Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104894
Name   oriT_pCFSAN076215 in_silico
Organism   Salmonella enterica subsp. enterica serovar Anatum strain CFSAN076215
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP033339 (98522..98602 [-], 81 nt)
oriT length   81 nt
IRs (inverted repeats)      42..48, 51..57  (ACCGCGC..GCGCGGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 81 nt

>oriT_pCFSAN076215
GGGGTGTCGGGGCGAAGCCCTGACCAGGTGGTAAATGTATGACCGCGCGTGCGCGGTTATACATTTACACATCCTGTCCCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3464 GenBank   WP_058344438
Name   nikB_EAE34_RS23990_pCFSAN076215 insolico UniProt ID   A0A2T8X2C5
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104560.06 Da        Isoelectric Point: 7.1822

>WP_058344438.1 IncI1-type relaxase NikB [Salmonella enterica]
MNAIIPKKRRDGKSSFEDLVSYVSVRDDLEDEELQALSSVQSELSHRSRFSRLVDYATRLRDESFVSLVD
VMRDGCEWVNFYGVTCFHNCTSLETAAEEMEYTARQARYAKDGTDPVFHYILSWQAHESPRPEQIYDSVR
HTLQALGLSEHQYVSAVHTDTDNLHVHVAVNRVHPDTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
IHAPGSRIVRKTAAERDRQNAWTRGKKQTFREYVSQTAVAGLRSEPVHDWLSLHRRLAEDGLYLTLQNGS
FMVMDGWDRNREGVQLDSFGPSWNGDKLRKKMGEYTPVPKDIFSQVGAPGRYKPEVIAADTRPEKMAETV
NLMQYACRHLGERLPEMAREGKLESCLDIHRTLAEAGLWMRIQHGHLVICDGYDHNQTPVRADSVWPLLT
PENVNQLDGGWQPVPDDIFRQVTPAERFSGRRLESCPASDREWHRMRTGPGPQGAIRRELFSDKESLWGY
SISHCSPQIEEMITRGEFTWQHCHELFARQGLMLQRQHHGLIVVDAFNHEQTPVKASSIHPDLTLDRAEP
QAGPFVNAPADLFDRVKPESRYNPELAVSDQSREDCRRDPVLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERSREIHQVCRLRKSHIRVQYDDPVLRKLHYHIAEVQRMQALIRLKEEVRNERQKLIAEGKWYP
PSYRQWVEAQAVQGDRAAVSQLRGWDYRDRRKDKSRTTTIDRCVILCEPGGTPVYENRGELEARLQKNGS
VRFRDRRTDQFVCTDYGDRVVFHNHHDRNELADKLDLIAPVLFERDPRMGFEPEGNDRQFNQVFAEMVAW
HNVTERTGHGDYTISRPDVDHHREGSERYYRDYINAHTGREASLPSLEHEKRHEPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(63-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A2T8X2C5


Auxiliary protein

ID   1537 GenBank   WP_001281049
Name   WP_001281049_pCFSAN076215 insolico UniProt ID   A0A5T6J7U7
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12698.63 Da        Isoelectric Point: 10.6858

>WP_001281049.1 plasmid mobilization protein MobA [Salmonella enterica]
MSDKRERSGSEKRQKTLLRAVRFSPDEDEIIRKKAEDAGLTVSAYIRSAALNKRVNSRIDDKFLKELMRL
GRMQKHLFVEGKRTGDKEYASVLVAITELSNTVRKQLMEN

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5T6J7U7


T4CP

ID   3408 GenBank   WP_077946969
Name   trbC_EAE34_RS23995_pCFSAN076215 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86963.11 Da        Isoelectric Point: 7.6568

>WP_077946969.1 F-type conjugative transfer protein TrbC [Salmonella enterica]
MSEHRVNPDLIHRTAWGNPLWNALQNLNIYGMCLAGSLVVSFIWPLALPACLLFTLITVLIFSLQRWRCP
LRMPMTLRCADPSQDRMIKRSLFEFWPTLFQYETIKEFPANGIFYVGYQRIRDIGRELWLNIDDLTRHVM
FFAATGGGKTETIFSWIINPLCWGRGFTLVDGKAQNDTARTIWYLSRRFGREDDVEVINFMNGGKSRSEI
ILSGDKSRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGRTMSL
QMLREHMSLEGMAKLYCRGIDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFVTQQSMILARDLGYRLE
GSDSQALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTSATSTATLM
QNTGTKFAGRVVSDDKTARTLKNAAGEEARARMNSLQRQDGVFGEAWIDSPQISILMESKVNVQELIKLH
PGEFFSIFQGESVPSASFYIPDEEKSCSSDPVVINRYISVDAPRLEQLRRLVPRTVQRRIPSPENVSAII
GVLTAKPSHKRRKNRTEPHIIVDTFQQRLAGRQAAMALLEEYDTDINAREMALWETALNTLKTTTREQRR
IRFITLNRPKTPAADEEHQVSVRAERAEINLLSLPKQNYRPAGPSANGSHHKNNSRHEWKGLY

  Protein domains



No domain identified.


  Protein structure



No available structure.




Host bacterium

ID   5332 GenBank   NZ_CP033339
Plasmid name   pCFSAN076215 Incompatibility group   IncI1
Plasmid size   104123 bp Coordinate of oriT [Strand]   98522..98602 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Anatum strain CFSAN076215

Cargo genes

Drug resistance gene   -
Virulence gene   mgtB, mgtC
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -