Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   104853
Name   oriT_pSR01 in_silico
Organism   Staphylococcus aureus strain SR153
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP048644 (21467..21502 [+], 36 nt)
oriT length   36 nt
IRs (inverted repeats)     _
Location of nic site      29..30
Conserved sequence flanking the
  nic site  
 
 GTGCGCCCTT
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pSR01
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   3438 GenBank   WP_239557439
Name   MobA_MobL_GAU34_RS14955_pSR01 insolico UniProt ID   _
Length   324 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 324 a.a.        Molecular weight: 38281.80 Da        Isoelectric Point: 7.5058

>WP_239557439.1 MobA/MobL family protein [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKSWNL

  Protein domains


Predicted by InterproScan.

(17-216)


  Protein structure



No available structure.




T4CP


ID   3380 GenBank   WP_030061486
Name   t4cp2_GAU34_RS14405_pSR01 insolico UniProt ID   _
Length   548 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 548 a.a.        Molecular weight: 62899.75 Da        Isoelectric Point: 5.9714

>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK

  Protein domains


Predicted by InterproScan.

(3-462)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 26897..38498

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GAU34_RS14325 (GAU34_14325) 21967..22200 + 234 WP_000602212 hypothetical protein -
GAU34_RS14330 (GAU34_14330) 22463..23503 + 1041 WP_000073390 hypothetical protein -
GAU34_RS14335 (GAU34_14335) 23506..23835 + 330 WP_030061459 plasmid segregation protein ParR -
GAU34_RS14340 (GAU34_14340) 23839..24099 - 261 WP_001008214 helix-turn-helix transcriptional regulator -
GAU34_RS14345 (GAU34_14345) 24482..25441 + 960 WP_030061463 replication initiator protein A -
GAU34_RS14350 (GAU34_14350) 25545..25733 - 189 WP_030061465 hypothetical protein -
GAU34_RS14355 (GAU34_14355) 25906..26880 + 975 WP_030061467 conjugative transfer protein TrsA -
GAU34_RS14360 (GAU34_14360) 26897..27214 + 318 WP_030061469 CagC family type IV secretion system protein virB2
GAU34_RS14960 (GAU34_14370) 27667..28349 + 683 Protein_31 TrsD/TraD family conjugative transfer protein -
GAU34_RS14375 (GAU34_14375) 28370..30382 + 2013 WP_030061475 DUF87 domain-containing protein virb4
GAU34_RS14380 (GAU34_14380) 30394..31257 + 864 WP_239557441 type VII secretion protein EssB/YukC trsF
GAU34_RS14965 31392..31673 + 282 WP_239557442 hypothetical protein trsF
GAU34_RS14385 (GAU34_14385) 31691..32767 + 1077 WP_030061480 CHAP domain-containing protein trsG
GAU34_RS14390 (GAU34_14390) 32777..33262 + 486 WP_000735570 TrsH/TraH family protein -
GAU34_RS14395 (GAU34_14395) 33278..35380 + 2103 WP_030061483 DNA topoisomerase III -
GAU34_RS14400 (GAU34_14400) 35396..35860 + 465 WP_000715271 hypothetical protein trsJ
GAU34_RS14405 (GAU34_14405) 35857..37503 + 1647 WP_030061486 VirD4-like conjugal transfer protein, CD1115 family -
GAU34_RS14410 (GAU34_14410) 37581..38498 + 918 WP_030061488 conjugal transfer protein TrbL family protein trsL
GAU34_RS14415 (GAU34_14415) 38515..38910 + 396 WP_000608969 single-stranded DNA-binding protein -


Host bacterium


ID   5291 GenBank   NZ_CP048644
Plasmid name   pSR01 Incompatibility group   -
Plasmid size   39500 bp Coordinate of oriT [Strand]   21467..21502 [+]
Host baterium   Staphylococcus aureus strain SR153

Cargo genes


Drug resistance gene   cfr, aac(6')-aph(2'')
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21