS26070 (CHC41_26065) 1047..1235 + 189 WP_114050681 putative conjugal transfer protein TraS -
CHC41_RS26075 (CHC41_26070) 1219..2019 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
CHC41_RS26080 (CHC41_26075) 2109..5153 + 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
CHC41_RS26085 (CHC41_26080) 5153..5767 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
CHC41_RS26090 (CHC41_26085) 5734..6936 + 1203 WP_114050682 IncI1-type conjugal transfer protein TraW traW
CHC41_RS26095 (CHC41_26090) 6965..7549 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
CHC41_RS26100 (CHC41_26095) 7646..9799 + 2154 Protein_9 IncI1-type conjugal transfer membrane protein TraY -
CHC41_RS26105 (CHC41_26100) 9880..10494 + 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
CHC41_RS26110 (CHC41_26105) 10592..10801 + 210 WP_001140545 hemolysin expression modulator Hha -
CHC41_RS27050 11010..11186 + 177 WP_001054900 hypothetical protein -
CHC41_RS26125 (CHC41_26120) 12110..12361 + 252 WP_001291965 hypothetical protein -
CHC41_RS26130 (CHC41_26125) 12433..12585 - 153 WP_001303307 Hok/Gef family protein -
CHC41_RS26135 (CHC41_26130) 13030..13239 + 210 WP_001140545 hemolysin expression modulator Hha -
CHC41_RS27055 13448..13624 + 177 WP_001054900 hypothetical protein -
CHC41_RS26150 (CHC41_26145) 14548..14799 + 252 WP_001291965 hypothetical protein -
CHC41_RS26155 (CHC41_26150) 14871..15023 - 153 WP_001303307 Hok/Gef family protein -
CHC41_RS26160 (CHC41_26155) 15315..16523 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
CHC41_RS26165 (CHC41_26160) 16542..17612 + 1071 WP_114050683 IncI1-type conjugal transfer protein TrbB trbB
CHC41_RS26170 (CHC41_26165) 17605..18967 + 1363 Protein_21 conjugal transfer protein TrbC -
CHC41_RS26180 (CHC41_26175) 19103..20173 + 1071 WP_023233614 IncI1-type conjugal transfer protein TrbB trbB
CHC41_RS27370 20166..20783 + 618 Protein_23 conjugal transfer protein TrbC -
CHC41_RS26185 (CHC41_26180) 20864..22457 + 1594 WP_240197824 conjugal transfer protein TrbC -
CHC41_RS26190 (CHC41_26185) 22645..23199 - 555 WP_023233612 GNAT family N-acetyltransferase -
CHC41_RS26195 (CHC41_26190) 23183..23506 - 324 WP_001419663 DUF1778 domain-containing protein -

Region 2: 81304..103717

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHC41_RS26585 (CHC41_26580) 76366..77433 + 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -
CHC41_RS26590 (CHC41_26585) 77433..77870 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
CHC41_RS26595 (CHC41_26590) 77884..79566 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
CHC41_RS26600 (CHC41_26595) 79559..80854 + 1296 WP_000752772 type 4b pilus protein PilO2 -
CHC41_RS26605 (CHC41_26600) 80841..81293 + 453 WP_001247334 type IV pilus biogenesis protein PilP -
CHC41_RS26610 (CHC41_26605) 81304..82857 + 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
CHC41_RS26615 (CHC41_26610) 82870..83967 + 1098 WP_001208805 type II secretion system F family protein -
CHC41_RS26620 (CHC41_26615) 83985..84599 + 615 WP_000959786 type 4 pilus major pilin -
CHC41_RS26625 (CHC41_26620) 84609..85169 + 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
CHC41_RS26630 (CHC41_26625) 85154..85810 + 657 WP_001193553 prepilin peptidase -
CHC41_RS26635 (CHC41_26630) 85810..87231 + 1422 WP_114050687 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
CHC41_RS27515 (CHC41_26655) 88479..88794 - 316 Protein_102 shufflon protein C -
CHC41_RS26665 (CHC41_26660) 88793..89947 + 1155 WP_001139958 site-specific integrase -
CHC41_RS26670 (CHC41_26665) 90098..90922 + 825 WP_114050688 conjugal transfer protein TraE traE
CHC41_RS26675 (CHC41_26670) 91008..92210 + 1203 WP_114050689 conjugal transfer protein TraF -
CHC41_RS26680 (CHC41_26675) 92270..92854 + 585 WP_114050690 histidine phosphatase family protein -
CHC41_RS26685 (CHC41_26680) 93249..93707 + 459 WP_114050691 IncI1-type conjugal transfer lipoprotein TraH -
CHC41_RS26690 (CHC41_26685) 93704..94522 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
CHC41_RS26695 (CHC41_26690) 94519..95667 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
CHC41_RS26700 (CHC41_26695) 95664..95954 + 291 WP_001372180 hypothetical protein traK
CHC41_RS26705 (CHC41_26700) 95969..96520 + 552 WP_000014584 phospholipase D family protein -
CHC41_RS26710 (CHC41_26705) 96610..100377 + 3768 WP_114050692 LPD7 domain-containing protein -
CHC41_RS26715 (CHC41_26710) 100395..100742 + 348 WP_001055900 conjugal transfer protein traL
CHC41_RS26720 (CHC41_26715) 100739..101431 + 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
CHC41_RS26725 (CHC41_26720) 101442..102425 + 984 WP_114050693 IncI1-type conjugal transfer protein TraN traN
CHC41_RS26730 (CHC41_26725) 102428..103717 + 1290 WP_001272000 conjugal transfer protein TraO traO

Host bacterium

ID   5397 GenBank   NZ_CP030216
Plasmid name   pSA20025921.2 Incompatibility group   IncI1
Plasmid size   104420 bp Coordinate of oriT [Strand]   26983..27070 [+]
Host baterium   Salmonella enterica strain SA20025921

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -