Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104949
Name   oriT_pSA20043041.1 in_silico
Organism   Salmonella enterica strain SA20043041
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP030232 (35551..35639 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSA20043041.1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3478 GenBank   WP_001299212
Name   nikB_CHD10_RS23620_pSA20043041.1 insolico UniProt ID   A0A5T4AK12
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103956.40 Da        Isoelectric Point: 7.2642

>WP_001299212.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWINFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A5T4AK12


Auxiliary protein

ID   1546 GenBank   WP_001283947
Name   WP_001283947_pSA20043041.1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   3424 GenBank   WP_001289276
Name   trbC_CHD10_RS23625_pSA20043041.1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41017..77156

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHD10_RS23625 (CHD10_23625) 38733..41024 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
CHD10_RS23630 (CHD10_23630) 41017..42087 - 1071 WP_000151577 IncI1-type conjugal transfer protein TrbB trbB
CHD10_RS23635 (CHD10_23635) 42106..43314 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
CHD10_RS24300 43605..43898 - 294 WP_240195195 hypothetical protein -
CHD10_RS24340 45112..45207 + 96 WP_001303310 DinQ-like type I toxin DqlB -
CHD10_RS24055 45272..45448 - 177 WP_001054904 hypothetical protein -
CHD10_RS23645 (CHD10_23645) 45840..46049 + 210 WP_000062603 HEAT repeat domain-containing protein -
CHD10_RS23650 (CHD10_23650) 46121..46771 - 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
CHD10_RS23655 (CHD10_23655) 46845..49013 - 2169 WP_000698357 DotA/TraY family protein traY
CHD10_RS23660 (CHD10_23660) 49110..49694 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
CHD10_RS23665 (CHD10_23665) 49723..50925 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
CHD10_RS23670 (CHD10_23670) 50892..51506 - 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
CHD10_RS23675 (CHD10_23675) 51506..54550 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
CHD10_RS23680 (CHD10_23680) 54640..55440 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
CHD10_RS23685 (CHD10_23685) 55424..55612 - 189 WP_001277255 putative conjugal transfer protein TraS -
CHD10_RS23690 (CHD10_23690) 55676..56080 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
CHD10_RS23695 (CHD10_23695) 56131..56658 - 528 WP_001055569 conjugal transfer protein TraQ traQ
CHD10_RS23700 (CHD10_23700) 56658..57362 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
CHD10_RS23705 (CHD10_23705) 57362..58651 - 1290 WP_001271997 conjugal transfer protein TraO traO
CHD10_RS23710 (CHD10_23710) 58654..59637 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
CHD10_RS23715 (CHD10_23715) 59648..60340 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
CHD10_RS23720 (CHD10_23720) 60337..60684 - 348 WP_001055900 conjugal transfer protein traL
CHD10_RS23725 (CHD10_23725) 60702..64469 - 3768 WP_001141542 LPD7 domain-containing protein -
CHD10_RS23730 (CHD10_23730) 64559..65110 - 552 WP_000014584 phospholipase D family protein -
CHD10_RS23735 (CHD10_23735) 65125..65415 - 291 WP_001299214 hypothetical protein traK
CHD10_RS23740 (CHD10_23740) 65412..66560 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
CHD10_RS23745 (CHD10_23745) 66557..67375 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
CHD10_RS23750 (CHD10_23750) 67372..67830 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
CHD10_RS23755 (CHD10_23755) 68225..68809 - 585 WP_000977522 histidine phosphatase family protein -
CHD10_RS23760 (CHD10_23760) 68869..70071 - 1203 WP_000976348 conjugal transfer protein TraF -
CHD10_RS23765 (CHD10_23765) 70157..70981 - 825 WP_001238942 conjugal transfer protein TraE traE
CHD10_RS23770 (CHD10_23770) 71189..71314 + 126 Protein_79 RNA-guided endonuclease TnpB family protein -
CHD10_RS23775 (CHD10_23775) 71371..72663 - 1293 WP_001350014 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
CHD10_RS23780 (CHD10_23780) 72663..73319 - 657 WP_001193549 A24 family peptidase -
CHD10_RS23785 (CHD10_23785) 73304..73864 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
CHD10_RS23790 (CHD10_23790) 73874..74488 - 615 WP_000908226 type 4 pilus major pilin -
CHD10_RS23795 (CHD10_23795) 74505..75590 - 1086 WP_001208802 type II secretion system F family protein -
CHD10_RS23800 (CHD10_23800) 75603..77156 - 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
CHD10_RS23805 (CHD10_23805) 77167..77619 - 453 WP_001247331 type IV pilus biogenesis protein PilP -
CHD10_RS23810 (CHD10_23810) 77606..78901 - 1296 WP_001545757 type 4b pilus protein PilO2 -
CHD10_RS23815 (CHD10_23815) 78894..80576 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
CHD10_RS23820 (CHD10_23820) 80590..81027 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
CHD10_RS23825 (CHD10_23825) 81027..82094 - 1068 WP_001360287 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   5387 GenBank   NZ_CP030232
Plasmid name   pSA20043041.1 Incompatibility group   IncI1
Plasmid size   87319 bp Coordinate of oriT [Strand]   35551..35639 [-]
Host baterium   Salmonella enterica strain SA20043041

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -