Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104951
Name   oriT_pSA20030575.1 in_silico
Organism   Salmonella enterica strain SA20030575
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP030182 (151799..152083 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pSA20030575.1
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3480 GenBank   WP_001011149
Name   mobH_CHC58_RS25265_pSA20030575.1 insolico UniProt ID   A0A0G3BA46
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113023.00 Da        Isoelectric Point: 4.5108

>WP_001011149.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure


Source ID Structure
AlphaFold DB A0A0G3BA46


T4CP

ID   3426 GenBank   WP_000167418
Name   traD_CHC58_RS25270_pSA20030575.1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 211520..220688

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHC58_RS25610 (CHC58_25600) 207057..207257 - 201 WP_000357614 hypothetical protein -
CHC58_RS25615 (CHC58_25605) 207275..208351 - 1077 WP_001015069 hypothetical protein -
CHC58_RS25625 (CHC58_25615) 208886..209761 + 876 WP_001282731 RepB family plasmid replication initiator protein -
CHC58_RS26415 210208..210552 - 345 WP_153253765 hypothetical protein -
CHC58_RS25635 (CHC58_25625) 211115..211468 + 354 WP_000424120 pili assembly chaperone -
CHC58_RS25640 (CHC58_25630) 211520..211837 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
CHC58_RS25645 (CHC58_25635) 211849..212634 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
CHC58_RS25650 (CHC58_25640) 212637..213869 + 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
CHC58_RS25655 (CHC58_25645) 213871..214311 + 441 WP_001224094 plasmid transfer protein HtdO -
CHC58_RS25660 (CHC58_25650) 214301..215659 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
CHC58_RS25665 (CHC58_25655) 215668..216180 + 513 WP_000429933 hypothetical protein -
CHC58_RS25670 (CHC58_25660) 216286..217038 + 753 WP_010892304 protein-disulfide isomerase HtdT -
CHC58_RS25675 (CHC58_25665) 217048..217998 + 951 WP_001022266 TraV family lipoprotein traV
CHC58_RS25680 (CHC58_25670) 218007..220688 + 2682 WP_001255537 IncHI-type conjugal transfer ATPase TrhC virb4
CHC58_RS25690 (CHC58_25680) 221318..221623 + 306 WP_162803767 hypothetical protein -
CHC58_RS26420 222301..222474 + 174 WP_153253763 hypothetical protein -
CHC58_RS26425 222505..222738 + 234 WP_162803770 hypothetical protein -

Host bacterium

ID   5389 GenBank   NZ_CP030182
Plasmid name   pSA20030575.1 Incompatibility group   IncHI1B
Plasmid size   224430 bp Coordinate of oriT [Strand]   151799..152083 [-]
Host baterium   Salmonella enterica strain SA20030575

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   pcoE, pcoS, pcoR, pcoD, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -