Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104916
• Name: oriT_pSaBSN9S
• Organism: Staphylococcus aureus strain NAS_AN_036
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP062391 (15701..15845 [+], 145 nt)
• oriT length: 145 nt
• IRs (inverted repeats): 105..110, 114..119 (TTTAAA..TTTAAA) 33..39, 44..50 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 145 nt

>oriT_pSaBSN9S
TGTCACAAAAATAAGACAAATTCTACATGTTGTGTCACAAAACTGTGACAAACACAATATATAGTGTCACAAAACTGTGACACTATAGTTTATTGTGGGGTCACTTTAAATTCTTTAAAAACCTTGAAATGGCTGGCTTTGCCAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_IF699_RS14065_pSaBSN9S

• ID: 3466
• GenBank: WP_031864843
• Name: mobP2_IF699_RS14065_pSaBSN9S
• UniProt ID: _
• Length: 382 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 382 a.a.
• Molecular weight: 45738.95 Da
• Isoelectric Point: 9.9348

>WP_031864843.1 MobP2 family relaxase [Staphylococcus aureus]
MPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAIN
KIKSDFSHAQKNKGMMWKDVISFDNSALESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVWS
GAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDES
EPYKDKAMKKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKRQTNL
YKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSNATITKLKIGKCLNQAL
YRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-357)

Protein structure

No available structure.

T4CP

t4cp2_IF699_RS14015_pSaBSN9S

• ID: 3410
• GenBank: WP_223224017
• Name: t4cp2_IF699_RS14015_pSaBSN9S
• UniProt ID: _
• Length: 858 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 858 a.a.
• Molecular weight: 98808.19 Da
• Isoelectric Point: 6.3969

>WP_223224017.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MKQLNTKYTDYIFNFRLDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKA
IPQREHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFAT
TFFSKREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRG
YQVEIFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMI
LALCEETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATV
QAAPDKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENG
IEYEEVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDN
SDITIRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNM
PPIPNLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQ
ITVHSRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMK
YRYQYLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEQENKNINDINQYK
QSNEEGNVAVRVKRLETLKEKVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVSADNEVKEEE
KTKRIEMIQKFIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (549-666)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (143-392)

Protein structure

No available structure.

Host bacterium

• ID: 5354
• GenBank: NZ_CP062391
• Plasmid name: pSaBSN9S
• Incompatibility group: -
• Plasmid size: 37087 bp
• Coordinate of oriT [Strand]: 15701..15845 [+]
• Host baterium: Staphylococcus aureus strain NAS_AN_036

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA14