Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104919 |
| Name | oriT_NAS_AN_203|unnamed  |
| Organism | Staphylococcus aureus strain NAS_AN_203 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP062357 (26005..26040 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_NAS_AN_203|unnamed
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3467 | GenBank | WP_001252101 |
| Name | MobA_MobL_IF727_RS13610_NAS_AN_203|unnamed |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 3411 | GenBank | WP_000209435 |
| Name | t4cp2_IF727_RS13545_NAS_AN_203|unnamed |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62671.66 Da Isoelectric Point: 7.6569
>WP_000209435.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2793..14013
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IF727_RS13485 (IF727_13420) | 1..960 | + | 960 | WP_000043161 | replication initiator protein A | - |
| IF727_RS13490 (IF727_13425) | 1065..1253 | - | 189 | WP_001063224 | hypothetical protein | - |
| IF727_RS13495 (IF727_13430) | 1425..2399 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| IF727_RS13500 (IF727_13435) | 2416..2733 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| IF727_RS13690 (IF727_13445) | 3187..3869 | + | 683 | Protein_4 | TrsD/TraD family conjugative transfer protein | - |
| IF727_RS13515 (IF727_13450) | 3890..5902 | + | 2013 | WP_000874919 | DUF87 domain-containing protein | virb4 |
| IF727_RS13520 (IF727_13455) | 5914..7194 | + | 1281 | WP_240022464 | hypothetical protein | trsF |
| IF727_RS13525 (IF727_13460) | 7212..8288 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| IF727_RS13530 (IF727_13465) | 8298..8783 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| IF727_RS13535 (IF727_13470) | 8799..10901 | + | 2103 | WP_240022465 | DNA topoisomerase III | - |
| IF727_RS13540 (IF727_13475) | 10917..11381 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| IF727_RS13545 (IF727_13480) | 11378..13018 | + | 1641 | WP_000209435 | VirD4-like conjugal transfer protein, CD1115 family | - |
| IF727_RS13550 (IF727_13485) | 13096..14013 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| IF727_RS13555 (IF727_13490) | 14030..14422 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| IF727_RS13560 (IF727_13495) | 14479..15210 | + | 732 | WP_240022466 | hypothetical protein | - |
| IF727_RS13565 (IF727_13500) | 15247..15870 | + | 624 | WP_000831307 | hypothetical protein | - |
| IF727_RS13570 (IF727_13505) | 15886..16500 | + | 615 | WP_000527296 | hypothetical protein | - |
| IF727_RS13575 (IF727_13510) | 16569..17840 | + | 1272 | WP_000386737 | hypothetical protein | - |
| IF727_RS13580 (IF727_13515) | 17882..18298 | - | 417 | WP_031895653 | YolD-like family protein | - |
Host bacterium
| ID | 5357 | GenBank | NZ_CP062357 |
| Plasmid name | NAS_AN_203|unnamed | Incompatibility group | - |
| Plasmid size | 29018 bp | Coordinate of oriT [Strand] | 26005..26040 [+] |
| Host baterium | Staphylococcus aureus strain NAS_AN_203 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |