Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104853 |
| Name | oriT_pSR01  |
| Organism | Staphylococcus aureus strain SR153 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP048644 (21467..21502 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pSR01
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3438 | GenBank | WP_239557439 |
| Name | MobA_MobL_GAU34_RS14955_pSR01 |
UniProt ID | _ |
| Length | 324 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 324 a.a. Molecular weight: 38281.80 Da Isoelectric Point: 7.5058
>WP_239557439.1 MobA/MobL family protein [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKSWNL
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKSWNL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3380 | GenBank | WP_030061486 |
| Name | t4cp2_GAU34_RS14405_pSR01 |
UniProt ID | _ |
| Length | 548 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 548 a.a. Molecular weight: 62899.75 Da Isoelectric Point: 5.9714
>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26897..38498
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GAU34_RS14325 (GAU34_14325) | 21967..22200 | + | 234 | WP_000602212 | hypothetical protein | - |
| GAU34_RS14330 (GAU34_14330) | 22463..23503 | + | 1041 | WP_000073390 | hypothetical protein | - |
| GAU34_RS14335 (GAU34_14335) | 23506..23835 | + | 330 | WP_030061459 | plasmid segregation protein ParR | - |
| GAU34_RS14340 (GAU34_14340) | 23839..24099 | - | 261 | WP_001008214 | helix-turn-helix transcriptional regulator | - |
| GAU34_RS14345 (GAU34_14345) | 24482..25441 | + | 960 | WP_030061463 | replication initiator protein A | - |
| GAU34_RS14350 (GAU34_14350) | 25545..25733 | - | 189 | WP_030061465 | hypothetical protein | - |
| GAU34_RS14355 (GAU34_14355) | 25906..26880 | + | 975 | WP_030061467 | conjugative transfer protein TrsA | - |
| GAU34_RS14360 (GAU34_14360) | 26897..27214 | + | 318 | WP_030061469 | CagC family type IV secretion system protein | virB2 |
| GAU34_RS14960 (GAU34_14370) | 27667..28349 | + | 683 | Protein_31 | TrsD/TraD family conjugative transfer protein | - |
| GAU34_RS14375 (GAU34_14375) | 28370..30382 | + | 2013 | WP_030061475 | DUF87 domain-containing protein | virb4 |
| GAU34_RS14380 (GAU34_14380) | 30394..31257 | + | 864 | WP_239557441 | type VII secretion protein EssB/YukC | trsF |
| GAU34_RS14965 | 31392..31673 | + | 282 | WP_239557442 | hypothetical protein | trsF |
| GAU34_RS14385 (GAU34_14385) | 31691..32767 | + | 1077 | WP_030061480 | CHAP domain-containing protein | trsG |
| GAU34_RS14390 (GAU34_14390) | 32777..33262 | + | 486 | WP_000735570 | TrsH/TraH family protein | - |
| GAU34_RS14395 (GAU34_14395) | 33278..35380 | + | 2103 | WP_030061483 | DNA topoisomerase III | - |
| GAU34_RS14400 (GAU34_14400) | 35396..35860 | + | 465 | WP_000715271 | hypothetical protein | trsJ |
| GAU34_RS14405 (GAU34_14405) | 35857..37503 | + | 1647 | WP_030061486 | VirD4-like conjugal transfer protein, CD1115 family | - |
| GAU34_RS14410 (GAU34_14410) | 37581..38498 | + | 918 | WP_030061488 | conjugal transfer protein TrbL family protein | trsL |
| GAU34_RS14415 (GAU34_14415) | 38515..38910 | + | 396 | WP_000608969 | single-stranded DNA-binding protein | - |
Host bacterium
| ID | 5291 | GenBank | NZ_CP048644 |
| Plasmid name | pSR01 | Incompatibility group | - |
| Plasmid size | 39500 bp | Coordinate of oriT [Strand] | 21467..21502 [+] |
| Host baterium | Staphylococcus aureus strain SR153 |
Cargo genes
| Drug resistance gene | cfr, aac(6')-aph(2'') |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |